9.B.29 The small 4-5 TMS Putative Permease (4-5PP) Family

The 4-5PP family consists of proteins from Spirochaetes (Leptospira (subfamily 1)) and many potentially distant homologues in other bacteria (subfamilies 2 - 4) as well as archaea (subfamily 5).  They have 200 - 270 aas with 4 - 5 TMSs. Some of them are encoded adjacent to genes coding for membrane fusion proteins (MFPs; 8.A.1) and outer menbrane factors (OMFs; 1.B.17) implying they are effulx systems. These proteins may be distantly related to but members of the ABC superfamily (3.A.1). 



This family belongs to the .

 

References:

Drew, D., D. Sjöstrand, J. Nilsson, T. Urbig, C.N. Chin, J.W. de Gier, and G. von Heijne. (2002). Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proc. Natl. Acad. Sci. USA 99: 2690-2695.

Examples:

TC#NameOrganismal TypeExample
9.B.29.1.1

Uncharacterized protein of 205 aas and 5 TMSs

Spirochaetes

UP of Leptospira interogans

 
9.B.29.1.2

Uncharacterized protein of 204 aas and 5 TMSs

Spirochaetes

UP of Leptospira biflexa

 
Examples:

TC#NameOrganismal TypeExample
9.B.29.2.1

Uncharacterized protein of 231 aas and 5 TMSs.

Firmicutes

UP of Bacillus sp.

 
9.B.29.2.10

Uncharacterized protein of 227 aas and 5 TMSs

Spirochaetes

UP of Leptospira santarosai

 
9.B.29.2.11

Uncharacterized protein of 198 aas and 5 TMSs

Euryarchaea

UP of Methanocorpusculum labreanum

 
9.B.29.2.12

Uncharacterized putative ABC-type transporter with three constituents, one of 194 aas and 5 TMSs (A3U434),  one encoding another hydrophobic protein of 161 aas and 4 TMSs (A3U435) and another gene encoding an ABC-type ATPase of 251 aas (SufC; A3U437) in the same gene cluster.

Proteobacteria

Putative ABC efflux transporter of Pseudooceanicola batsensis (Oceanicola batsensis)

 
9.B.29.2.13

Uncharacterized protein of the Pfam Yip1 superfamily. 220 aas and 5 TMSs.

Firmicutes

UP of Caldicellulosiruptor saccharolyticus

 
9.B.29.2.14

Uncharacterized protein of 196 aas and 5 TMSs.

Actinobacteria

UP of Rubrobacter xylanophilus

 
9.B.29.2.15

Uncharacterized protein of 221 aas and 5 TMSs.  Also similar to 3.A.1.122.2.

Firmicutes

UP of Carboxydothermus hydrogenoformans

 
9.B.29.2.16

Uncharacterized protein of 267 aas and 5 TMSs with an N-terminal zinc_ribbon_2 domain.

UP of Candidatus Lokiarchaeota archaeon CR_4

 
9.B.29.2.2

Uncharacterized protein of 203 aas and 5 TMSs

Firmicutes

UP of Solibacillus silvestris (Bacillus silvestris)

 
9.B.29.2.3

Uncharacterized protein of 216 aas and 5 TMSs

Firmicutes

UP of Bacillus cereus

 
9.B.29.2.4

Uncharacterized protein of 198 aas and 5 TMSs

Firmicutes

UP of Heliobacterium modesticaldum

 
9.B.29.2.5

Uncharacterized protein of 207 aas and 5 TMSs

Firmicutes

UP of Ammonifex degensii

 
9.B.29.2.6

Uncharacterized protein of 251 aas and 5 TMSs

Firmicutes

UP of Desulfotomaculum carboxydivorans

 
9.B.29.2.7

Uncharacterized protein of 212 aas and 5 TMSs (B8GHI5).  The adjacent gene encodes a homologue of 209 aas and 5 TMSs (B8GHI4; e-41), then a 415 aas protein with 2 TMSs, N- and C-terminal (B8GHI3),  then an ABC ATPase of 234 aas (B8GHI2), then a type 3 membrane ABC protein of 4 TMSs (B8GHI1) and then an inner membrane PrsW type protease (B8GHI0).  B8GHI2 and B8GHI1 appear to comprise an ABC transporter assigned TC# 3.A.1.122.21.

Euryarchaea

UP of Methanosphaerula palustris

 
9.B.29.2.8

Uncharacterized protein of 210 aas and 4 TMSs

Euryarchaea

UP of Methanosarcina barkeri

 
9.B.29.2.9

Uncharacterized protein of 174 aas and 4 TMSs

Euryarchaera

UP of Archaeoglobus fulgidus

 
Examples:

TC#NameOrganismal TypeExample
9.B.29.3.1

Uncharacterized protein of 203 aas and 5 TMSs

Proteobacteria

UP of Pseudomonas fluorescens

 
9.B.29.3.2

Uncharacterized protein of 203 aas and 5 TMSs with UniProt acc# of F9ZP17.  F9ZP16 is a Membrane Fusion Protein (MFP: TC#8.A.1) and F9ZP15 is an outer membrane factor (OMF; TC# 1.B.17). 

Proteobacteria

UP of Acidithiobacillus caldus

 
9.B.29.3.3

Uncharacterized protein of 199 aas and 5 TMSs

Proteobacteria

UP of Aromatoleum aromaticum

 
9.B.29.3.4

Uncharacterized protein of 386 aas and 5 TMSs

Proteobacteria

UP of Sphingobium sp. SYK-6

 
9.B.29.3.5

Uncharacterized protein of 202 aas and 5 TMSs

Proteobacteria

UP of Alkalilimnicola ehrlichii

 
9.B.29.3.6

Uncharacterized protein of 181 aas and 5 TMSs

Bacteroidetes

UP of Bacteroides ovatus

 
Examples:

TC#NameOrganismal TypeExample
9.B.29.4.1

Uncharacterized protein of 191 aas and 5 TMSs

Deinococcus/Thermus

UP of Deinococcus maricopensis

 
Examples:

TC#NameOrganismal TypeExample