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9.B.39.1.4
Putative fatty acid translocase, CD36 glycoprotein (FA translocase; FAT/CD36/SR-B2) (Glatz and Luiken 2017; Zhang et al. 2017).  It is a Leukocyte differentiation antigen and adhesin of 472 aas protein with 2 TMSs, one N-terminal and one C-terminal (Schwenk et al. 2010). Studies have shown that TMS 1 plays a role in formation of a homodimeric structure which may be involved in regulating signal transduction (Wei et al. 2017). Uptake of long chain unsaturated fatty acids, eicosapentaenoic acid and docosahexaenoic acid, is facilitated by CD36/SR-B2 (Glatz and Luiken 2017). Glycosylation, ubiquitination and palmitoylation are involved in regulating CD36 stability, while phosphorylation at extracellular sites affect the rate of fatty acid uptake (Luiken et al. 2016). CD36 may facilitate fatty acid uptake by an indirect mechanism (Jay and Hamilton 2016), but fatty acid uptake studies in breast cancer cells is consistent with its role in transport (Zhao et al. 2017).

Accession Number:P16671
Protein Name:Platelet glycoprotein 4
Length:472
Molecular Weight:53053.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate docosahexaenoic acid (DHA, 22:6 n–3), eicosapentaenoic acid (EPA, 20:5 n–3), fatty acids

Cross database links:

Genevestigator: P16671
eggNOG: NOG257244
Entrez Gene ID: 948   
Pfam: PF01130   
KEGG: hsa:948   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0045335 C:phagocytic vesicle
GO:0031092 C:platelet alpha granule membrane
GO:0008289 F:lipid binding
GO:0030169 F:low-density lipoprotein particle binding
GO:0005041 F:low-density lipoprotein receptor activity
GO:0070053 F:thrombospondin receptor activity
GO:0050431 F:transforming growth factor beta binding
GO:0002479 P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
GO:0007155 P:cell adhesion
GO:0044255 P:cellular lipid metabolic process
GO:0019934 P:cGMP-mediated signaling
GO:0030301 P:cholesterol transport
GO:0019915 P:lipid storage
GO:0042953 P:lipoprotein transport
GO:0034383 P:low-density lipoprotein particle clearance
GO:0007263 P:nitric oxide mediated signal transduction
GO:0015911 P:plasma membrane long-chain fatty acid transport
GO:0030168 P:platelet activation
GO:0002576 P:platelet degranulation
GO:0001954 P:positive regulation of cell-matrix adhesion
GO:0010744 P:positive regulation of macrophage derived foam cell differentiation
GO:0044281 P:small molecule metabolic process

References (20)

[1] “CD36 directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes.”  Oquendo P.et.al.   2473841
[2] “Characterization of two alternatively spliced 5'-untranslated exons of the human CD36 gene in different cell types.”  Taylor K.T.et.al.   7693552
[3] “Cloning of the cDNA encoding human platelet CD36: comparison to PCR amplified fragments of monocyte, endothelial and HEL cells.”  Wyler B.et.al.   7505064
[4] “Structural organization of the gene for human CD36 glycoprotein.”  Armesilla A.L.et.al.   7518447
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “Isolation and characterization of platelet glycoprotein IV (CD36).”  Tandon N.N.et.al.   2468669
[7] “Variability of the CD36 gene in West Africa.”  Gelhaus A.et.al.   11668637
[8] “Gene encoding the collagen type I and thrombospondin receptor CD36 is located on chromosome 7q11.2.”  Fernandez-Ruiz E.et.al.   7503937
[9] “Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes.”  Aboulaich N.et.al.   15242332
[10] “Thrombospondin sequence motif (CSVTCG) is responsible for CD36 binding.”  Asch A.S.et.al.   1371676
[11] “CD36 is palmitoylated on both N- and C-terminal cytoplasmic tails.”  Tao N.et.al.   8798390
[12] “Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach.”  Lewandrowski U.et.al.   16263699
[13] “Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry.”  Picariello G.et.al.   18780401
[14] “Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.”  Chen R.et.al.   19159218
[15] “Molecular basis of CD36 deficiency. Evidence that a 478C-->T substitution (proline90-->serine) in CD36 cDNA accounts for CD36 deficiency.”  Kashiwagi H.et.al.   7533783
[16] “Characterization of single-nucleotide polymorphisms in coding regions of human genes.”  Cargill M.et.al.   10391209
[17] “Malaria susceptibility and CD36 mutation.”  Aitman T.J.et.al.   10890433
[18] “Identification of cryptic splice site, exon skipping, and novel point mutations in type I CD36 deficiency.”  Hanawa H.et.al.   11950861
[19] “CD36 polymorphism is associated with protection from cerebral malaria.”  Omi K.et.al.   12506336
[20] “A common haplotype at the CD36 locus is associated with high free fatty acid levels and increased cardiovascular risk in Caucasians.”  Ma X.et.al.   15282206

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FASTA formatted sequence
1:	MGCDRNCGLI AGAVIGAVLA VFGGILMPVG DLLIQKTIKK QVVLEEGTIA FKNWVKTGTE 
61:	VYRQFWIFDV QNPQEVMMNS SNIQVKQRGP YTYRVRFLAK ENVTQDAEDN TVSFLQPNGA 
121:	IFEPSLSVGT EADNFTVLNL AVAAASHIYQ NQFVQMILNS LINKSKSSMF QVRTLRELLW 
181:	GYRDPFLSLV PYPVTTTVGL FYPYNNTADG VYKVFNGKDN ISKVAIIDTY KGKRNLSYWE 
241:	SHCDMINGTD AASFPPFVEK SQVLQFFSSD ICRSIYAVFE SDVNLKGIPV YRFVLPSKAF 
301:	ASPVENPDNY CFCTEKIISK NCTSYGVLDI SKCKEGRPVY ISLPHFLYAS PDVSEPIDGL 
361:	NPNEEEHRTY LDIEPITGFT LQFAKRLQVN LLVKPSEKIQ VLKNLKRNYI VPILWLNETG 
421:	TIGDEKANMF RSQVTGKINL LGLIEMILLS VGVVMFVAFM ISYCACRSKT IK