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9.B.31.1.1
The PlsY or YgiH protein of 205 aas and 7 established TMSs.  It plays a role in growth in complex media (Yoshimura et al. 2007). PlsY is glycerol 3-phosphate (G3P) acyltransferase which uses acyl-phosphate as the acyl donor.  The 3-d structure is known (Li et al. 2017).

Accession Number:P60782
Protein Name:UPF0078 membrane protein YgiH aka B3059
Length:205
Molecular Weight:22193.00
Species:Escherichia coli [83333]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Unknown

Cross database links:

Genevestigator: P60782
EchoBASE: EB1625
EcoGene: EG11674
eggNOG: COG0344
HEGENOM: HBG734487
RefSeq: AP_003609.1    NP_417531.1   
Entrez Gene ID: 947561   
Pfam: PF02660   
BioCyc: EcoCyc:EG11674-MONOMER    ECOL168927:B3059-MONOMER   
KEGG: ecj:JW3031    eco:b3059   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0004366 F:glycerol-3-phosphate O-acyltransferase acti...
GO:0008654 P:phospholipid biosynthetic process

References (7)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “Amplification of the bacA gene confers bacitracin resistance to Escherichia coli.”  Cain B.D.et.al.   8389741
[4] “Intrinsic and extrinsic approaches for detecting genes in a bacterial genome.”  Borodovsky M.et.al.   7984428
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[6] “Acyl-phosphates initiate membrane phospholipid synthesis in Gram-positive pathogens.”  Lu Y.-J.et.al.   16949372
[7] “Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli.”  Yoshimura M.et.al.   17645809

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSAIAPGMIL IAYLCGSISS AILVCRLCGL PDPRTSGSGN PGATNVLRIG GKGAAVAVLI 
61:	FDVLKGMLPV WGAYELGVSP FWLGLIAIAA CLGHIWPVFF GFKGGKGVAT AFGAIAPIGW 
121:	DLTGVMAGTW LLTVLLSGYS SLGAIVSALI APFYVWWFKP QFTFPVSMLS CLILLRHHDN 
181:	IQRLWRRQET KIWTKFKRKR EKDPE