1.B.28 The Plastid Outer Envelope Porin of 24 kDa (OEP24) Family

OEP24 is a high conductance, homooligomeric porin in the outer envelopes of pea chloroplasts, etioplasts and non-green root plastids. It forms a voltage-dependent, high conductance, slightly cationic-selective ion channel in reconstituted proteoliposomes. OEP24 transports triosephosphates, dicarboxylates, positively and negatively charged amino acids, sugars, ATP, and inorganic phosphate and thus exhibits low solute specificity. Structural predictions suggest the presence of seven amphipathic β-strands in a β-barrel arrangement. It contains 213 aas and shows a single homologue in Arabidopsis thaliana (212 aas; 60% identity). OEP24 can functionally replace mitochondrial VDAC (MPP family; TC #1.B.28) when the OEP24 gene is expressed in Saccharomyces cerevisiae. Thus, in yeast, it is targeted to the outer mitochondrial membrane, inserts properly, and functionally replaces VDAC. Nevertheless, it shows no sequence similarity to VDAC.

The generalized reaction catalyzed by OEP24 is:

solute (cytoplasm)solute (chloroplast intermembrane space)



This family belongs to the Outer Membrane Pore-forming Protein (OMPP) Superfamily III.

 

References:

Bölter, B. and J. Soll. (2001). Ion channels in the outer membranes of chloroplasts and mitochondria: open doors or regulated gates? EMBO J. 20: 1-6.

Pohlmeyer, K., J. Soll, R. Grimm, K. Hill, and R. Wagner. (1998). A high-conductance solute channel in the chloroplastic outer envelope from Pea. Plant Cell 10: 1207-1216.

Röhl T., M. Mitzkus and J. Soll. (1999). The outer envelope protein OEP24 from pea chloroplasts can functionally replace the mitochondrial VDAC in yeast. FEBS Lett. 460: 591-594.

Ulrich, T., L.E. Gross, M.S. Sommer, E. Schleiff, and D. Rapaport. (2012). Chloroplast β-barrel proteins are assembled into the mitochondrial outer membrane in a process that depends on the TOM and TOB complexes. J. Biol. Chem. 287: 27467-27479.

Examples:

TC#NameOrganismal TypeExample
1.B.28.1.1

High conductance chloroplast outer envelope OEP24 porin (Pohlmeyer et al. 1998).

Plants

OEP24 of Pisum sativum

 
1.B.28.1.2

OEP24 homologue

Plants

OEP24 homologue of Selaginella moellendorffii

 
1.B.28.1.3

OEP24 homologue

Green algae

OEP24 homologue of Chlorella variabilis

 
1.B.28.1.4

Oep24 of 213 aas.  High-conductance voltage-dependent solute channel with a slight selectivity for cations transporting triosephosphates, dicarboxylic acids, ATP, inorganic phosphate (Pi), sugars, and positively or negatively charged amino acids. The central mitochondrial components that mediate the import of yeast β-barrel proteins can deal with precursors of chloroplast β-barrel proteins (Ulrich et al. 2012).

Oep24 of Arabidopsis thaliana (Mouse-ear cress)

 
Examples:

TC#NameOrganismal TypeExample
1.B.28.2.1

Putative OEP24 homologue of 286 aas

Plants

OEP24 homologue of Coccomyxa subellipsoidea