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9.A.6.1.1
The ATP release protein, Mcd4

Accession Number:P36051
Protein Name:Mcd4
Length:919
Molecular Weight:105693.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Endoplasmic reticulum membrane1 / Multi-pass membrane protein2
Substrate ATP

Cross database links:

Genevestigator: P36051
eggNOG: fuNOG05707
HEGENOM: HBG609268
DIP: DIP-8272N
RefSeq: NP_012756.1   
Entrez Gene ID: 853690   
Pfam: PF01663    PF04987   
KEGG: sce:YKL165C   

Gene Ontology

GO:0005789 C:endoplasmic reticulum membrane
GO:0009277 C:fungal-type cell wall
GO:0000324 C:fungal-type vacuole
GO:0000139 C:Golgi membrane
GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0051377 F:mannose-ethanolamine phosphotransferase act...
GO:0005515 F:protein binding
GO:0015867 P:ATP transport
GO:0007047 P:cellular cell wall organization
GO:0006506 P:GPI anchor biosynthetic process

References (11)

[1] “DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae.”  Vandenbol M.et.al.   8091859
[2] “Complete DNA sequence of yeast chromosome XI.”  Dujon B.et.al.   8196765
[3] “MCD4 encodes a conserved endoplasmic reticulum membrane protein essential for glycosylphosphatidylinositol anchor synthesis in yeast.”  Gaynor E.C.et.al.   10069808
[4] “A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast.”  Packeiser A.N.et.al.   10514566
[5] “A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis.”  Storey M.K.et.al.   11470244
[6] “Correct GPI-anchor synthesis is required for the incorporation of endoglucanase/glucanosyltransferase Bgl2p into the Saccharomyces cerevisiae cell wall.”  Kalebina T.S.et.al.   12023081
[7] “ATP uptake in the Golgi and extracellular release require Mcd4 protein and the vacuolar H+-ATPase.”  Zhong X.et.al.   12807869
[8] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[9] “Deletion of MCD 4 involved in glycosylphosphatidylinositol (GPI) anchor synthesis leads to an increase in beta-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae.”  Maneesri J.et.al.   16233801
[10] “Ethanolaminephosphate side chain added to glycosylphosphatidylinositol (GPI) anchor by mcd4p is required for ceramide remodeling and forward transport of GPI proteins from endoplasmic reticulum to Golgi.”  Zhu Y.et.al.   16704983
[11] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MWNKTRTTLL AVGVLFHLFY LWSIFDIYFI SPLVHGMSPY QSTPTPPAKR LFLIVGDGLR 
61:	ADTTFDKVTH PVSGKTEFLA PFIRSLVMNN ATYGISHTRM PTESRPGHVA MIAGFYEDVS 
121:	AVTKGWKSNP VNFDSFFNQS THTYSFGSPD ILPMFKDGAS DPNKVDTWMY DHTFEDFTQS 
181:	SIELDAFVFR HLDQLFHNST LNSTLDYEIR QDGNVFFLHL LGCDTAGHSY RPYSAEYYDN 
241:	VKYIDDQIPI LIDKVNKFFA DDKTAFIFTA DHGMSAFGSH GDGHPNNTRT PLVAWGAGLN 
301:	KPVHNPFPVS DNYTENWELS SIKRNDVKQA DIASLMSYLI GVNYPKNSVG ELPIAYIDGK 
361:	ESDKLAALYN NARSILEQYL VKQDEVIDSQ FFYKEYFKFV EKSHSHYLEE IETLIQRISE 
421:	GENYLEQEAI TLTEELMQIT LEGLHYLTTY NWRFIRTIVT FGFVGWIFFS FIIFLKSFIL 
481:	ENVIDDQKAS PLSHAVFGSI GILLNWILFY QHSPFNFYMY LLFPLYFWSY IFTNRSVLRS 
541:	GIKEFFKGTS PWKRVLITIS IISVYEGIVY GFFHRWTFTL ITNILAFYPF ICGVRELSVN 
601:	ILWIITSVLL STFTLFDAVK IEDLNQIHLA GLLIILSAFY ALYKIHSRIN SYTRAIFAIQ 
661:	ISLVAAMLAV THRSVISLQL RQGLPRESQV AGWIIFFVSL FVMPILHYRK PNNDYKVRLL 
721:	IIYLTFAPSF IILTISFESL FYFLFTSYMV QWIEIENKIK EMKTQKDENW LQVLRVSVIG 
781:	FFLLQVAFFG TGNVASISSF SLESVCRLLP IFDPFLMGAL LMLKLIIPYG LLSTCLGILN 
841:	LKLNFKDYTI SSLIISMSDI LSLNFFYLLR TEGSWLDIGI TISNYCLAIL SSLFMLILEV 
901:	LGHVLLKNVI IQDKTKKTQ