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9.A.16.1.2
Lysosomal-associated membrane protein 2 precursor, LAMP2a. LAMP2 colocalizes with TM7SF1 (GRP137B) (TC#9.B.123.1.1) (Gao et al., 2012).

Accession Number:P13473
Protein Name:LMP2 aka lamp2a aka LAMP2
Length:410
Molecular Weight:44961.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass type I membrane protein2
Substrate Unfolded proteins

Cross database links:

HOG000230942HOG000230942
Genevestigator: P13473 P13473 P13473
eggNOG: prNOG09849 NOG301998 NOG301998
RefSeq: NP_002285.1    NP_054701.1   
Entrez Gene ID: 3920   
Pfam: PF01299   
OMIM: 300257  phenotype
309060  gene
KEGG: hsa:3920    hsa:3920   

Gene Ontology

GO:0010008 C:endosome membrane
GO:0016021 C:integral to membrane
GO:0005770 C:late endosome
GO:0005765 C:lysosomal membrane
GO:0005624 C:membrane fraction
GO:0005886 C:plasma membrane
GO:0031088 C:platelet dense granule membrane
GO:0031902 C:late endosome membrane
GO:0030168 P:platelet activation
GO:0002576 P:platelet degranulation

References (57)

[1] “Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences.”  Fukuda M.et.al.   3198605
[2] “The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes.”  Sawada R.et.al.   8517882
[3] “Molecular and cellular characterization of Mex-/methylation-resistant phenotype. Gene and cDNA cloning, serum dependence, and tumor suppression of transfectant strains.”  Fritz G.et.al.   8407947
[4] “Complete cDNA sequence of human lysosome-associated membrane protein-2.”  Konecki D.S.et.al.   7999007
[5] “An alternatively spliced form of the human lysosome-associated membrane protein-2 gene is expressed in a tissue-specific manner.”  Konecki D.S.et.al.   7488019
[6] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[7] “The DNA sequence of the human X chromosome.”  Ross M.T.et.al.   15772651
[8] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[9] “Purification and characterization of human lysosomal membrane glycoproteins.”  Mane S.M.et.al.   2912382
[10] “Signal peptide prediction based on analysis of experimentally verified cleavage sites.”  Zhang Z.et.al.   15340161
[11] “The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. Localization on the peptide backbones.”  Carlsson S.R.et.al.   2243102
[12] “Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2.”  Carlsson S.R.et.al.   8323299
[13] “Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.”  Zhang H.et.al.   12754519
[14] “Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.”  Liu T.et.al.   16335952
[15] “Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.”  Chen R.et.al.   19159218
[16] “Glycogen storage diseases presenting as hypertrophic cardiomyopathy.”  Arad M.et.al.   15673802
[17] “Asymptomatic hyperCKemia in a case of Danon disease due to a missense mutation in Lamp-2 gene.”  Musumeci O.et.al.   15907287
[18] “Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences.”  Fukuda M.et.al.   3198605
[19] “The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes.”  Sawada R.et.al.   8517882
[20] “Molecular and cellular characterization of Mex-/methylation-resistant phenotype. Gene and cDNA cloning, serum dependence, and tumor suppression of transfectant strains.”  Fritz G.et.al.   8407947
[21] “Complete cDNA sequence of human lysosome-associated membrane protein-2.”  Konecki D.S.et.al.   7999007
[22] “An alternatively spliced form of the human lysosome-associated membrane protein-2 gene is expressed in a tissue-specific manner.”  Konecki D.S.et.al.   7488019
[23] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[24] “The DNA sequence of the human X chromosome.”  Ross M.T.et.al.   15772651
[25] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[26] “Purification and characterization of human lysosomal membrane glycoproteins.”  Mane S.M.et.al.   2912382
[27] “Signal peptide prediction based on analysis of experimentally verified cleavage sites.”  Zhang Z.et.al.   15340161
[28] “The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. Localization on the peptide backbones.”  Carlsson S.R.et.al.   2243102
[29] “Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2.”  Carlsson S.R.et.al.   8323299
[30] “Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.”  Zhang H.et.al.   12754519
[31] “Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.”  Liu T.et.al.   16335952
[32] “Unifying nomenclature for the isoforms of the lysosomal membrane protein LAMP-2.”  Eskelinen E.L.et.al.   16190986
[33] “Integral and associated lysosomal membrane proteins.”  Schroeder B.et.al.   17897319
[34] “Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.”  Chen R.et.al.   19159218
[35] “Initial characterization of the human central proteome.”  Burkard T.R.et.al.   21269460
[36] “Glycogen storage diseases presenting as hypertrophic cardiomyopathy.”  Arad M.et.al.   15673802
[37] “Asymptomatic hyperCKemia in a case of Danon disease due to a missense mutation in Lamp-2 gene.”  Musumeci O.et.al.   15907287
[38] “Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences.”  Fukuda M.et.al.   3198605
[39] “The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes.”  Sawada R.et.al.   8517882
[40] “Molecular and cellular characterization of Mex-/methylation-resistant phenotype. Gene and cDNA cloning, serum dependence, and tumor suppression of transfectant strains.”  Fritz G.et.al.   8407947
[41] “Complete cDNA sequence of human lysosome-associated membrane protein-2.”  Konecki D.S.et.al.   7999007
[42] “An alternatively spliced form of the human lysosome-associated membrane protein-2 gene is expressed in a tissue-specific manner.”  Konecki D.S.et.al.   7488019
[43] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[44] “The DNA sequence of the human X chromosome.”  Ross M.T.et.al.   15772651
[45] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[46] “Purification and characterization of human lysosomal membrane glycoproteins.”  Mane S.M.et.al.   2912382
[47] “Signal peptide prediction based on analysis of experimentally verified cleavage sites.”  Zhang Z.et.al.   15340161
[48] “The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. Localization on the peptide backbones.”  Carlsson S.R.et.al.   2243102
[49] “Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins lamp-1 and lamp-2.”  Carlsson S.R.et.al.   8323299
[50] “Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.”  Zhang H.et.al.   12754519
[51] “Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.”  Liu T.et.al.   16335952
[52] “Unifying nomenclature for the isoforms of the lysosomal membrane protein LAMP-2.”  Eskelinen E.L.et.al.   16190986
[53] “Integral and associated lysosomal membrane proteins.”  Schroeder B.et.al.   17897319
[54] “Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.”  Chen R.et.al.   19159218
[55] “Initial characterization of the human central proteome.”  Burkard T.R.et.al.   21269460
[56] “Glycogen storage diseases presenting as hypertrophic cardiomyopathy.”  Arad M.et.al.   15673802
[57] “Asymptomatic hyperCKemia in a case of Danon disease due to a missense mutation in Lamp-2 gene.”  Musumeci O.et.al.   15907287
Structure:
2MOF   2MOM     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MVCFRLFPVP GSGLVLVCLV LGAVRSYALE LNLTDSENAT CLYAKWQMNF TVRYETTNKT 
61:	YKTVTISDHG TVTYNGSICG DDQNGPKIAV QFGPGFSWIA NFTKAASTYS IDSVSFSYNT 
121:	GDNTTFPDAE DKGILTVDEL LAIRIPLNDL FRCNSLSTLE KNDVVQHYWD VLVQAFVQNG 
181:	TVSTNEFLCD KDKTSTVAPT IHTTVPSPTT TPTPKEKPEA GTYSVNNGND TCLLATMGLQ 
241:	LNITQDKVAS VININPNTTH STGSCRSHTA LLRLNSSTIK YLDFVFAVKN ENRFYLKEVN 
301:	ISMYLVNGSV FSIANNNLSY WDAPLGSSYM CNKEQTVSVS GAFQINTFDL RVQPFNVTQG 
361:	KYSTAQDCSA DDDNFLVPIA VGAALAGVLI LVLLAYFIGL KHHHAGYEQF