9.B.97 The Acyltransferase-3/Putative Acetyl-CoA Transporter (ATAT) Family
The ATAT (COG1835 or Acyltransferase-3) family includes several proteins implicated in both transport of acyl-CoA derivatives and acyl transfer (Moynihan and Clarke, 2010). These proteins have about 330 aas with about 10 putative TMSs in a possible 5+5 arrangement. This family may be distantly related to TC family 9.B.169.
YiaH 10 (5+5?) TMS transmembrane protein (331aas) (The O-acetyltransferase of enterobacterial common antigen (ECA). Exhibits some similarity with TC# 2.A.36.1.7)
YiaH of E. coli K12 (P37669)
Acyltransferase 3 of 371 aas and 10 TMSs
UP of Desulfobulbus propionicus
Putative acyltransferase of 363 aas and 10 TMSs.
Acyltransferase of Slackia exigua
Uncharacterized protein of 369 aas and 10 TMSs.
UP of Clostridium sp. CAG:167
Phage putative 10 TMS, O157 antigen acylating, O-acetyltransferase, Oac. Acetylation blocks superinfection (Perry et al., 2009).
O-antigen transferase, Oac, of E. coli O157 phage ΦV10. (Q286Z2)
The archaeal protein, Msp1344 (10TMSs)
Hypthetical protein, Msp1344 of Methanosphaera stadtmanae (Q2NEN2)
Putative fucose 4-O acetylase (346aas; 10 putative TMSs)
Fucose 4-O acetylase of Vibrio cholerae (C2ITH3)
Acyltransferase 3 of 347 aas and 10 TMSs
Acyltransferase 3 of Thermoanaerobacterium saccharolyticum
Intercellular adhesion protein C of 387 aas and 11 TMSs
Adhesion protein C of Leptospira interrogans
Polysaccharide adhesin export protein of 350 aas and 10 TMSs, IcaC. Involved in polysaccharide intercellular adhesin (PIA) export and biofilm formation (Hennig et al. 2007).
IcaC of Staphylococcus aureus
Uncharacterized protein of 358 aas and 11 putative TMSs.
UP of Leptospira biflexa
Uncharacterized protein of 369 aas and 11 TMSs.
UP of Clostridium cellulovorans
Putative O-acyltransferase homologue (Oac7) (702aas; 14 putative TMSs)
Oac7 of Caenorhabditis elegans (O45283)
Transmembrane acyl transferase of 687 aas and 13 putative TMSs, one at the N-terminus and 12 more together after a hydrophilic domain of about 170 aas, NDG-4. When the gene is mutated or deleted, the life span of C. elegans increases up to 5-fold (Brejning et al. 2014).
NDG-4 of Caenorhabditis elegans
Acyl transferase of 822 aas and 14 putative TMSs. Has an N-terminal TMS followed bya hydrophilic domain of about 270 aas which may include TMSs, followed by about 13 TMSs. Plays a role in the uptake of a range of molecules including lipids and xenobiotic compounds from the intestine to surrounding tissues. Mediates transport of lipids from the intestine to the reproductive tract. Required for efficient yolk transport into oocytes. Vital for embryonic development (Brejning et al. 2014). Mutants in the nrf-6 gene have extended lifespans and increased stress resistance (Brejning et al. 2014).
NRF6 of Caenorhabditis elegans
Putative isovaleryltransferase, MppN of 456aas and 10 putative TMSs.
MppN of Streptomyces hygroscopicus (Q643C2)
Putative O-acetyltransferase, OatA
OatA of Erwinia amylovora (YP_003531188)
Acyltransferase of 437 aas and 12 TMSs.
AT of Nocardia inohanensis
Membrane protein with acyltransferase 3 domain
Acyltransferase of Staphylococcus carnosus
Uncharacterized putative acyl transferase of 389 aas and 10 TMSs
UP of Lentisphaera araneosa
Acyltransferase of 329 aas and 10 TMSs.
Acyltransferase of Erwinia sp. Ejp617
The GumF protein of 364 aas and 10 TMSs (Bianco et al. 2014).
GumF of Xanthomonas campestris
GumG of 356 aas and 9 or 10 TMSs (Bianco et al. 2014).
GumG of Xanthomonas campestris
Uncharacterized protein of 333 aas and 9 TMSs.
UP of Lactobacillus rhamnosus
The succinyl transferase, MdoC, OpgC or YmdD (formerly DUF418), is of 385 aas and 10 TMSs. Osmoregulated periplasmic glucans (OPGs) of E. coli are anionic oligosaccharides that accumulate in the periplasmic space in response to low osmolarity of the medium. Their anionic character is provided by the substitution of the glucosidic backbone by phosphoglycerol originating from membrane phospholipids and by succinyl residues from an unknown origin. The mdoC gene is within the mdoGH operon necessary for the synthesis of the OPG backbone (Lacroix et al. 1999). The same is true for Rhodobacter spheroides in which in correspondng operon is the opgGIHC operon (Cogez et al. 2002).
MdoC of E. coli
Acyltransferase of 239 aas and 6 TMSs.
Acyltransferase of Leptospira interrogans
MdoC homologue of 366 aas and 10 TMSs.
MdoC of Clostridium uliginosum
OpgC homologue of 351 aas and 10 TMSs in a 3 + 4 + 3 arrangment.
OpgC homolgue of Haemophilus haemolyticus
Uncharacterized acyl transferase of 366 aas and 10 TMSs
UP of Zobellia galactanivorans
Uncharacterized acyl transferase of 336 aas and 10 TMSs.
UP of Staphylococcus aureus
Uncharacterized acyltransferase of 409 aas ahd 10 TMSs
UP of Nocardia flavorosea
Succinyl transferase, OpgC or MdoC (formerly DUF418) of 399 aas and 10 TMSs. It succinylates the osmotically-induced periplasmic oligoglucan (OPG) and is encoded by a gene downstream of the OPG-encoding genes, (opgGIH) (Cogez et al. 2002).
ObgC of Rhodobacter sphaeroides
OpgC homologue of 397 aas and 10 TMSs in a 3 + 4 + 3 arrangement.
OpgC of Aureimonas altamirensis
OpgC homologue of 353 aas and 10 TMSs in a 3 + 4 + 3 arrangement.
OpgC homolgue of Candidatus Saccharibacteria bacterium
OpgC of 397 aas and 10 TMSs in a 3 + 4 + 3 arrangement.
OpgC of Klebsiella pneumoniae
OpgC homologue of 388 aas and 9 TMSs in a 2 + 4 + 3 arrangement. The N-terminal TMS may be missing because of an incorrect initiation codon assignment.
OpgC of Gordonia phthalatica