1.E.31 The SPP1 Holin (SPP1 Holin) Family

The SPP1 Holin Family consists of proteins of 90 - 160 aas with two TMSs



This family belongs to the .

 

References:

Aunpad, R. and W. Panbangred. (2012). Evidence for two putative holin-like peptides encoding genes of Bacillus pumilus strain WAPB4. Curr. Microbiol. 64: 343-348.

Bon, J., N. Mani, and R.K. Jayaswal. (1997). Molecular analysis of lytic genes of bacteriophage 80 alpha of Staphylococcus aureus. Can. J. Microbiol. 43: 612-616.

Durmaz, E. and T.R. Klaenhammer. (2007). Abortive phage resistance mechanism AbiZ speeds the lysis clock to cause premature lysis of phage-infected Lactococcus lactis. J. Bacteriol. 189: 1417-1425.

Fernandes, S. and C. São-José. (2017). Probing the function of the two holin-like proteins of bacteriophage SPP1. Virology 500: 184-189.

Krogh, S., S.T. Jørgensen, and K.M. Devine. (1998). Lysis genes of the Bacillus subtilis defective prophage PBSX. J. Bacteriol. 180: 2110-2117.

Kyogoku, K. and J. Sekiguchi. (1996). Cloning and sequencing of a new holin-encoding gene of Bacillus licheniformis. Gene 168: 61-65.

Examples:

TC#NameOrganismal TypeExample
1.E.31.1.1

Lactococcus lactis phage Phi31 holin of the Holin SPP1 or Phage_holin family (Durmaz and Klaenhammer 2007).

Firmicute phage

Holin of phage Phi31

 
1.E.31.1.10

Putative holin

Firmicute phage

Putative holin of Brochithrix phage A9

 
1.E.31.1.11

XhlB holin of 109 aas; belongs to the SPP1 family.

Firmicutes

XhlB of Staphylococcus epidermidis

 
1.E.31.1.12

XhlB holin of 87 aas and 2 TMSs (Aunpad and Panbangred 2012).

Firmicutes

XhlB holin of Bacillus pumilus

 
1.E.31.1.13

Two component holin, requiring two non-homologous small proteins, the gene 26 product (O48472; 82 aas, 2 TMSs, a member of family TC# 1.E.31) and the gene 24.1 product (O48470; 83 aas; 1 C-terminal TMS, not homologous to members of family 1.E.31) (Fernandes and São-José 2017).

Holin of Bacillus phage SPP1
Orf26
Orf24.1

 
1.E.31.1.14

Two component holin, requiring two non-homologous small proteins, one of 87 aas and 2 TMSs (E0U1U5), a member of family TC# 1.E.31, and another gene product, XhlA, of 89 aas with 1 C-terminal TMS (P39798), not homologous to members of family 1.E.31 (Fernandes and São-José 2017).

Holin of Bacillus phage PBSX
Orf of 87 aas and 2 TMSs (E0U1U5)
Orf of 89 aas and 1 C-terminal TMS (P39798)

 
1.E.31.1.2

Prophage L54a holin of the SPP1 family

Firmicute prophage

Holin of Staphylococcus aureus

 
1.E.31.1.3

Holin of 74 aas and 2 TMSs

Firmicutes

Holin of Bacillus pumilus

 
1.E.31.1.4

Holin

Firmicutes

Holin of Streptococcus gallolyticus

 
1.E.31.1.5

Holin

Actinobacteria

Holin of Bifidobacterium calenulatum

 
1.E.31.1.6

Holin XhlB (Krogh et al. 1998).

Firmicutes

Holin XhlB of Bacillus subtilis prophage PBSX

 
1.E.31.1.7

Holin XpaG2 (Kyogoku and Sekiguchi 1996).

Firmicutes

X[aG2 pf Bacillus licheniformis

 
1.E.31.1.8

Holin-like protein

Actinobacteria

Holin-like protein of Bifidobacterium longum

 
1.E.31.1.9

Putative holin

Firmicute phage

Putative holin of Bacillus phage BPS13

 
Examples:

TC#NameOrganismal TypeExample
1.E.31.2.1

Putative holin of 77 aas and 2 TMSs

Bacilli (Firmicutes)

Putative holin of Bacillus thuringiensis

 
1.E.31.2.2

Putative holin of 77 aas and 2 TMS

Bacilli (Firmicutes)

Putative holin of Bacillus cereus

 
1.E.31.2.3

Holin

Firmicutes

Holin of Bacillus cereus

 
1.E.31.2.4

Putative holin

Actinobacteria

Putative holin of Brevibacterium laterosporus

 
Examples:

TC#NameOrganismal TypeExample
1.E.31.3.1

Putative holin (Orf041) of Staphylococcal phage 2683

Firmicute phage

Holin of Staphylococcus aureus phage 2683

 
1.E.31.3.2

Putative firmicute holin

Firmicutes

Putative holin of Solibacillus silvestris