2.A.72 The K+ Uptake Permease (KUP) Family
Proteins of the KUP/HAK/KT family include the KUP (TrkD) protein of E. coli and homologues in both Gram-positive and Gram-negative bacteria. High affinity (20 μM) K+ uptake systems (Hak1) of the yeast Debaryomyces occidentalis as well as the fungus, Neurospora crassa, and several homologues in plants have been characterized. Arabidopsis thaliana and other plants possess multiple KUP family paralogues. While many plant proteins cluster tightly together, the Hak1 proteins from yeast as well as the two Gram-positive and Gram-negative bacterial proteins are distantly related on the phylogenetic tree for the KUP family. The E. coli protein is 622 amino acyl residues long and has 12 estabilshed transmembrane spanners (440 residues) with a requisite hydrophilic, C-terminal domain of 182 residues, localized to the cytoplasmic side of the membrane (Sato et al. 2014). Deletion of most of the hydrophilic domain reduces but does not abolish KUP transport activity. The function of the C-terminal domain is not known. The E. coli KUP protein is believed to be a secondary transporter. Uptake is blocked by protonophores such as CCCP (but not arsenate), and evidence for a proton symport mechanism has been presented (Zakharyan and Trchounian, 2001). The N. crassa protein was earlier shown to be a K+:H+ symporter, establishing that the KUP family consists of secondary carriers.
The yeast high affinity (KM = 1 µM) K+ transporter Hak1 is 762 amino acyl residues long with 12 putative TMSs. Like the E. coli KUP protein, it possesses a C-terminal hydrophilic domain, probably localized to the cytoplasmic side of the membrane. Hak1 may be able to accumulate K+ 106-fold against a concentration gradient. The plant high and low affinity K+ transporters can complement K+ uptake defects in E. coli. Plant genomes contain large numbers of HAK/KUP/KT transporters, and they show diverse roles in K+ uptake and translocation, salt tolerance and osmotic potential regulation, as well as in controlling root morphology and shoot phenotyping (Li et al. 2018). HAK/KUP/KT transporters can be phosphorylated by CIPK-CBL complexes for activating K+ uptake and probably signaling (Li et al. 2018).
TRK transporters, responsible for the bulk of K+ accumulation in plants, fungi, and bacteria, mediate ion currents driven by the large membrane voltages (-150 to -250 mV) common to non-animal cells. Bacterial TRK proteins resemble K+ channels in their primary sequence, crystallize as membrane dimers having intramolecular K+-channel-like folding, and complex with a cytoplasmic collar formed of four RCK domains (Pardo et al. 2015). Fungal TRK proteins possess a large built-in regulatory domain and a highly conserved pair of transmembrane helices (TMSs 7 and 8, ahead of the C-terminus), postulated to facilitate intramembranal oligomerization.These fungal HAK proteins are chloride channels mediating efflux, a process suppressed by osmoprotective agents. It involve hydrophobic gating and resembles conduction by Cys-loop ligand-gated anion channels. Possibly, the tendency of hydrophobic or amphipathic transmembrane helices to self-organize into oligomers creates novel ionic pathways through membranes: hydrophobic nanopores, pathways of low selectivity governed by the chaotropic behavior of individual ionic species under the influence of membrane voltage (Pardo et al. 2015).
The generalized transport reaction for members of the KUP family is:
K+ (out) + energy → K+ (in).
Low affinity K+ uptake permease, KUP or TrkD. It has 12 established TMSs with four transmembrane acidic residues important for K+ uptake (Sato et al. 2014).
KUP of E. coli (P63183)
Probable potassium transport system protein Kup
Kup of Methanosarcina acetivorans
Probable potassium transport system protein, Kup 1 of 670 aas
Kup1 of Lactococcus lactis
Tiny root hair-1 K+ transporter (TRH1) of 775 aas, also called K+ transporter-3 (POT3; KT3) or KUP4. It regulates root hair elongation (Daras et al. 2015).
TRH1 of Arabidopsis thaliana (Mouse-ear cress)
K+ transporter, HAK1 of 777 aas and 13 TMSs (Guo et al. 2008). Also capable of anion channel activity at high voltages as those found in plants, fungi and bacteria, but not animal cells, possibly using to a new route formed by oligomerization (Pardo et al. 2015).
HAK1 of Nicotiana rustica (Aztec tobacco)
KUP9 (POT9) potassium ion transporter of 807 aas and 14 TMSs. It is up-regluated upon salt stress (Yang et al. 2018).
KUP9 of Arabidopsis thaliana (Mouse-ear cress)
K+:H+ symporter, HAK1. Hak1p is produced at high levels but is down regluated by extracellular K and by expression of Trk1 (2.A.38.2.2) (Rivetta et al. 2013).
Hak1 of Neurospora crassa
Hak1 of Physcomitrella patens (A5PH39)
High affinity K+ transporter, HAKCV (expressed at an early stage during viral infection) (Greiner et al., 2011).
HAKCV of Paramecium bursaria Chlorella virus (A7J6G4)
High affinity potassium transporter 5, HAK5 (Nieves-Cordones et al. 2014). Essential for seed development and postgermination growth in low potassium (Pyo et al. 2010).
Hak5 of Arabidopsis thaliana