2.A.21 The Solute:Sodium Symporter (SSS) Family

Members of the SSS family catalyze solute:Na+ symport. The solutes transported may be sugars, amino acids, organo cations such as choline, nucleosides, inositols, vitamins, urea or anions, depending on the system. Members of the SSS family have been identified in bacteria, archaea and animals, and all functionally well-characterized members normally catalyze solute uptake via Na+ symport. The human placental multivitamin symporter cotransports an anionic vitamin with two Na+. In the rabbit Na+:D-glucose cotransporter, SGLT1, the glucose translocation pathway probably involves TMSs 10-13, and the binding site for the inhibitor, phlorizin, involves loop 13 (residues 604-610). Cation binding in the N-terminal domain may induce transport-related conformational changes. A conserved tyrosine in the first transmembrane segment of solute:sodium symporters is involved in Na+-coupled substrate co-transport (Mazier et al., 2011).  Mechanistic aspects of Na+ binding sites in LeuT-like fold symporters has been discussed in detail (Perez and Ziegler 2013).

In the human homologue (hSGLT1), H+ can replace Na+, but the apparent affinity for glucose reduces 20x from 0.3 mM to 6 mM. The apparent affinity for H+ is 6 μM, 1000x higher than for Na+ (6 mM). The transport stoichiometry is 1 glucose:2 Na+ or H+. If Asp204 is replaced by glutamate (D204E), the apparent affinity for H+ increases >20x with no change in apparent Na+ affinity. The D204N or D204C mutation promotes phlorizin-sensitive H+ currents that are 10x greater than Na+ currents, and the glucose:H+ stoichiometry is then as great as 1:145. The mutant system thus behaves as a glucose-gated H+ channel.

Proteins of the SSS vary in size from about 400 residues to about 700 residues and probably possess thirteen to fifteen putative transmembrane helical spanners (TMSs). They generally share a core of 13 TMSs, but different members of the family may have different numbers of TMSs. A 13 TMS topology with a periplasmic N-terminus and a cytoplasmic C-terminus has been experimentally determined for the proline:Na+ symporter, PutP, of E. coli. Residues important for substrate and Na+ binding in PutP are found in TMSs 2, 7 and 9 as well as adjacent loops (Jung, 2002). A 14 TMS topology with periplasmic N- and C-termini has been established for the V. parahaemolyticus SglT carrier. SglT transports sugar:Na with a 1:1 stoichiometry. However, MctP of Rhizobium leguminosarum may take up monocarboxylates via an H+ symport mechanism as a dependency on Na+ could not be demonstrated and uptake was strongly inhibited by 10 μM CCCP.

Faham et al., 2008 reported the crystal structure of a member of the solute sodium symporters (SSS), the Vibrio parahaemolyticus sodium/galactose symporter (vSGLT). The approximately 3.0 angstrom structure contains 14 transmembrane (TM) helices in an inward-facing conformation with a core structure of inverted repeats of 5 TM helices (TM2 to TM6 and TM7 to TM11). Galactose is bound in the center of the core, occluded from the outside solutions by hydrophobic residues. The architecture of the core is similar to that of the leucine transporter (LeuT) (TC#2.A.22.4.2) from the NSS family. Modeling the outward-facing conformation based on the LeuT structure, in conjunction with biophysical data, provided insight into structural rearrangements for active transport (Faham et al., 2008).

Some bacterial sensor kinases (2.A.21.9.1 and 2.A.22.9.2) have N-terminal, 12 TMS, sensor domains that regulate the C-terminal kinase domains. The latter are homologous to the kinase domain of NtrB (Pao and Saier, 1995). The N-terminal sensor domains are homologous, but distantly related to members of the SSS. The closest homologues are PutP of E. coli (2.A.21.2.1) and PanF of E. coli (2.A.21.1.1). Homologous regulatory domains are found in Agrobacterium, Mesorhizobium, Sinorhizobium, Vibrio cholera and Bacillus species. While it is clear that these domains function as sensors, it is not known if they also transport the small molecules they sense.

The generalized transport reaction catalyzed by the members of this family is:

solute (out) + nNa+ (out) → solute (in) + nNa+ (in)



This family belongs to the APC Superfamily.

 

References:

Althoff, T., H. Hentschel, J. Luig, H. Schütz, M. Kasch, and R.K. Kinne. (2006). Na+-D-glucose cotransporter in the kidney of Squalus acanthias: molecular identification and intrarenal distribution. Am. J. Physiol. Regul Integr Comp Physiol 290: R1094-1104.

Althoff, T., H. Hentschel, J. Luig, H. Schütz, M. Kasch, and R.K. Kinne. (2007). Na+ -D-glucose cotransporter in the kidney of Leucoraja erinacea: molecular identification and intrarenal distribution. Am. J. Physiol. Regul Integr Comp Physiol 292: R2391-2399.

Anba-Mondoloni, J., S. Chaillou, M. Zagorec, and M.C. Champomier-Vergès. (2013). Catabolism of N-acetylneuraminic acid, a fitness function of the food-borne lactic acid bacterium Lactobacillus sakei, involves two newly characterized proteins. Appl. Environ. Microbiol. 79: 2012-2018.

Aouameur, R., S. Da Cal, P. Bissonnette, M.J. Coady, and J.Y. Lapointe. (2007). SMIT2 mediates all myo-inositol uptake in apical membranes of rat small intestine. Am. J. Physiol. Gastrointest. Liver. Physiol. 293(6):G1300-G1307.

Barwick, K.E., J. Wright, S. Al-Turki, M.M. McEntagart, A. Nair, B. Chioza, A. Al-Memar, H. Modarres, M.M. Reilly, K.J. Dick, A.M. Ruggiero, R.D. Blakely, M.E. Hurles, and A.H. Crosby. (2012). Defective presynaptic choline transport underlies hereditary motor neuropathy. Am J Hum Genet 91: 1103-1107.

Borghese, R. and D. Zannoni. (2010). Acetate permease (ActP) Is responsible for tellurite (TeO32-) uptake and resistance in cells of the facultative phototroph Rhodobacter capsulatus. Appl. Environ. Microbiol. 76: 942-944.

Borghese, R., L. Canducci, F. Musiani, M. Cappelletti, S. Ciurli, R.J. Turner, and D. Zannoni. (2016). On the role of a specific insert in acetate permeases (ActP) for tellurite uptake in bacteria: Functional and structural studies. J Inorg Biochem 163: 103-109.

Borghese, R., S. Cicerano, and D. Zannoni. (2011). Fructose increases the resistance of Rhodobacter capsulatus to the toxic oxyanion tellurite through repression of acetate permease (ActP). Antonie Van Leeuwenhoek 100: 655-658.

Bracher, S., C.C. Schmidt, S.I. Dittmer, and H. Jung. (2016). Core Transmembrane Domain 6 Plays a Pivotal Role in the Transport Cycle of the Sodium/Proline Symporter PutP. J. Biol. Chem. 291: 26208-26215.

Bracher, S., K. Guérin, Y. Polyhach, G. Jeschke, S. Dittmer, S. Frey, M. Böhm, and H. Jung. (2016). Glu311 in External Loop 4 of the Sodium/Proline Transporter PutP is Crucial for External Gate Closure. J. Biol. Chem. [Epub: Ahead of Print]

Chen ML., Yi L., Jin X., Xie Q., Zhang T., Zhou X., Chang H., Fu YJ., Zhu JD., Zhang QY. and Mi MT. (2013). Absorption of resveratrol by vascular endothelial cells through passive diffusion and an SGLT1-mediated pathway. J Nutr Biochem. 24(11):1823-9.

Coady, M.J., B. Wallendorff, D.G. Gagnon, and J.-Y. Lapointe. (2002). Identification of a novel Na+/myo-inositol cotransporter. J. Biol. Chem. 277: 35219-35224.

Darrouzet, E., S. Lindenthal, D. Marcellin, J.L. Pellequer, and T. Pourcher. (2014). The sodium/iodide symporter: State of the art of its molecular characterization. Biochim. Biophys. Acta. 1838: 244-253.

de Carvalho, F.D. and M. Quick. (2011). Surprising substrate versatility in SLC5A6: Na+-coupled I- transport by the human Na+/multivitamin transporter (hSMVT). J. Biol. Chem. 286: 131-137.

De la Vieja, A., M.D. Reed, C.S. Ginter, and N. Carrasco. (2007). Amino acid residues in transmembrane segment IX of the Na+/I- symporter play a role in its Na+ dependence and are critical for transport activity. J. Biol. Chem. 282: 25290-25298.

Dohán, O., C. Portulano, C. Basquin, A. Reyna-Neyra, L.M. Amzel, and N. Carrasco. (2007). The Na+/I symporter (NIS) mediates electroneutral active transport of the environmental pollutant perchlorate. Proc. Natl. Acad. Sci. U.S.A. 104: 20250-20255.

Dus, M., M. Ai, and G.S. Suh. (2013). Taste-independent nutrient selection is mediated by a brain-specific Na+ /solute co-transporter in Drosophila. Nat Neurosci 16: 526-528.

Elías, A., W. Díaz-Vásquez, M.J. Abarca-Lagunas, T.G. Chasteen, F. Arenas, and C.C. Vásquez. (2015). The ActP acetate transporter acts prior to the PitA phosphate carrier in tellurite uptake by Escherichia coli. Microbiol Res 177: 15-21.

Erokhova, L., A. Horner, N. Ollinger, C. Siligan, and P. Pohl. (2016). The Sodium Glucose Cotransporter SGLT1 Is an Extremely Efficient Facilitator of Passive Water Transport. J. Biol. Chem. 291: 9712-9720.

Eskandari, S., D.D.F. Loo, G. Dai, O. Levy, E.M. Wright, and N. Carrasco. (1997). Thyroid Na+/I- symporter: mechanism, stoichiometry, and specificity. J. Biol. Chem. 272: 27230-27238.

Faham, S., A. Watanabe, G.M. Besserer, D. Cascio, A. Specht, B.A. Hirayama, E.M. Wright, and J. Abramson. (2008). The crystal structure of a sodium galactose transporter reveals mechanistic insights into Na+/sugar symport. Science 321: 810-814.

Fisher, D.J., R.E. Fernández, N.E. Adams, and A.T. Maurelli. (2012). Uptake of biotin by Chlamydia Spp. through the use of a bacterial transporter (BioY) and a host-cell transporter (SMVT). PLoS One 7: e46052.

Frank, H., N. Gröger, M. Diener, C. Becker, T. Braun, and T. Boettger. (2008). Lactaturia and loss of sodium-dependent lactate uptake in the colon of SLC5A8-deficient mice. J. Biol. Chem. 283: 24729-24737.

Gimenez, R., M.F. Nuñez, J. Badia, J. Aguilar, and L. Baldoma. (2003). The gene yjcG, cotranscribed with the gene acs, encodes an acetate permease in Escherichia coli. J. Bacteriol. 185: 6448-6455.

Gopal, E., S. Miyauchi, P.M. Martin, S. Ananth, P. Roon, S.B. Smith, and V. Ganapathy. (2007). Transport of nicotinate and structurally related compounds by human SMCT1 (SLC5A8) and its relevance to drug transport in the mammalian intestinal tract. Pharm Res 24: 575-584.

Haga, T. (2014). Molecular properties of the high-affinity choline transporter CHT1. J Biochem 156: 181-194.

Halestrap, A.P. (2013). Monocarboxylic acid transport. Compr Physiol 3: 1611-1643.

Hopkins AP., Hawkhead JA. and Thomas GH. (2013). Transport and catabolism of the sialic acids N-glycolylneuraminic acid and 3-keto-3-deoxy-D-glycero-D-galactonononic acid by Escherichia coli K-12. FEMS Microbiol Lett. 347(1):14-22.

Hosie, A.H., D. Allaway, and P.S. Poole. (2002). A monocarboxylate permease of Rhizobium leguminosarum is the first member of a new subfamily of transporters. J. Bacteriol. 184: 5436-5448.

Huc-Brandt, S., D. Marcellin, F. Graslin, O. Averseng, L. Bellanger, P. Hivin, E. Quemeneur, C. Basquin, V. Navarro, T. Pourcher, and E. Darrouzet. (2011). Characterisation of the purified human sodium/iodide symporter reveals that the protein is mainly present in a dimeric form and permits the detailed study of a native C-terminal fragment. Biochim. Biophys. Acta. 1808: 65-77.

Jackowski, S. and J.H. Alix. (1990). Cloning, sequence, and expression of the pantothenate permease (panF) gene of Escherichia coli. J. Bacteriol. 172: 3842-3848.

Jiang, X., D.D. Loo, B.A. Hirayama, and E.M. Wright. (2012). The importance of being aromatic: π interactions in sodium symporters. Biochemistry 51: 9480-9487.

Johnson, D.A., S.G. Tetu, K. Phillippy, J. Chen, Q. Ren, and I.T. Paulsen. (2008). High-throughput phenotypic characterization of Pseudomonas aeruginosa membrane transport genes. PLoS Genet 4: e1000211.

Jung, H. (2002). The sodium/substrate symporter family: structural and functional features. FEBS Lett. 529: 73-77.

Jung, H., D. Hilger, and M. Raba. (2012). The Na+/L-proline transporter PutP. Front Biosci 17: 745-759.

Jung, H., R. Rübenhagen, S. Tebbe, K. Leifker, N. Tholema, M. Quick, and R. Schmid. (1998). Topology of the Na+/proline transporter of Escherichia coli. J. Biol. Chem. 273: 26400-26407.

Kashiwagi, K. and K. Igarashi. (2011). Identification and assays of polyamine transport systems in Escherichia coli and Saccharomyces cerevisiae. Methods Mol Biol 720: 295-308.

Kojima, S., A. Bohner, and N. von Wirén. (2006). Molecular mechanisms of urea transport in plants. J. Membr. Biol. 212: 83-91.

Kojima, S., A. Bohner, B. Gassert, L. Yuan, and N. von Wirén. (2007). AtDUR3 represents the major transporter for high-affinity urea transport across the plasma membrane of nitrogen-deficient Arabidopsis roots. Plant J. 52: 30-40.

Korycinski M., Albrecht R., Ursinus A., Hartmann MD., Coles M., Martin J., Dunin-Horkawicz S. and Lupas AN. (2015). STAC--A New Domain Associated with Transmembrane Solute Transport and Two-Component Signal Transduction Systems. J Mol Biol. 427(20):3327-39.

Li, W., J.P. Nicola, L.M. Amzel, and N. Carrasco. (2013). Asn441 plays a key role in folding and function of the Na+/I- symporter (NIS). FASEB J. 27: 3229-3238.

Liu GW., Sun AL., Li DQ., Athman A., Gilliham M. and Liu LH. (2015). Molecular identification and functional analysis of a maize (Zea mays) DUR3 homolog that transports urea with high affinity. Planta. 241(4):861-74.

Liu, T., B. Lo, P. Speight, and M. Silverman. (2008). Transmembrane IV of the high-affinity sodium-glucose cotransporter participates in sugar binding. Am. J. Physiol. Cell Physiol. 295: C64-72.

Mayer, F.L., D. Wilson, I.D. Jacobsen, P. Miramón, K. Große, and B. Hube. (2012). The Novel Candida albicans Transporter Dur31 Is a Multi-Stage Pathogenicity Factor. PLoS Pathog 8: e1002592.

Mazier, S., M. Quick, and L. Shi. (2011). Conserved tyrosine in the first transmembrane segment of solute:sodium symporters is involved in Na+-coupled substrate co-transport. J. Biol. Chem. 286: 29347-29355.

Mérigout, P., M. Lelandais, F. Bitton, J.P. Renou, X. Briand, C. Meyer, and F. Daniel-Vedele. (2008). Physiological and transcriptomic aspects of urea uptake and assimilation in Arabidopsis plants. Plant Physiol. 147: 1225-1238.

Miyauchi S., Gopal E., Babu E., Srinivas SR., Kubo Y., Umapathy NS., Thakkar SV., Ganapathy V. and Prasad PD. (2010). Sodium-coupled electrogenic transport of pyroglutamate (5-oxoproline) via SLC5A8, a monocarboxylate transporter. Biochim Biophys Acta. 1798(6):1164-71.

Miyauchi, S., E. Gopal, Y.-J. Fei, and V. Ganapathy. (2004). Functional identification of SLC5A8, a tumor suppressor down-regulated in colon cancer, as a Na+-coupled transporter for short-chain fatty acids. J. Biol. Chem. 279: 13293-13296.

Moses, S., T. Sinner, A. Zaprasis, N. Stöveken, T. Hoffmann, B.R. Belitsky, A.L. Sonenshein, and E. Bremer. (2012). Proline utilization by Bacillus subtilis: uptake and catabolism. J. Bacteriol. 194: 745-758.

Naftalin, R.J. (2008). Osmotic water transport with glucose in GLUT2 and SGLT. Biophys. J. 94: 3912-3923.

Nagata, K. and Y. Hata. (2006). Substrate specificity of a chimera made from Xenopus SGLT1-like protein and rabbit SGLT1. Biochim. Biophys. Acta. 1758: 747-754.

Nicola, J.P., C. Basquin, C. Portulano, A. Reyna-Neyra, M. Paroder, and N. Carrasco. (2009). The Na+/I- symporter mediates active iodide uptake in the intestine. Am. J. Physiol. Cell Physiol. 296: C654-662.

Nishijyo, T., D. Haas, and Y. Itoh. (2001). The CbrA-CbrB two-component regulatory system controls the utilization of multiple carbon and nitrogen sources in Pseudomonas aeruginosa. Mol. Microbiol. 40: 917-931.

Okuda T., Osawa C., Yamada H., Hayashi K., Nishikawa S., Ushio T., Kubo Y., Satou M., Ogawa H. and Haga T. (2012). Transmembrane topology and oligomeric structure of the high-affinity choline transporter. J Biol Chem. 287(51):42826-34.

Okuda, T. and T. Haga. (2000). Functional characterization of the human high-affinity choline transporter. FEBS Lett. 484: 92-97.

Okuda, T., T. Haga, Y. Kanai, H. Endou, T. Ishihara, and I. Katsura. (2000). Identification and characterization of the high-affinity choline transporter. Nature Neurosci. 3: 120-125.

Pao, G.M. and M.H. Saier, Jr. (1995). Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution. J. Molec. Evol. 40: 136-154.

Paroder-Belenitsky, M., M.J. Maestas, O. Dohán, J.P. Nicola, A. Reyna-Neyra, A. Follenzi, E. Dadachova, S. Eskandari, L.M. Amzel, and N. Carrasco. (2011). Mechanism of anion selectivity and stoichiometry of the Na+/I- symporter (NIS). Proc. Natl. Acad. Sci. USA 108: 17933-17938.

Perez, C. and C. Ziegler. (2013). Mechanistic aspects of sodium-binding sites in LeuT-like fold symporters. Biol Chem 394: 641-648.

Plata C., C.R. Sussman, A. Sindic, J.O. Liang, D.B. Mount, Z.M. Josephs, M.H. Chang, M.F. Romero. (2007). Zebrafish Slc5a12 encodes an electroneutral sodium monocarboxylate transporter (SMCTn). A comparison with the electrogenic SMCT (SMCTe/Slc5a8). J. Biol. Chem. 282: 11996-12009.

Portulano, C., M. Paroder-Belenitsky, and N. Carrasco. (2014). The Na+/I(-) Symporter (NIS): Mechanism and Medical Impact. Endocr Rev 35: 106-149.

Prasad, P.D., H. Wang, R. Kekuda, T. Fujita, Y.-J. Fei, L.D. Devoe, F.H. Leibach, and V. Ganapathy. (1998). Cloning and functional expression of a cDNA encoding a mammalian sodium-dependent vitamin transporter mediating the uptake of pantothenate, biotin, and lipoate. J. Biol. Chem. 273: 7501-7506.

Quick, M., D.D.F. Loo, and E.M. Wright. (2001). Neutralization of a conserved amino acid residue in the human Na+/glucose transporter (hSGLT1) generates a glucose-gated H+ channel. J. Biol. Chem. 276: 1728-1734.

Raba, M., S. Dunkel, D. Hilger, K. Lipiszko, Y. Polyhach, G. Jeschke, S. Bracher, J.P. Klare, M. Quick, H. Jung, and H.J. Steinhoff. (2014). Extracellular Loop 4 of the Proline Transporter PutP Controls the Periplasmic Entrance to Ligand Binding Sites. Structure 22: 769-780.

Reizer, J., A. Reizer, and M.H. Saier, Jr. (1991). The Na+/pantothenate symporter (PanF) of Escherichia coli is homologous to the Na+/proline symporter (PutP) of E. coli and the Na+/glucose symporters of mammals. Res. Microbiol. 141: 1069-1072.

Reizer, J., A. Reizer, and M.H. Saier, Jr. (1994). A functional superfamily of sodium/solute symporters. Biochim. Biophys. Acta 1197: 133-166.

Rivera-Ordaz, A., S. Bracher, S. Sarrach, Z. Li, L. Shi, M. Quick, D. Hilger, R. Haas, and H. Jung. (2013). The Sodium/Proline Transporter PutP of Helicobacter pylori. PLoS One 8: e83576.

Rodionov, D.A., C. Yang, X. Li, I.A. Rodionova, Y. Wang, A.Y. Obraztsova, O.P. Zagnitko, R. Overbeek, M.F. Romine, S. Reed, J.K. Fredrickson, K.H. Nealson, and A.L. Osterman. (2010). Genomic encyclopedia of sugar utilization pathways in the Shewanella genus. BMC Genomics 11: 494.

Sanguinetti, M., S. Amillis, S. Pantano, C. Scazzocchio, and A. Ramón. (2014). Modelling and mutational analysis of Aspergillus nidulans UreA, a member of the subfamily of urea/H⁺ transporters in fungi and plants. Open Biol 4: 140070.

Sarker, R.I., W. Ogawa, T. Shimamoto, T. Shimamoto, and T. Tsuchiya. (1997). Primary structure and properties of the Na+/glucose symporter (SglS) of Vibrio parahaemolyticus. J. Bacteriol. 179: 1805-1808.

Sasseville, L.J., J.P. Longpré, B. Wallendorff, and J.Y. Lapointe. (2014). The transport mechanism of the human sodium/myo-inositol transporter 2 (SMIT2/SGLT6), a member of the LeuT structural family. Am. J. Physiol. Cell Physiol. 307: C431-441.

Sasseville, L.J., M. Morin, M.J. Coady, R. Blunck, and J.Y. Lapointe. (2016). The Human Sodium-Glucose Cotransporter (hSGLT1) Is a Disulfide-Bridged Homodimer with a Re-Entrant C-Terminal Loop. PLoS One 11: e0154589.

Severi, E., A.H. Hosie, J.A. Hawkhead, and G.H. Thomas. (2010). Characterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters. FEMS Microbiol. Lett. 304: 47-54.

Spiegelhalter, F. and E. Bremer. (1998). Osmoregulation of the opuE proline transport gene from Bacillus subtilis: contributions of the sigma A- and sigma B-dependent stress-responsive promoters. Mol. Microbiol. 29: 285-296.

Su, X., R. Li, K.F. Kong, and J.S. Tsang. (2016). Transport of haloacids across biological membranes. Biochim. Biophys. Acta. 1858: 3061-3070.

Tatsumi KI., Fujiwara H., Tanaka S. and Amino N. (201). Characterization of Thr-354 in the human sodium/iodide symporter (NIS) by site-directed mutagenesis. Endocr J. 57(11):997-9.

Turk, E. and E.M. Wright. (1997). Membrane topology motifs in the SGLT cotransporter family. J. Membr. Biol. 159: 1-20.

Turk, E., O. Kim, J. le Coutre, J.P. Whitelegge, S. Eskandari, J.T. Lam, M. Kreman, G. Zampighi, K.F. Faull, and E.M. Wright. (2000). Molecular characterization of Vibrio parahaemolyticus vSGLT: a model for sodium-coupled sugar cotransporters. J. Biol. Chem. 275: 25711-25716.

Turk, E., O.K. Gasymov, S. Lanza, J. Horwitz, and E.M. Wright. (2006). A reinvestigation of the secondary structure of functionally active vSGLT, the vibrio sodium/galactose cotransporter. Biochemistry 45: 1470-1479.

Uemura, T., K. Kashiwagi, and K. Igarashi. (2007). Polyamine uptake by DUR3 and SAM3 in Saccharomyces cerevisiae. J. Biol. Chem. 282: 7733-7741.

Vadlapudi AD., Vadlapatla RK., Pal D. and Mitra AK. (2012). Functional and molecular aspects of biotin uptake via SMVT in human corneal epithelial (HCEC) and retinal pigment epithelial (D407) cells. AAPS J. 14(4):832-42.

Vadlapudi, A.D., R.K. Vadlapatla, and A.K. Mitra. (2012). Sodium dependent multivitamin transporter (SMVT): a potential target for drug delivery. Curr Drug Targets 13: 994-1003.

Vallari, D.S. and C.O. Rock. (1985). Isolation and characterization of Escherichia coli pantothenate permease (panF) mutants. J. Bacteriol. 164: 136-142.

von Blohn, C., B. Kempf, R.M. Kappes, and E. Bremer. (1997). Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B. Mol. Microbiol. 25: 175-187.

Wang X., Xu X., Ma M., Zhou W., Wang Y. and Yang L. (2012). pH-dependent channel gating in connexin26 hemichannels involves conformational changes in N-terminus. Biochim Biophys Acta. 1818(5):1148-1157.

Wang, H., W. Huang, Y.-J. Fei, H. Xia, T.L. Yang-Feng, F.H. Leibach, L.D. Devoe, V. Ganapathy, and P.D. Prasad. (1999). Human placental Na+-dependent multivitamin transporter. J. Biol. Chem. 274: 14875-14883.

Wang, X.X., J. Levi, Y. Luo, K. Myakala, M. Herman-Edelstein, L. Qiu, D. Wang, Y. Peng, A. Grenz, S. Lucia, E. Dobrinskikh, V.D. D''Agati, H. Koepsell, J.B. Kopp, A. Rosenberg, and M. Levi. (2017). SGLT2 Expression is increased in Human Diabetic Nephropathy: SGLT2 Inhibition Decreases Renal Lipid Accumulation, Inflammation and the Development of Nephropathy in Diabetic Mice. J. Biol. Chem. [Epub: Ahead of Print]

Wargacki, A.J., E. Leonard, M.N. Win, D.D. Regitsky, C.N. Santos, P.B. Kim, S.R. Cooper, R.M. Raisner, A. Herman, A.B. Sivitz, A. Lakshmanaswamy, Y. Kashiyama, D. Baker, and Y. Yoshikuni. (2012). An engineered microbial platform for direct biofuel production from brown macroalgae. Science 335: 308-313.

Watanabe, A., S. Choe, V. Chaptal, J.M. Rosenberg, E.M. Wright, M. Grabe, and J. Abramson. (2010). The mechanism of sodium and substrate release from the binding pocket of vSGLT. Nature 468: 988-991.

Wilson MC., Meredith D., Bunnun C., Sessions RB. and Halestrap AP. (2009). Studies on the DIDS-binding site of monocarboxylate transporter 1 suggest a homology model of the open conformation and a plausible translocation cycle. J Biol Chem. 284(30):20011-21.

Xie, Z., E. Turk, and E.M. Wright. (2000). Characterization of the Vibrio parahaemolyticus Na+/glucose cotransporter: a bacterial member of the sodium/glucose transporter (SGLT) family. J. Biol Chem. 275: 25959-25964.

Yoshida, K., H. Yamaguchi, M. Kinehara, Y.H. Ohki, Y. Nakaura, and Y. Fujita. (2003). Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box. Mol. Microbiol. 49: 157-165.

Examples:

TC#NameOrganismal TypeExample
2.A.21.1.1

Pantothenate:Na+ symporter, PanF (Vallari and Rock 1985; Jackowski and Alix 1990; Reizer et al. 1991).

Bacteria

PanF of E. coli

 
Examples:

TC#NameOrganismal TypeExample
2.A.21.2.1

Proline:Na+ symporter, PutP (Jung et al., 2012).  Extracellular loop 4 (eL4) controls periplasmic entry of substrate to the binding site (Raba et al. 2014).  Interactions between the tip of eL4 and the peptide backbone at the end of TMS 10 participate in coordinating conformational alterations underlying the alternating access mechanism of transport (Bracher et al. 2016).  TMS 6 plays a central role in substrate (both Na+ and proline) binding and release on the inner side of the membrane, and functionally relevant amino acids have been identified (Bracher et al. 2016).

Bacteria

PutP of E. coli

 
2.A.21.2.2Sodium/proline symporter (Proline permease)Bacteria

PutP of Staphylococcus aureus

 
2.A.21.2.3

L-proline uptake porter, PutP.  Proline is used via this system as a carbon and nitrogen source.  Induced by proline (Johnson et al. 2008).

Proteobacteria

PutP of Pseudomonas aeruginoas

 
2.A.21.2.4

The high affinity nutritional proline uptake porter, PutP.  PutP is inducible by external (but not internal) proline in a poorly defined process dependent on PutR (Moses et al. 2012). 

Firmicutes

PutP of Bacillus subtilis

 
2.A.21.2.5

Proline uptake porter, OpuE (YerK) (von Blohn et al. 1997).  Regulated by osmotic stress (high osmolarity).  Induction involves σB and σA (Spiegelhalter and Bremer 1998).

Firmicutes

OpuE of Bacillus subtilis

 
2.A.21.2.6

High affinity proline-specific Na+:proline symporter, PutP (Rivera-Ordaz et al. 2013).  Proline is a preferred source of energy for this microaerophilic bacterium.  PutP is efficiently inhibited by the proline analogs, 3,4-dehydro-D,L-proline and L-azetidine-2-carboxylic acid.

Proteobacteria

PutP of Helicobacter pylori

 
Examples:

TC#NameOrganismal TypeExample
2.A.21.3.1

Glucose or galactose:Na+ symporter, SGLT1 (galactose > glucose > fucose). Cotransports water against an osmotic gradient (Naftalin, 2008). TMS IV of the high-affinity sodium-glucose cotransporter participates in sugar binding (Liu et al., 2008).  Also participates in the uptake of resveratrol, an anti atherosclerosis polyphenol (Chen et al. 2013).  hSGLT1 is expressed as a disulfide bridged homodimer via C355; a portion of the intracellular 12-13 loop is re-entrant and readily accessible from the extracellular milieu (Sasseville et al. 2016). Possibly, the extracellular loop between TMS 12 and TMS 13 participates in the sugar transport of SGLT1 (Nagata and Hata 2006). SGLT1 also transports water efficiently. Calculation of the unitary water channel permeability, pf, yielded similar values for cell and proteoliposome experiments. The absence of glucose, Na+, a membrane potential in vesicles, or the directionality of water flow did not grossly altered the pf. Such a weak dependence on protein conformation indicates that a water-impermeable occluded state (glucose and Na+ in their binding pockets) lasts for only a minor fraction of the transport cycle or, alternatively, that occlusion of the substrate does not render the transporter water-impermeable (Erokhova et al. 2016).

Animals

SLC5A1 of Homo sapiens

 
2.A.21.3.10

Na+-dependent, smf-driven, sialic acid transporter, STM1128 (NanP) (Severi et al., 2010). Also transports the related sialic acids, N-glycolylneuraminic acid (Neu5Gc) and 3-keto-3-deoxy-d-glycero-d-galactonononic acid (KDN) (Hopkins et al. 2013). 

Bacteria

STM1128 (NanP) of Salmonella enterica (Q8ZQ35)

 
2.A.21.3.11

The alginate oligosaccharide uptake porter, ToaA (Wargacki et al., 2012).

Bacteria

ToaA in Vibrio splendida (A3UWQ1) 

 
2.A.21.3.12

The alginate oligosaccharide uptake porter, ToaB (Wargacki et al., 2012).

Bacteria

ToaB in Vibrio splendida (A3UWQ9)

 
2.A.21.3.13

The alginate oligosaccharide uptake porter, ToaC (Wargacki et al., 2012).

Bacteria

ToaC in Vibrio splendida (A3UR54)

 
2.A.21.3.14Sodium/myo-inositol cotransporter (Na(+)/myo-inositol cotransporter) (Sodium/myo-inositol transporter 1) (SMIT1) (Solute carrier family 5 member 3)AnimalsSLC5A3 of Homo sapiens
 
2.A.21.3.15

Sodium/glucose cotransporter 5 (Na+/glucose cotransporter 5) (Solute carrier family 5 member 10)

Animals

SLC5A10 of Homo sapiens

 
2.A.21.3.16

Sodium/glucose cotransporter 2 (Na+/glucose cotransporter 2) (Low affinity sodium-glucose cotransporter) (Solute carrier family 5 member 2) of 672 aas and 14 TMSs.  Shows increased expression in human diabetic nephrophathy. Inhibition causes decreased renal lipid accumulation, inflamation and disease symptoms (Wang et al. 2017).

Animals

SLC5A2 of Homo sapiens

 
2.A.21.3.17

Sodium/glucose cotransporter 4 (Na+/glucose cotransporter 4) (hSGLT4) (Solute carrier family 5 member 9).  The involvement of aromatic residue pi interactions, especially with Na+ binding, has been examined (Jiang et al. 2012).

Animals

SLC5A9 of Homo sapiens

 
2.A.21.3.18

Low affinity sodium-glucose cotransporter (Sodium/glucose cotransporter 3) (Na+/glucose cotransporter 3) (Solute carrier family 5 member 4)

Animals

SLC5A4 of Homo sapiens

 
2.A.21.3.19

The putative arabinose porter, AraP (Rodionov D.A., personal communication). Regulated by arabinose regulon AraR.

Bacteroidetes

AraP (Q8AAV7) of Bacteroides thetaiotaomicron

 
2.A.21.3.2

Glucose or galactose:Na+ symporter, SglS or SglT of 543 aas and 14 TMSs (Turk et al. 2006). The 3.0 Å structure is known (Faham et al., 2008). Sodium exit causes a reorientation of transmembrane helix 1 that opens an inner gate required for substrate exit (Watanabe et al., 2010). The involvement of aromatic residue pi interactions, especially with Na+ binding, has been examined (Jiang et al. 2012).

Bacteria

SglS of Vibrio parahaemolyticus

 
2.A.21.3.20

NanT sialic acid transporter of 500 aas (Anba-Mondoloni et al. 2013).

Firmicutes

NanT of Lactobacillus sakei

 
2.A.21.3.21

Putative sugar:sodium symporter of 571 aas and 15 TMSs, YidK

YidK of E. coli

 
2.A.21.3.22

Renal Na+:D-glucose symporter type 1 (Sglt1; Slc5a1) of 662 aas and 14 TMSs.  The distribution in renal tissues has been reported (Althoff et al. 2007). Loop 13, which is associated with phlorizin binding, is variable, as is the interaction with this inhibitor in various species. Immunoreaction was observed in the proximal tubular segments PIa and PIIa, the early distal tubule, and the collecting tubule. Thus, Leucoraja, in contrast to the mammalian kidney, employs only SGLT1 to reabsorb D-glucose in the early, as well as in the late segments of the proximal tubule and probably also in the late distal tubule. It differs from the kidney of the close relative, Squalus acanthias, which uses SGLT2 in more distal proximal tubular segments (Althoff et al. 2007). The ortholog in Squalus acanthias (Spiny dogfish), is 88% identical and has been characterized (Althoff et al. 2006).

Sglt1 of Leucoraja erinacea (Little skate) (Raja erinacea)

 
2.A.21.3.23

Kidney low affinity SGLT (Slc5a1) Na+:D-glucose symporter of 662 aas and 14 TMSs. Of the mammalian homologues, it most resembles SGLT2 (Althoff et al. 2006). 

SGLT of Squalus acanthias (spiny dogfish shark)

 
2.A.21.3.24

Putative Na+:Glucose symporter of 507 aas and 14 TMSs.

Sodium:Glucose symporter of Aeromonas virus 44RR2

 
2.A.21.3.3Nucleoside or glucose(?):Na+ symporter Animals SNST of Oryctolagus cuniculus
 
2.A.21.3.4Glucose:Na+ symporter 3 (low affinity) Animals SAAT1 of Sus scrofa
 
2.A.21.3.5Myoinositol:Na+ symporter, SMIT1 (Aouameur et al., 2007).AnimalsSMIT of Canis familiaris
 
2.A.21.3.6

Myoinositol:Na+ symporter, SMIT2 (also transports D-chiro-inositol, D-glucose and D-xylose) (Coady et al., 2002; Aouameur et al., 2007).  A 5-state model includes cooperative binding of Na+, strong apparent asymmetry of the energy barriers, a rate limiting step which is likely associated with the translocation of the empty transporter, and a turnover rate of 21 s-1 (Sasseville et al. 2014).

Animals

SLC5A11 of Homo sapiens

 
2.A.21.3.7Putative sialic acid uptake permease, NanP (D.A. Rodionov, pers. commun.)BacteriaNanP of Vibrio fischeri (Q5E733)
 
2.A.21.3.8

The putative mannose porter, ManPll (Rodionov et al. 2010).

Proteobacteria

ManPll of Shewanella amazonensis (A1S2A8)

 
2.A.21.3.9

The putative galactose porter, GalPll (Rodionov et al., 2010).

Proteobacteria

GalPll of Shewanella pealeana (A8H019)

 
Examples:

TC#NameOrganismal TypeExample
2.A.21.4.1

The monocarboxylate uptake (H+ symport?) permease, MctP (transports lactate (Km = 4.4 μM), pyruvate (Km = 3.8), propionate, butyrate (butanoic acid), α-hydroxybutyrate, L- and D-alanine (Km = 0.5 mM), and possibly cysteine and histidine) (Hosie et al., 2002).

Bacteria

MctP of Rhizobium leguminosarum

 
2.A.21.4.2

Uncharacterized symporter YodF.  It is regulated by the global transcriptional regulator responding to nitrogen availablity, TnrA, suggesting the YodF transports a nitrogenous compound (Yoshida et al. 2003).

Bacilli

YodF of Bacillus subtilis

 
Examples:

TC#NameOrganismal TypeExample
2.A.21.5.1

Sodium iodide symporter (NIS; also transports other monovalent anions including: ClO3-, SCN-, SeCN-, NO3-, Br-, BF4-, IO4- and BrO3-). It mediates electroneutral active transport of the environmental pollutant perchlorate (Dohan et al., 2007). Five beta-OH group-containing residues (Thr-351, Ser-353, Thr-354, Ser-356, and Thr-357) and Asn-360, all of which putatively face the same side of the helix in TMS IX, plus Asp-369, located in the membrane/cytosol interface, play key roles in NIS function and seem to be involved in Na+ binding/translocation (De la Vieja et al. 2007). Thr-354 is essential for iodide uptake (Tatsumi et al., 2010). The stoichiometry is Na+:I-= 2:1. The G39R mutant (congenital) is inactive. G93 is a pivot for the inwardly to outwardly conformational change (Paroder-Belenitsky et al., 2011).  The protein is present as a dimer (Huc-Brandt et al. 2011).  Functionally equivalent systems have been reviewed (Darrouzet et al. 2014). mutations cause congenital I- transport defects (ITD; Li et al. 2013).  The physiological, medical and mechanistic features of NIS have been reviewed (Portulano et al. 2014).

Animals

SLC5A5 of Homo sapiens

 
2.A.21.5.2

Na+-dependent multivitamin (pantothenate, biotin, lipoate) transporter (de Carvalho and Quick 2011). Broad specificity. May be useful for drug delivery using biotin-conjugated drugs such as Biotin-Acyclovir (B-ACV) (Vadlapudi et al. 2012).  Present in the inclusion membrane that encases Chlamydia trachomatis where it transports vitamins such as biotin (Fisher et al. 2012).  May also take up iodide (de Carvalho and Quick 2011).

Animals

SMVT of Rattus norvegicus

 
2.A.21.5.3

Na+-dependent short chain fatty acid transporter SLC5A8 (tumor suppressor gene product, down-regulated in colon cancer) (substrates: lactate, pyruvate, acetate, propionate, butyrate (Km ≈1 mM)) [propionate:Na+ = 1:3] (Miyauchi et al., 2004). Pyroglutamate (5-oxoproline) is also transported in a Na+- coupled mechanism (Miyauchi et al., 2010). SMCT1 and SMCT2 may transport monocarboxylate drugs (e.g. nicotinate and its derivatives) across the intestinal brush boarder membrane (Gopal et al., 2007; Frank et al. 2008). Wilson et al., 2009 have proposed mechanistic details. SMCT1 can transport urate in a testosterone regulated process (Hosoyamada et al., 2010).  It's phsiological functions have been reviewed (Halestrap 2013). Transports anti-tumor agents, 3-bromopyruvate anddichloroacetate (Su et al. 2016).

Animals

SLC5A8 of Homo sapiens

 
2.A.21.5.4The low affinity (Km (lactate) = 2mM) electroneutral Na+:monocarboxylate (lactate, pyruvate, butyrate, nicotinate) transporter, SMCTn (Plata et al., 2007) AnimalsSMCTn of Danio rerio
(Q7T384)
 
2.A.21.5.5The high affinity (Km (lactate) = 0.2mM) electrogenic Na+ monocarboxylate (lactate, pyruvate, butyrate, nicotinate) transporter, SMCTe (Plata et al., 2007).AnimalsSMCTe of Danio rerio
(Q3ZMH1)
 
2.A.21.5.6Sodium-coupled monocarboxylate transporter 2 (Electroneutral sodium monocarboxylate cotransporter) (Low-affinity sodium-lactate cotransporter) (Solute carrier family 5 member 12)AnimalsSLC5A12 of Homo sapiens
 
2.A.21.5.7

Sodium-dependent multivitamin transporter (Na+-dependent multivitamin transporter) (Solute carrier family 5 member 6)

Animals

SLC5A6 of Homo sapiens

 
2.A.21.5.8

Sodium-coupled transporter, SLC5A11 or cupcake of 600 aas.  A mutant lacking this protein is insensitive to the nutritional value of sugars. It is most similar to mammalian sodium/monocarboxylate co-transporters.  It was prominently expressed in 10-13 pairs of R4 neurons of the ellipsoid body in the brain and functioned in these neurons for selecting appropriate foods (Dus et al. 2013).

Animals

Cupcake of Drosophila melanogaster

 
Examples:

TC#NameOrganismal TypeExample
2.A.21.6.1

Urea active transporter (also transports polyamines; Uemura et al., 2007; Kashiwagi and Igarashi, 2011).

Animals

DUR3 of Saccharomyces cerevisiae

 
2.A.21.6.2

The major transporter for high-affinity urea transport across the plasma membrane of nitrogen-deficient Arabidopsis roots, Dur3 (Kojima et al., 2006; Mérigout et al., 2008). An orthologue of the same function has been characterized in corn (ZmDUR3) (Liu et al. 2014),

Plants

Dur3 of Arabidopsis thaliana (Q9FHJ8)

 
2.A.21.6.3

Rice Dur3 (like 2.A.21.6.2; Wang et al., 2012)

Plants

DUR3 of Oryza sativa (Q7XBS0)

 
2.A.21.6.4

Probable histatin 5 antimicrobial peptide uptake system. May also take up spermidine and be required for morphogenesis (Mayer et al., 2012).

Yeast

Dur31 of Candida albicans (Q59VF2)

 
2.A.21.6.5

Fungal SSS homologue

Fungi

TRP homologue of Neurospora crassa

 
2.A.21.6.6

Urea transporter, UreA of 693 aas.  A three-dimensional model of UreA which, combined with mutagenesis studies, led to the identification of residues important for binding, recognition and translocation of urea, and in the sorting of UreA to the membrane. Residues W82, Y106, A110, T133, N275, D286, Y388, Y437 and S446, located in transmembrane helixes 2, 3, 7 and 11, were found to be involved in the binding, recognition and/or translocation of urea and the sorting of UreA to the membrane. Y106, A110, T133 and Y437 seem to play a role in substrate selectivity, while S446 is necessary for proper sorting of UreA to the membrane (Sanguinetti et al. 2014).

Fungi

UreA of Emericella nidulans (Aspergillus nidulans)

 
Examples:

TC#NameOrganismal TypeExample
2.A.21.7.1Phenylacetate permease, Ppa Bacteria Phenylacetate permease Ppa of Pseudomonas putida
 
2.A.21.7.2

Acetate/glyoxylate permease, ActP (Gimenez et al., 2003).  Also transports tellurite (TeO32-) (Elías et al. 2015).

Bacteria

ActP (YjcG) of E. coli (NP_418491)

 
2.A.21.7.3Pyruvate/acetate/propionate: H+ symporter, MctC (DhlC; cg0953).

Bacteria

MctC of Corynebacterium glutamicum (Q8NS49)

 
2.A.21.7.4

Acetate uptake permease, ActP1; also takes up tellurite (Borghese and Zannoni 2010; Borghese et al. 2011).

Proteobacteria

ActP1 of Rhodobacter capsulatus

 
2.A.21.7.5

Acetate permease ActP-2/ActP2/ActP3 (Borghese and Zannoni 2010; Borghese et al. 2011).  Also takes up tellurite (TeO32-) (Borghese et al. 2016).

Proteobacteria

ActP2 of Rhodobacter capsulatus

 
Examples:

TC#NameOrganismal TypeExample
2.A.21.8.1

High affinity neuronal choline:Na+ symporter, CHT1 (chloride-dependent).  Present in presynaptic terminals of cholinergic neurons.  Has 13 TMSs (Haga 2014).

Animals

CHT1 of Rattus norvegicus

 
2.A.21.8.2

High affinity choline transporter 1 (Hemicholinium-3-sensitive choline transporter) (CHT1) (Solute carrier family 5 member 7).  It is required for synthesis of acetyl choline in cholinergic nerve terminals.  It's 13 TMS topology has been verified with an extracellular N-terminus and an intracellular C-terminus.  It is likely to be a homooligomer (Okuda et al. 2012).  It is defective in hereditary motor neuropathy (Barwick et al. 2012).

Animals

SLC5A7 of Homo sapiens

 
2.A.21.8.3

Putative porter of 436 aas and 13 TMSs

Spirochaetes

Porter of Leptospira biflexa

 
Examples:

TC#NameOrganismal TypeExample
2.A.21.9.1The nitrogen sensor-receptor domain of the CbrA sensor kinaseBacteriaCbrA sensor domain of Pseudomonas aeruginosa
 
2.A.21.9.2The proline sensor-receptor domain of the PrlS sensor kinaseBacteriaPrlS of Aeromonas hydrophila