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9.B.67.5.1
O-antigen polymerase, RfaL (WaaL).  A 12 TMS topology has been confirmed (Pan et al., 2012).

Accession Number:P27243
Protein Name:RfaL
Length:419
Molecular Weight:46878.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Unknown

Cross database links:

Genevestigator: P27243
EchoBASE: EB1394
EcoGene: EG11424
eggNOG: COG3307
HEGENOM: HBG086555
DIP: DIP-10673N
RefSeq: AP_004169.1    NP_418079.1   
Entrez Gene ID: 948148   
Pfam: PF04932   
BioCyc: EcoCyc:EG11424-MONOMER    ECOL168927:B3622-MONOMER    MetaCyc:EG11424-MONOMER   
KEGG: ecj:JW3597    eco:b3622   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008754 F:O antigen ligase activity
GO:0009244 P:lipopolysaccharide core region biosynthetic...

References (5)

[1] “Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium.”  Klena J.D.et.al.   1624462
[2] “Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.”  Sofia H.J.et.al.   8041620
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLTSFKLHSL KPYTLKSSMI LEIITYILCF FSMIIAFVDN TFSIKIYNIT AIVCLLSLIL 
61:	RGRQENYNIK NLILPLSIFL IGLLDLIWYS AFKVDNSPFR ATYHSYLNTA KIFIFGSFIV 
121:	FLTLTSQLKS KKESVLYTLY SLSFLIAGYA MYINSIHEND RISFGVGTAT GAAYSTMLIG 
181:	IVSGVAILYT KKNHPFLFLL NSCAVLYVLA LTQTRATLLL FPIICVAALI AYYNKSPKKF 
241:	TSSIVLLIAI LASIVIIFNK PIQNRYNEAL NDLNSYTNAN SVTSLGARLA MYEIGLNIFI 
301:	KSPFSFRSAE SRAESMNLLV AEHNRLRGAL EFSNVHLHNE IIEAGSLKGL MGIFSTLFLY 
361:	FSLFYIAYKK RALGLLILTL GIVGIGLSDV IIWARSIPII IISAIVLLLV INNRNNTIN