2.A.15 The Betaine/Carnitine/Choline Transporter (BCCT) Family

Proteins of the BCCT family are found in Gram-negative and Gram-positive bacteria and archaea. Their common functional feature is that they all transport molecules with a quaternary ammonium group [R-N (CH3)3]. The BCCT family proteins vary in length between 481 and 706 amino acyl residues and possess 12 putative transmembrane α-helical spanners (TMSs).  The x-ray structures (see next paragraph) reveal two 5 TMS repeats with the total number of TMSs being 10. These porters catalyze bidirectional uniport or are energized by pmf-driven or smf-driven proton or sodium ion symport, respectively, or else by substrate:substrate antiport. Some of these permeases exhibit osmosensory and osmoregulatory properties inherent to their polypeptide chains.  The BCCT family has been reviewed (Ziegler et al. 2010).

Schulze et al. (2010) reported the structures of the sodium-independent carnitine/butyrobetaine antiporter CaiT from Proteus mirabilis (PmCaiT) at 2.3 Å and from E. coli (EcCaiT) at 3.5 Å resolution. Most members of the BCCT family are Na+- or H+-dependent, whereas EcCaiT is Na+- and H+-independent. The three-dimensional architecture of CaiT resembles that of the Na+-dependent transporters LeuT and BetP, but in CaiT, a methionine sulphur takes the place of the Na+ to coordinate the substrate in the central transport site, accounting for Na+ independence. Both CaiT structures show the fully open, inward-facing conformation, and thus complete the set of functional states that describe the alternating access mechanism. EcCaiT contains two bound butyrobetaine substrate molecules, one in the central transport site, the other in an extracellular binding pocket. In the structure of PmCaiT, a tryptophan side chain occupies the transport site, and access to the extracellular site is blocked. Binding of both substrates to CaiT reconstituted into proteoliposomes is cooperative, with Hill coefficients of up to 1.7, indicating that the extracellular site is regulatory. Schulze et al. (2010) proposed a mechanism whereby the occupied regulatory site increases the binding affinity of the transport site and initiates substrate translocation.

Most secondary-active transporters transport their substrates using an electrochemical ion gradient, but the carnitine transporter (CaiT) is an ion-independent, l-carnitine/gamma-butyrobetaine antiporter. Crystal structures of CaiT from E. coli and Proteus mirabilis revealed the inverted five-transmembrane-helix repeat similar to that in the amino acid/Na+ symporter, LeuT. Kalayil et al.(2013) showed that mutations of arginine 262 (R262) made CaiT Na+-dependent with increased transport activity in the presence of a membrane potential, in agreement with substrate/Na+ cotransport. R262 also plays a role in substrate binding by stabilizing the partly unwound TM1' helix.

Modeling CaiT from P. mirabilis in the outward-open and closed states on the corresponding structures of the related symporter BetP revealed alternating orientations of the buried R262 side chain, which mimic sodium binding and unbinding in the Na+-coupled substrate symporters. A similar mechanism may be operative in other Na+/H+-independent transporters, in which a positively

charged amino acid replaces the cotransported cation. The oscillation of the R262 side chain in CaiT indicates how a positive charge triggers the change between outward-open and inward-open conformations (Kalayil et al., 2013). 

The generalized transport reactions catalyzed by members of the BCCT family are:

Substrate (out) + nH+ (out) → Substrate (in) + nH+ (in)

Substrate (out) + Na+ (out) → Substrate (in) + Na+ (in)

Substrate1 (out) + Substrate2 (in) → Substrate1 (in) + Substrate2 (out)

Substrate (out) ⇌ Substrate (in)

Substrate = a quaternary amine



This family belongs to the APC Superfamily.

 

References:

Boscari, A., K. Mandon, L. Dupont, M.-C. Poggi, and D. Le Rudulier. (2002). BetS is a major glycine betaine/proline betaine transporter required for early osmotic adjustment in Sinorhizobium meliloti. J. Bacteriol. 184: 2654-2663.

Chen, C. and G.A. Beattie. (2008). Pseudomonas syringae BetT is a low-affinity choline transporter that is responsible for superior osmoprotection by choline over glycine betaine. J. Bacteriol. 190(8): 2717-2725.

Eichler, K., F. Bourgis, A. Buchet, H.P. Kleber, and M.A. Mandrand-Berthelot. (1994). Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli. Mol. Microbiol. 13: 775-786.

Gärtner, R.M., C. Perez, C. Koshy, and C. Ziegler. (2011). Role of Bundle Helices in a Regulatory Crosstalk in the Trimeric Betaine Transporter BetP. J. Mol. Biol. 414: 327-336.

Ge, L., C. Perez, I. Waclawska, C. Ziegler, and D.J. Muller. (2011). Locating an extracellular K+-dependent interaction site that modulates betaine-binding of the Na+-coupled betaine symporter BetP. Proc. Natl. Acad. Sci. USA 108: E890-898.

Güler, G., R.M. Gärtner, C. Ziegler, and W. Mäntele. (2016). Lipid-Protein Interactions in the Regulated Betaine Symporter BetP Probed by Infrared Spectroscopy. J. Biol. Chem. 291: 4295-4307.

Hohle, T.H. and M.R. O'Brian. (2009). The mntH gene encodes the major Mn2+ transporter in Bradyrhizobium japonicum and is regulated by manganese via the Fur protein. Mol. Microbiol. 72: 399-409.

Jung, H., M. Buchholz, J. Clausen, M. Nietschke, A. Revermann, R. Schmid, and K. Jung. (2002). CaiT of Escherichia coli, a new transporter catalyzing L-carnitine/γ-butyrobetaine exchange. J. Biol. Chem. 277: 39251-39258.

Kalayil, S., S. Schulze, and W. Kühlbrandt. (2013). Arginine oscillation explains Na+ independence in the substrate/product antiporter CaiT. Proc. Natl. Acad. Sci. USA 110: 17296-17301.

Kappes, R., B. Kempf, and E. Bremer. (1996). Three transport systems for the osmoprotectant glycine betaine operate in Bacillus subtilis: Characterization of OpuD. J. Bacteriol. 178: 5071-5079.

Kempf, B. and E. Bremer. (1998). Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch. Microbiol. 170: 319-330.

Khafizov K., Perez C., Koshy C., Quick M., Fendler K., Ziegler C. and Forrest LR. (2012). Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP. Proc Natl Acad Sci U S A. 109(44):E3035-44.

Lamark, T., I. Kaasen, M.W. Eshoo, P. Falkenberg, J. McDougall, and A.R. Strom. (1991). DNA sequence and analysis of the betgenes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli. Mol. Microbiol. 5: 1049-1064.

Perez, C., B. Faust, A.R. Mehdipour, K.A. Francesconi, L.R. Forrest, and C. Ziegler. (2014). Substrate-bound outward-open state of the betaine transporter BetP provides insights into Na+ coupling. Nat Commun 5: 4231.

Peter, H., B. Weil, A. Burkovski, R. Krämer, and S. Morbach. (1998). Corynebacterium glutamicumis equipped with four secondary carriers for compatible solutes: identification, sequencing, and characterization of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine betaine carrier, EctP. J. Bacteriol. 180: 6005-6012.

Ressl, S., A.C. Terwisscha van Scheltinga, C. Vonrhein, V. Ott, and C. Ziegler. (2009). Molecular basis of transport and regulation in the Na+/betaine symporter BetP. Nature 458: 47-52.

Rübenhagen, R., H. Rönsch, H. Jung, R. Krämer, and S. Morbach. (2000). Osmosensor and osmoregulator properties of the betaine carrier BetP from Corynebacterium glutamicumin proteoliposomes. J. Biol. Chem. 275: 735-741.

Saier, M.H., Jr., B.H. Eng, S. Fard, J. Garg, D.A. Haggerty, W.J. Hutchinson, D.L. Jack, E.C. Lai, H.J. Liu, D.P. Nusinew, A.M. Omar, S.S. Pao, I.T. Paulsen, J.A. Quan, M. Sliwinski, T.-T. Tseng, S. Wachi, and G.B. Young. (1999). Phylogenetic characterization of novel transport protein families revealed by genome analyses. Biochim. Biophys. Acta 1422: 1-56.

Schulze, S., S. Köster, U. Geldmacher, A.C. Terwisscha van Scheltinga, and W. Kühlbrandt. (2010). Structural basis of Na+-independent and cooperative substrate/product antiport in CaiT. Nature 467: 233-236.

Tang, L., L. Bai, W.H. Wang, and T. Jiang. (2010). Crystal structure of the carnitine transporter and insights into the antiport mechanism. Nat Struct Mol Biol 17: 492-496.

Tsai, C.J., K. Khafizov, J. Hakulinen, L.R. Forrest, L.R. Forrest, R. Krämer, W. Kühlbrandt, and C. Ziegler. (2011). Structural asymmetry in a trimeric Na+/betaine symporter, BetP, from Corynebacterium glutamicum. J. Mol. Biol. 407: 368-381.

Tøndervik, A. and A.R. Strøm. (2007). Membrane topology and mutational analysis of the osmotically activated BetT choline transporter of Escherichia coli. Microbiology 153: 803-813.

Ziegler, C., E. Bremer, and R. Krämer. (2010). The BCCT family of carriers: from physiology to crystal structure. Mol. Microbiol. 78: 13-34.

Examples:

TC#NameOrganismal TypeExample
2.A.15.1.1

Glycine betaine:Na+ symporter (also transports dimethylsulfonioacetate and dimethylsulfoniopropionate)

Bacteria

OpuD of Bacillus subtilis

 
2.A.15.1.10

Glycine betaine transporter, BetP. The x-ray structure is known (3PO3; 2WIT; Ressl et al., 2009). Regulatory crosstalk in the trimeric BetP has been reported (Gärtner et al., 2011). An extracellular K+ -dependent interaction site modulates betaine-binding (Ge et al., 2011). The porter is trimeric and exhibits structural asymmetry (Tsai et al., 2011). The C-terminal domain is involved in osmosensing and is trimeric like wild-type BetP.  The two Na+ binding sites are between TMSs 1 and 8 in the first and second 5 TMS repeats, and between the equivalent TMSs 6 and 3 in the second and first repeats, respectively (Khafizov et al. 2012). interdependent binding of betaine and two sodium ions is observed during the coupling process. All three sites undergo progressive reshaping and dehydration during the alternating-access cycle, with the most optimal coordination of all substrates found in the closed state (Perez et al. 2014). BetP is active and regulated only when negatively charged lipids such as phosphatidyl glycerol are present, and the mechanism has been discussed (Güler et al. 2016).  The K+-sensing C-terminal domain results in K+-dependent cooperative betaine-binding (Ge et al. 2011).

Bacteria 

BetP of Corynebacterium glutamicum (P54582)

 
2.A.15.1.11Glycine betaine transporter BetL (Glycine betaine-Na(+) symporter)BacteriaBetL of Listeria monocytogenes
 
2.A.15.1.2

Ectosine/glycine betaine/proline:Na+ symporter

Bacteria

EctP of Corynebacterium glutamicum

 
2.A.15.1.3

Low affinity (0.9 mM), high efficiency, choline/glycine betaine:H+ symporter, BetT (Chen and Beattie, 2007)

Bacteria

BetT of Pseudomonas syringae (Q4ZLW8)

 
2.A.15.1.4

The high-affinity, proton- or sodium-driven, secondary symporter, BetT.  The cytoplasmic C-terminal domain of plays a role in the regulation of BetT activity; C-terminal truncations cause BetT to be permanently locked in a low-transport-activity mode. (Tøndervik and Strøm 2007).

Bacteria

BetT of E. coli (P0ABC9)

 
2.A.15.1.5

Glycine-betaine/proline-betaine:Na+ symporter, BetS

Bacteria

BetS of Sinorhizobium meliloti (Q92WM0)

 
2.A.15.1.6

The glycine betaine, dimethylsulfoniopropionate:Na+ symporter (Ziegler et al., 2010).

Bacteria

Dddt of Psyohrobacter sp. J466 (D0U567)

 
2.A.15.1.7

The ectoine/glycine:Na+ symporter, LcoP (Ziegler et al., 2010).

Bacteria

LcoP of Corynebacterium glutamicum (Q8NN75)

 
2.A.15.1.8

The ectoine/hydroxyectoine:Na+ symporter, EctT (Ziegler et al., 2010).

Bacteria

EctT of Virgibacillus pantothenticus (Q93AK1)

 
2.A.15.1.9

High affinity glycine betaine uptake system

Bacteria

Glycine betaine transporter of Acinetobacter baylyi (Q6F754)

 
Examples:

TC#NameOrganismal TypeExample
2.A.15.2.1

Carnitine:γ-butyrobetaine antiporter.  The x-ray structure is known at 3.5 Å resolution (Schulze et al., 2010).  The structure reveals a homotrimer where each protomer has 12 TMSs with 4 L-carnitine molecules outlining the pathway.  There is a central binding site and another in the intracellular vestibule (Tang et al. 2010).

Bacteria

CaiT of E. coli (P31553)

 
2.A.15.2.2

The L-carnitine:γ-butyrobetaine antiporter, CaiT.  The x-ray structure is known at 2.3 Å resolution (Schulze et al., 2010).

 

Bacteria

CaiT of Proteus mirabilis (B4EY22)

 

 
2.A.15.2.3

Uncharacterized transporter, YeaV

Bacteria

YeaV of Escherichia coli O157:H7

 
Examples:

TC#NameOrganismal TypeExample