9.B.214 The ER to Golgi Transport Factor (ER/G-TF) Family
Secretory proteins are transported from the endoplasmic reticulum to the Golgi apparatus via COPII- coated intermediates. Yeast Erv29p is a transmembrane protein cycling between these compartments. It is conserved across eukaryotic species, with one ortholog found in each genome studied, including the surf-4 protein in mammals (Foley et al. 2007). Yeast Erv29p acts as a receptor, loading a specific subset of soluble cargo, including glycosylated alpha factor pheromone precursor and carboxypeptidase Y, into vesicles. As the eukaryotic secretory pathway is highly conserved, mammalian surf-4 may perform a similar role in the transport of unknown substrates. Foley et al. 2007 reported the membrane topology of yeast Erv29p, which they concluded contains four transmembrane domains with both termini exposed to the cytosol although the hydropathy plot suggests 7 or 8 TMSs. Two luminal loops may contain a recognition site for hydrophobic export signals on soluble cargo.