1.C.47 The Insect/Fungal Defensin (Insect/Fungal Defensin) Family
Many insect (arthropod) defensins have been sequenced and shown to form ion channels in artificial membranes. Their precursor proteins are secreted and digested to the active peptides that can be bacterocidal and toxic to specific eukaryotic cells (Kourie and Shorthouse, 2000).
The defensins with a conserved cysteine-stabilized alpha-helix and beta-sheet (CSαβ) structural motif are a group of unique antimicrobial polypeptides widely distributed in plants and animals. Recently, one defensin-like peptide (DLP) with high degrees of sequence and structural similarity to defensins from ancient arthropods and molluscs has been identified in a saprophytic fungus (Mygind et al., 2005). This poses an important question regarding the evolutionary relationships of this class of effectors of innate immunity in three eukaryotic kingdoms.
Zhu (2008) reported the computational identification of six families of fungal DLPs in which three known defensin types (antibacterial ancient invertebrate-type defensins (AITDs), antibacterial classical insect-type defensins (CITDs), and antifungal plant/insect-type defensins (PITDs)) were clearly assigned. Sharing of these defensin types between animals and fungi supported their closer evolutionary relationship, consistent with the Opisthokonta Hypothesis. Conservation of the PITDs across three eukaryotic kingdoms suggests an earlier origin (Zhu, 2008).
The generalized transport reaction catalyzed by defensins is:
ions and small molecules (in) ions and small molecules (out)
Soft tick Defensin A (73aas; 1 TMS)
Defensin A of Ornithodoros moubata (Q9BLJ3)
Defensin-A (37-aas) (Charlet et al., 1996;Zhu, 2008).
Defensin-A of Mytilis edulis (P81610)
Gigasin-2 (95 aas) (Zhu, 2008).
Gigasin-2 of Crassostrea gigas (Q6H9L9)
Atesin-3 (71aas) (Zhu, 2008).
Atesin-3 of Aspergillus terreus (B1NJ41).
Scapularisin preproprotein of 101 aas and 1 TMS, IscW.
IscW of Ixodes scapularis (Black-legged tick) (Deer tick)
L-Plectasin (40aas, 1 TMS); precursor (90aas, 2 TMSs). 3-d structure known (3E7R_L; 1ZFUA) (Mygind et al., 2005; Zhu, 2008) (43% identical to 1.C.47.1.1).
L-Plectasin precursor of Pseudoplectania nigrella (Q53I06)
Panscorpine (Scorpine; scorpin) of 94 aas
Scorpine of Pandinus imperator
Potassium channel toxin BmTXK-beta; BmKLK; BmTX K-beta; BmTXKbeta of 90 aas
BmTXK of Mesobuthus martensii
Beta-KTx-like peptide of 79 aas
Beta-KTx-like peptide of Pandinus cavimanus (Tanzanian red clawed scorpion)
Male-specific defensin of 79 aas
Defensin of Haemaphysalis longicornis