8.A.112. The Respiratory Supercomplex Factor 1 (Rcf1) Family
The Saccharomyces cerevisiae respiratory supercomplex factor 1 (Rcf1) protein is located in the mitochondrial inner membrane where it is involved in formation of supercomplexes composed of respiratory complexes III and IV. Zhou et al. 2018 reported the solution structure of Rcf1, which forms a dimer in dodecylphosphocholine (DPC) micelles, where each monomer consists of a bundle of five TMSs and a short flexible soluble helix (SH), although a hydropathy plot shows only two peaks of hydrophobicity. Three TM helices are unusually charged and provide the dimerization interface consisting of 10 putative salt bridges, defining a 'charge zipper' motif. The dimer structure is supported by molecular dynamics (MD) simulations in DPC, although the simulations show a more dynamic dimer interface than suggested by the NMR data. CD and NMR data indicate that Rcf1 undergoes a structural change when reconstituted in liposomes, supported by MD data, suggesting that the dimer structure is unstable in a planar membrane environment, leading to a dynamic monomer-dimer equilibrium. The Rcf1 dimer interacts with cytochrome c, suggesting a role as an electron-transfer bridge between complexes III and IV (Zhou et al. 2018). The N-terminal domain with the two prominent TMSs belongs to the Hypoxia-induced protein conserved region (the Hig-1-N family) while the C-terminal domain is a region of low complexity but seems to be distantly related to a large number of proteins of diverse function. It may be truncated or absent in some homologues. These proteins are found mostly in eukaryotes, but some are from bacteria.
The Saccharomyces cerevisiae respiratory supercomplex factor 1 (Rcf1) protein of 159 aas and 5 TMSs is located in the mitochondrial inner membrane where it is involved in formation of supercomplexes composed of respiratory complexes III and IV (Zhou et al. 2018). See family description for more details.
Rcf1 of Saccharomyces cerevisiae
Rcf1 homolog of 213 aas and 2 predicted TMSs.
Rcf1 of Hypocrea virens (Gliocladium virens) (Trichoderma virens)
Rcf1 homologue with 2 predicted TMSs.
Rcf1 of Tremella mesenterica
Rcf1 homologue of 102 aas and 2 predicted TMSs. The usual C-terminal domain is lacking.
Rcf1 of Drosophila melanogaster (Fruit fly)
HIG1 domain family member 2A, mitochondrial protein of 106 aas and 2 predicted TMSs.
Rcf1 homologue of Homo sapiens
RING-H2 finger protein ATL48 of 349 aas and 3 predicted TMSs. This protein is involved in the pathway protein ubiquitination
Ring-H2 protein of Arabidopsis thaliana (Mouse-ear cress)
Uncharacterized protein of 107 aas and 2 predicted TMSs.
UP of Guillardia theta