1.A.38 The Golgi pH Regulator (GPHR) Family

The organelles within secretory and endocytotic pathways in mammalian cells have acidified lumens, and regulation of their acidic pH is critical for the trafficking, processing and glycosylation of cargo proteins and lipids, as well as the morphological integrity of the organelles. Maeda et al. (2008) described a novel protein involved in Golgi acidification. Mutant cells defective in Golgi acidification exhibited delayed protein transport, impaired glycosylation and Golgi disorganization. Using expression cloning, a novel Golgi-resident multi-transmembrane protein, named Golgi pH regulator (GPHR), was identified. After reconstitution in planar lipid bilayers, GPHR exhibited voltage-dependent anion-channel activity that may function in counterion conductance. Thus, GPHR modulates Golgi functions through regulation of acidification. It is reported to be homologous to the G-protein coupled receptor 89B of humans (NP_057418). Its function is reviewed by Edwards and Kahl (2010). It has a central DUF3735 domain, a C-terminal ABA_GREP domain, and 9 putative TMSs.

This family belongs to the .



Edwards JC. and Kahl CR. (2010). Chloride channels of intracellular membranes. FEBS Lett. 584(10):2102-11.

Maeda, Y., T. Ide, M. Koike, Y. Uchiyama, and T. Kinoshita. (2008). GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus. Nat. Cell Biol. 10: 1135-1145.


TC#NameOrganismal TypeExample

The Golgi pH regulator, GPHR


GPHR of Cricetulus griseus (B2ZXD5)


Uncharacterized protein of 367 aas and 8 TMSs.

UP of Entamoeba histolytica


TC#NameOrganismal TypeExample

10 TMS homologue (826 aas)


10 TMS homologue of Leishmania mexicana (E9AL43)


TC#NameOrganismal TypeExample

4-5 TMS homologue (398 aas)


4-5 TMS homologue of Plasmodium yoelii (Q7RQA4)