1.A.72 The Mer Superfamily
The MerF protein encoded on plasmid pMER327/419 is an 81 residue polypeptide with two putative TMSs (Barkay et al., 2003). It catalyzes uptake of Hg2+ in preparation for reduction by mercuric reductase. The MerF gene is found on mercury resistant plasmids from many Gram-negative bacteria, but the sequence of the protein from these plasmids is the same. Its two TMSs show limited sequence similarity with the first two TMSs of MerT (TC#1.A.72.3) and MerC (TC# 1.A.72.4). Some members of the MerF family have been designated MerH (Wilson et al., 2000).
The MerTP permeases catalyze uptake into bacterial cells of Hg2+ in preparation for its reduction by the MerA mercuric reductase. The Hgo produced by MerA is volatile and passively diffuses out of the cell. The merT and merP genes are found on mercury resistance plasmids and transposons of Gram-negative and Gram-positive bacteria but are also chromosomally encoded in some bacteria. MerT consists of about 130 amino acids and has 3 transmembrane helical segments. Evidence for direct interactions between the cytoplasmic face of MerT and theN-terminus of MerA have been presented (Schué et al. 2009). Operon analyses have been reported by Barkay et al., 2003; Miller,1999; Velasco et al., 1999
MerP is a periplasmic Hg2+ -binding receptor of about 70-80 amino acids, synthesized with a cleavable N-terminal leader. It is homologous to the N-terminal heavy metal binding domains of the Copper-and cadmium-transporting P-type ATPases. The 3-D structure of MerP from Ralstonia metallidurans has been solved to 2 Å resolution (Serre et al., 2004; Qian et al.,1998). It is 91 aas long with its leader sequence, is monomeric, and binds a single Hg2+ion. Hg2+ is bound to a sequence GMTCXXC found in metallochaperones as well as metal-transporting ATPases. The fold is βαββαβ, called the ''ferridoxin-like fold''.
MerT homologues have been identified in which the 3 TMS MerT is fused to a MerP ''heavy metal associated'' (HMA) domain possibly via a linker region that includes a fourth TMS (see 1.A.72.3.3). HMA domains of ~30 aas are found in MerP, copper chaperone proteins, mercuric reductase, and at the N-termini of both copper and heavy metal P-type ATPases, sometimes in multiple copies.
The MerC protein encoded on the IncJ plasmid pMERPH of the Shewanella putrefaciens mercuric resistance operon is 137 amino acids in length and possesses four putative transmembrane α-helical spanners (TMSs). It has been shown to bind and take up Hg2+ ions. merC genes are encoded on several plasmids of Gram-negative bacteria and may also be chromosomally encoded. MerC proteins are homologous to other bacterial Hg2+ bacterial transporters ( Inoue et al., 1990; Peters et al., 1991; Mok et al., 2011; Yamaguchi et al., 2007).
The merE gene of transposon Tn21, a pE4 plasmid that contained the merR gene of plasmid pMR26 from Pseudomonas strain K-62, and the merE gene of Tn21 from the Shigella flexneri plasmid NR1 (R100) conferred hypersensitivity to CH3Hg2+ and Hg2+, taking up significantly more CH3Hg2+ and Hg2+ than the isogenic strain (Kiyono et al. 2009). The MerE protein encoded by pE4 was localized in the membrane cell fraction, but not in the soluble fraction. Kiyono et al. (2009) suggested that the merE gene is a broad mercury transporter mediating the transport of both CH3Hg2+ and Hg2+ across the bacterial membrane.
The common origin of all Mer superfamily members has been established (Mok et al., 2011). The common elements are included in TMSs 1-2.
The transport reaction catalyzed by Mer Superfamily members is:
Hg2+ or methyl-Hg2+ (out) → Hg2+ or methyl-Hg2+ (in)
Yamaguchi, A., Tamang D., and Saier M. (2007). Mercury Transport in Bacteria. [DOI: 10.1007/s11270-007-9334-z]
MerF of plasmid pMER327/419 of Pseudomonas aeruginosa
Heavy metal transporter
HM transporter of Arcobacter butzleri (A8EUY8)
MerT (97aas)/MerP (93aas) (in a single operon with a transglutaminase (COG1305)).
MerTP of Haemophilus influenzae
Hg2+ transporter, MerH (171aas; 4 TMSs) (transports mercuric ions via a pair of essential cysteine residues, but only when coexpressed with the mercuric reductase) (Schué et al., 2009).
MerH of Mycobacterium marinum (B2I419)
MerT/P of Ralstonia eutropha (Q6UP69)
Putative MerT-MerP fusion protein of 200 aas (3 TMSs)
MerT-MerP of Chryseobacterium gleum (C0YI47)
Putative MerT-MerP fusion protein of 199 aas (3-4 TMSs)
MerT-MerP of Methylacidiphilum infernorum (B3DYY6)
Putative MerT-MerP fusion protein of 196 aas (3 TMSs)
MerT-MerP of Spirosoma linguale (D2QV66)
Mercuric ion uptake system, MerT-P/MerP
MerT-P/MerP of Tenacibaculum discolor
MerT-P (204aas; H6WCN3)
MerP (113aas; H6WCN4)
MerC of the IncJ plasmid pMERPH of Shewanella putrefaciens
MerC homologue (129aas; 4 TMSs)
MerC homologue of Gemmatimonas aurantiaca
The Mercuric ion (Hg2+) uptake transporter, MerE (78aas; 2 TMSs).
MerE of transposon Tn21 of E. coli (Q57069)