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8.A.84 The Insulin Secretion Regulator TMEM59 (TMEM59) Family 

Glucose-stimulated insulin secretion (GSIS) in pancreatic beta cells is regulated by several proteins including Tmem59l (Transmembrane protein 59 like), Scgn (Secretagogin), Gucy2c (Guanylate cyclase 2c), Slc29a4 (Solute carrier family 29, member 4), Cdhr1 (Cadherin-related family member 1) and Celsr2 (Cadherin EGF LAG seven-pass G-type receptor 2). These genes were knocked down in MIN6c4 cells, and the effects on insulin expression and secretion were analyzed. Suppression of Tmem59l, Scgn, and Gucy2c expression resulted in significantly decreased glucose- and/or KCl-stimulated insulin secretion, while the suppression of Slc29a4 expression resulted in increased insulin secretion. Tmem59l overexpression rescued the phenotype of the Tmem59l knockdown cells, and immunostaining analysis indicated that the TMEM59L protein colocalized with insulin and GM130, a Golgi complex marker. Thus, Tmem59l, Scgn, Gucy2c, and Slc29a4 may regulate GSIS.

References associated with 8.A.84 family:

Chen, Y., R. Feng, G. Luo, J. Guo, Y. Wang, Y. Sun, L. Zheng, and T. Wen. (2018). DCF1 subcellular localization and its function in mitochondria. Biochimie 144: 50-55. 29074393
Gerlach, J.P., I. Jordens, D.V.F. Tauriello, I. van ''t Land-Kuper, J.M. Bugter, I. Noordstra, J. van der Kooij, T.Y. Low, F.X. Pimentel-Muiños, D. Xanthakis, N. Fenderico, C. Rabouille, A.J.R. Heck, D.A. Egan, and M.M. Maurice. (2018). TMEM59 potentiates Wnt signaling by promoting signalosome formation. Proc. Natl. Acad. Sci. USA 115: E3996-E4005. 29632210
Kobayashi, M., E. Yamato, K. Tanabe, F. Tashiro, S. Miyazaki, and J. Miyazaki. (2016). Functional Analysis of Novel Candidate Regulators of Insulin Secretion in the MIN6 Mouse Pancreatic β Cell Line. PLoS One 11: e0151927. 26986842
Ullrich, S., A. Münch, S. Neumann, E. Kremmer, J. Tatzelt, and S.F. Lichtenthaler. (2010). The novel membrane protein TMEM59 modulates complex glycosylation, cell surface expression, and secretion of the amyloid precursor protein. J. Biol. Chem. 285: 20664-20674. 20427278