TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


9.B.35.2.1
Transthyritin-like protein, PucM.  A probable hydrolase in the uredio pathway; not a transporter (Jung et al. 2006; Doniselli et al. 2015).

Accession Number:O32142
Protein Name:PucM aka BSU32460
Length:114
Molecular Weight:12635.00
Species:Bacillus subtilis [1423]
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Unknown

Cross database links:

HEGENOM: HBG445217
RefSeq: NP_391126.2   
Entrez Gene ID: 937977   
Pfam: PF00576   
KEGG: bsu:BSU32460   

Gene Ontology

GO:0033971 F:hydroxyisourate hydrolase activity
GO:0006144 P:purine base metabolic process
GO:0006810 P:transport

References (5)

[1] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[2] “Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.”  Schultz A.C.et.al.   11344136
[3] “Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.”  Yoshida K.et.al.   12823818
[4] “Transthyretin-related proteins function to facilitate the hydrolysis of 5-hydroxyisourate, the end product of the uricase reaction.”  Lee Y.et.al.   16098976
[5] “Structural and functional analysis of PucM, a hydrolase in the ureide pathway and a member of the transthyretin-related protein family.”  Jung D.-K.et.al.   16782815
Structure:
2H0E   2H0F   2H0J     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MGKLTTHILD LTCGKPAANV KIGLKRLGES IMKEVYTNND GRVDVPLLAG EELMSGEYVM 
61:	EFHAGDYFAS KNMNAADQPF LTIVTVRFQL ADPDAHYHIP LLLSPFGYQV YRGS