9.B.18 The Xanthan Glycosyl Transferase, GumD (GumD) Family

The GumD family cosists of a large number of glycosyltransferases involved in exo-polysaccharide biosynthesis and export.  GumD of Xanthomonas campestris is a costituent of a multicomponent system involved in the biosynthesis and export to the cell surface.  Some of the constituents of this system include GumB (TC# 1.B.18.3.7) and GumC (TC#8.A.3.1.3) (Bianco et al. 2014).  The N-terminus of GumD (residues 30 - 140) consists of a domain of closely packed 4 TMSs, followed by a hydrophilic domain that includes 1 or 2 additional TMSs.  Residues 75 - 275 consist of the NADB_Rossmann or CoA binding_3 domain, while residues 300 to the end consist of the Bacterial Transferase (bac_transf) domain of CDD. 



This family belongs to the .

 

References:

Bianco, M.I., M. Jacobs, S.R. Salinas, A.G. Salvay, M.V. Ielmini, and L. Ielpi. (2014). Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from Xanthomonas campestris. Protein Expr Purif 101: 42-53.

Examples:

TC#NameOrganismal TypeExample
9.B.18.1.1

The glycosyl transferase, GumD, of 484 aas and 5 or 6 TMSs, involved in xanthan gum polysaccharide synthesis and export (Bianco et al. 2014).  The N-terminus consists of a closely packed 4 TMS domain (residues 30 - 140), followed by a hydrophilic domain that includes 1 or 2 additional TMSs.  Residues 75 - 275 consist of the NADB_Rossmann or CoA binding_3 domain, while residues 300 to the end consist of the Bacterial Transferase (bac_transf) domain of CDD.

Proteobacteria

GumD of Xanthomonas campestris

 
9.B.18.1.2

Exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase of 518 aas and 6 TMSs, 4 N-terminal and 2 more centrally located in the large hydrophilic domain.

Actinobacteria

Glycosyltransferase of Microbacterium laevaniformans

 
9.B.18.1.3

Putative UDP-N-acetylgalactosamine-undecaprenyl-phosphate N-acetylgalactosaminephosphotransferase of 453 aas and 7 TMSs.

Putative transferase of Pedobacter glucosidilyticus

 
9.B.18.1.4

UDP-Gal:undecaprenolphosphate Gal-1-P transferase WbaP with 376 aas and 5 TMSs in a 4 + 1 arrangement.

WbaP of E. coli

 
Examples:

TC#NameOrganismal TypeExample
9.B.18.2.1

Putative capsular polysaccharide biosynthetic enzyme, WbpM of 626 aas and 4 N-terminal TMSs.

WbpM of Bdellovibrio bacteriovorus