9.B.121 The AsmA (AsmA) Family

AsmA mutations were isolated as extragenic suppressors of an OmpF assembly mutant, OmpF315 in E. coli (Deng and Misra, 1996). This suppressor locus encoded a protein that was present in low levels and could only be visualized by Western blotting in cells where AsmA was overproduced. AsmA localized with the inner membrane, but the mutant OmpF assembly step influenced by AsmA occurred in the outer membrane. AsmA null mutations reduced lipopolysaccharide (LPS) levels, thereby lowering the ratios of glycerolphospholipids to LPS and envelope proteins to LPS in the outer membrane. Despite these quantitative alterations, no apparent structural changes in LPS or major phospholipids were noted. Reduced LPS levels in asmA mutants indicated a possible role of AsmA in LPS biogenesis (Deng and Misra, 1996). 



This family belongs to the .

 

References:

Deng, M. and R. Misra. (1996). Examination of AsmA and its effect on the assembly of Escherichia coli outer membrane proteins. Mol. Microbiol. 21: 605-612.

Examples:

TC#NameOrganismal TypeExample
9.B.121.1.1

AsmA (686aas; 2 TMSs at the N- and C-termini)

Bacteria

AsmA of E. coli (P37645)

 
9.B.121.1.2

Putative lipopolysaccharide biogenesis periplasmic protein

Bacteria

LPS biogenesis protein of Gluconaceobacter xylinus (G2I0S0)

 
9.B.121.1.3

Uncharacterized protein, YhjG (675aas; 2 TMSs)

Bacteria

YhjG of Salmonella enterica (G5LV43)

 
Examples:

TC#NameOrganismal TypeExample
9.B.121.2.1

Putative Diguanylate cyclase (1112aas; 2 TMSs)

Bacteria

Diguanylate cyclase of Riftia pachyptila (G2D9D8)

 
Examples:

TC#NameOrganismal TypeExample
Examples:

TC#NameOrganismal TypeExample