1.E.29  The Holin Hol44 (Hol44) Family

Oenococcus oeni cells are intrinsically resistant to the secreted endolysin from oenophage fOg44 (Lys44). Experiments with several antimicrobials support the hypothesis that the full activity of Lys44 requires sudden ion-nonspecific dissipation of the proton motive force, an event undertaken by the fOg44 holin in the phage infection context (Nascimento et al. 2008).



This family belongs to the Holin Superfamily V.

 

References:

Goh, S., P.F. Ong, K.P. Song, T.V. Riley, and B.J. Chang. (2007). The complete genome sequence of Clostridium difficile phage phiC2 and comparisons to phiCD119 and inducible prophages of CD630. Microbiology 153: 676-685.

Nascimento, J.G., M.C. Guerreiro-Pereira, S.F. Costa, C. São-José, and M.A. Santos. (2008). Nisin-triggered activity of Lys44, the secreted endolysin from Oenococcus oeni phage fOg44. J. Bacteriol. 190: 457-461.

Oki, M., M. Kakikawa, S. Nakamura, E.T. Yamamura, K. Watanabe, M. Sasamoto, A. Taketo, and K. Kodaira. (1997). Functional and structural features of the holin HOL protein of the Lactobacillus plantarum phage phi gle: analysis in Escherichia coli system. Gene 197: 137-145.

Examples:

TC#NameOrganismal TypeExample
1.E.29.1.1

p36 holin of Clostridial phage Phi C2.  Functional in E. coli (Goh et al. 2007).

Firmicute

p36 holin of phage Phi C2

 
1.E.29.1.2

Holin of Clostridial phage Phi CD119 (Goh et al. 2007).

Firmicute phage

Holin of clostridial phage Phi CD119

 
1.E.29.1.3

Putative holin

Firmicutes

Putative holin of Lactobacillus plantarum

 
1.E.29.1.4

Putative holin (87 aas)

Firmicutes

Putative holin of Tetagenococcus halophilus

 
Examples:

TC#NameOrganismal TypeExample
1.E.29.2.1

Holin, Hol44 of Oenococcus phage fOg44 (118 aas; 3 TMSs)

Firmicute viruses

Hol44 of phage fOg44

 
1.E.29.2.2

Putative holin

Firmicutes

Putative holin of Leuconostoc kimchii

 
1.E.29.2.3

Holin (97aas)

Firmicute viruses

Holin of Leuconostoc gasicomitatum

 
1.E.29.2.4

Lactobacillus phage phi g1e holin (Oki et al. 1997).

Firmicute viruses

Lactobacillus phage phi g1e holin

 
1.E.29.2.5

Putative holin of 78 aas and 3 TMSs

Firmicutes

Putative holin of Pelotomaculum thermopropionicum

 
1.E.29.2.6

Putative holin of 81 aas and 3 TMSs.

Firmicutes

Holin of Sporosarcina newyorkensis

 
Examples:

TC#NameOrganismal TypeExample
1.E.29.3.1

Putative holin of 81 aas and 3 TMSs

Firmicutes

Putative holin of Brevibacillus laterosporus