9.B.29 The small 4-5 TMS Putative Permease (4-5PP) Family The 4-5PP family consists of proteins from Spirochaetes (Leptospira (subfamily 1)) and many potentially distant homologues in other bacteria (subfamilies 2 - 4) as well as archaea (subfamily 5). They have 200 - 270 aas with 4 - 5 TMSs. Some of them are encoded adjacent to genes coding for membrane fusion proteins (MFPs; 8.A.1) and outer menbrane factors (OMFs; 1.B.17) implying they are effulx systems. These proteins may be distantly related to but members of the ABC superfamily (3.A.1).
This family belongs to the .
References:
Drew, D., D. Sjöstrand, J. Nilsson, T. Urbig, C.N. Chin, J.W. de Gier, and G. von Heijne. (2002). Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proc. Natl. Acad. Sci. USA 99: 2690-2695.
Examples:
TC#	Name	Organismal Type	Example
9.B.29.1.1	Uncharacterized protein of 205 aas and 5 TMSs	Spirochaetes	UP of Leptospira interogans

9.B.29.1.2	Uncharacterized protein of 204 aas and 5 TMSs	Spirochaetes	UP of Leptospira biflexa

Examples:
TC#	Name	Organismal Type	Example
9.B.29.2.1	Uncharacterized protein of 231 aas and 5 TMSs.	Firmicutes	*UP of Bacillus* sp.**

9.B.29.2.10	Uncharacterized protein of 227 aas and 5 TMSs	Spirochaetes	UP of Leptospira santarosai

9.B.29.2.11	Uncharacterized protein of 198 aas and 5 TMSs	Euryarchaea	UP of Methanocorpusculum labreanum

9.B.29.2.12	Uncharacterized putative ABC-type transporter with three constituents, one of 194 aas and 5 TMSs (A3U434), one encoding another hydrophobic protein of 161 aas and 4 TMSs (A3U435) and another gene encoding an ABC-type ATPase of 251 aas (SufC; A3U437) in the same gene cluster.	Proteobacteria	Putative ABC efflux transporter of Pseudooceanicola batsensis (Oceanicola batsensis)

9.B.29.2.13	Uncharacterized protein of the Pfam Yip1 superfamily. 220 aas and 5 TMSs.	Firmicutes	UP of Caldicellulosiruptor saccharolyticus

9.B.29.2.14	Uncharacterized protein of 196 aas and 5 TMSs.	Actinobacteria	UP of Rubrobacter xylanophilus

9.B.29.2.15	Uncharacterized protein of 221 aas and 5 TMSs. Also similar to 3.A.1.122.2.	Firmicutes	UP of Carboxydothermus hydrogenoformans

9.B.29.2.16	Uncharacterized protein of 267 aas and 5 TMSs with an N-terminal zinc_ribbon_2 domain.		UP of Candidatus Lokiarchaeota archaeon CR_4

9.B.29.2.2	Uncharacterized protein of 203 aas and 5 TMSs	Firmicutes	UP of Solibacillus silvestris (Bacillus silvestris)

9.B.29.2.3	Uncharacterized protein of 216 aas and 5 TMSs	Firmicutes	UP of Bacillus cereus

9.B.29.2.4	Uncharacterized protein of 198 aas and 5 TMSs	Firmicutes	UP of Heliobacterium modesticaldum

9.B.29.2.5	Uncharacterized protein of 207 aas and 5 TMSs	Firmicutes	UP of Ammonifex degensii

9.B.29.2.6	Uncharacterized protein of 251 aas and 5 TMSs	Firmicutes	UP of Desulfotomaculum carboxydivorans

9.B.29.2.7	Uncharacterized protein of 212 aas and 5 TMSs (B8GHI5). The adjacent gene encodes a homologue of 209 aas and 5 TMSs (B8GHI4; e-41), then a 415 aas protein with 2 TMSs, N- and C-terminal (B8GHI3), then an ABC ATPase of 234 aas (B8GHI2), then a type 3 membrane ABC protein of 4 TMSs (B8GHI1) and then an inner membrane PrsW type protease (B8GHI0). B8GHI2 and B8GHI1 appear to comprise an ABC transporter assigned TC# 3.A.1.122.21.	Euryarchaea	UP of Methanosphaerula palustris

9.B.29.2.8	Uncharacterized protein of 210 aas and 4 TMSs	Euryarchaea	UP of Methanosarcina barkeri

9.B.29.2.9	Uncharacterized protein of 174 aas and 4 TMSs	Euryarchaera	UP of Archaeoglobus fulgidus

Examples:
TC#	Name	Organismal Type	Example
9.B.29.3.1	Uncharacterized protein of 203 aas and 5 TMSs	Proteobacteria	UP of Pseudomonas fluorescens

9.B.29.3.2	Uncharacterized protein of 203 aas and 5 TMSs with UniProt acc# of F9ZP17. F9ZP16 is a Membrane Fusion Protein (MFP: TC#8.A.1) and F9ZP15 is an outer membrane factor (OMF; TC# 1.B.17).	Proteobacteria	UP of Acidithiobacillus caldus

9.B.29.3.3	Uncharacterized protein of 199 aas and 5 TMSs	Proteobacteria	UP of Aromatoleum aromaticum

9.B.29.3.4	Uncharacterized protein of 386 aas and 5 TMSs	Proteobacteria	UP of Sphingobium sp. SYK-6

9.B.29.3.5	Uncharacterized protein of 202 aas and 5 TMSs	Proteobacteria	UP of Alkalilimnicola ehrlichii

9.B.29.3.6	Uncharacterized protein of 181 aas and 5 TMSs	Bacteroidetes	UP of Bacteroides ovatus

Examples:
TC#	Name	Organismal Type	Example
9.B.29.4.1	Uncharacterized protein of 191 aas and 5 TMSs	*Deinococcus/Thermus*	UP of Deinococcus maricopensis

Examples:
TC#	Name	Organismal Type	Example