9.B.97 The Acyltransferase-3/Putative Acetyl-CoA Transporter (ATAT) Family

The ATAT (COG1835 or Acyltransferase-3) family includes several proteins implicated in both transport of acyl-CoA derivatives and acyl transfer (Moynihan and Clarke, 2010). These proteins have about 330 aas with about 10 putative TMSs in a possible 5+5 arrangement. This family may be distantly related to TC family 9.B.169.



This family belongs to the Transmembrane Acyl Transferease (TmAT) Superfamily.

 

References:

Bianco, M.I., M. Jacobs, S.R. Salinas, A.G. Salvay, M.V. Ielmini, and L. Ielpi. (2014). Biophysical characterization of the outer membrane polysaccharide export protein and the polysaccharide co-polymerase protein from Xanthomonas campestris. Protein Expr Purif 101: 42-53.

Brejning, J., S. Nørgaard, L. Schøler, T.H. Morthorst, H. Jakobsen, G.J. Lithgow, L.T. Jensen, and A. Olsen. (2014). Loss of NDG-4 extends lifespan and stress resistance in Caenorhabditis elegans. Aging Cell 13: 156-164.

Cogez, V., E. Gak, A. Puskas, S. Kaplan, and J.P. Bohin. (2002). The opgGIH and opgC genes of Rhodobacter sphaeroides form an operon that controls backbone synthesis and succinylation of osmoregulated periplasmic glucans. Eur J Biochem 269: 2473-2484.

Hennig, S., S. Nyunt Wai, and W. Ziebuhr. (2007). Spontaneous switch to PIA-independent biofilm formation in an ica-positive Staphylococcus epidermidis isolate. Int. J. Med. Microbiol. 297: 117-122.

Lacroix, J.M., E. Lanfroy, V. Cogez, Y. Lequette, A. Bohin, and J.P. Bohin. (1999). The mdoC gene of Escherichia coli encodes a membrane protein that is required for succinylation of osmoregulated periplasmic glucans. J. Bacteriol. 181: 3626-3631.

Moynihan, P.J. and A.J. Clarke. (2010). O-acetylation of peptidoglycan in gram-negative bacteria: identification and characterization of peptidoglycan O-acetyltransferase in Neisseria gonorrhoeae. J. Biol. Chem. 285: 13264-13273.

Perry, L.L., P. SanMiguel, U. Minocha, A.I. Terekhov, M.L. Shroyer, L.A. Farris, N. Bright, B.L. Reuhs, and B.M. Applegate. (2009). Sequence analysis of Escherichia coli O157:H7 bacteriophage PhiV10 and identification of a phage-encoded immunity protein that modifies the O157 antigen. FEMS Microbiol. Lett. 292: 182-186.

Examples:

TC#NameOrganismal TypeExample
9.B.97.1.1

YiaH 10 (5+5?) TMS transmembrane protein (331aas) (The O-acetyltransferase of enterobacterial common antigen (ECA). Exhibits some similarity with TC# 2.A.36.1.7)

Bacteria

YiaH of E. coli K12 (P37669)

 
9.B.97.1.10

Acyltransferase 3 of 371 aas and 10 TMSs

Proteobacteria

UP of Desulfobulbus propionicus

 
9.B.97.1.11

Putative acyltransferase of 363 aas and 10 TMSs.

Acyltransferase of Slackia exigua

 
9.B.97.1.12

Uncharacterized protein of 369 aas and 10 TMSs.

UP of Clostridium sp. CAG:167

 
9.B.97.1.2

Phage putative 10 TMS, O157 antigen acylating, O-acetyltransferase, Oac. Acetylation blocks superinfection (Perry et al., 2009).

Virus

O-antigen transferase, Oac, of E. coli O157 phage ΦV10. (Q286Z2)

 
9.B.97.1.3

The archaeal protein, Msp1344 (10TMSs)

Archaea

Hypthetical protein, Msp1344 of Methanosphaera stadtmanae (Q2NEN2)

 

 
9.B.97.1.4

Putative fucose 4-O acetylase (346aas; 10 putative TMSs)

Bacteria

Fucose 4-O acetylase of Vibrio cholerae (C2ITH3)

 
9.B.97.1.5

Acyltransferase 3 of 347 aas and 10 TMSs

Firmicutes

Acyltransferase 3 of Thermoanaerobacterium saccharolyticum

 
9.B.97.1.6

Intercellular adhesion protein C of 387 aas and 11 TMSs

Spirochaetes

Adhesion protein C of Leptospira interrogans

 
9.B.97.1.7

Polysaccharide adhesin export protein of 350 aas and 10 TMSs, IcaC.  Involved in polysaccharide intercellular adhesin (PIA) export and biofilm formation (Hennig et al. 2007).

Firmicutes

IcaC of Staphylococcus aureus

 
9.B.97.1.8

Uncharacterized protein of 358 aas and 11 putative TMSs.

Spirochaetes

UP of Leptospira biflexa

 
9.B.97.1.9

Uncharacterized protein of 369 aas and 11 TMSs.

Firmicutes

UP of Clostridium cellulovorans

 
Examples:

TC#NameOrganismal TypeExample
9.B.97.2.1

Putative O-acyltransferase homologue (Oac7) (702aas; 14 putative TMSs)

Eukaryotes

Oac7 of Caenorhabditis elegans (O45283)

 
9.B.97.2.2

Transmembrane acyl transferase of 687 aas and 13 putative TMSs, one at the N-terminus and 12 more together after a hydrophilic domain of about 170 aas, NDG-4.  When the gene is  mutated or deleted, the life span of C. elegans increases up to 5-fold (Brejning et al. 2014).

Animals

NDG-4 of Caenorhabditis elegans

 
9.B.97.2.3

Acyl transferase of 822 aas and 14 putative TMSs.  Has an N-terminal TMS followed bya hydrophilic domain of about 270 aas which may include TMSs, followed by about 13 TMSs.  Plays a role in the uptake of a range of molecules including lipids and xenobiotic compounds from the intestine to surrounding tissues. Mediates transport of lipids from the intestine to the reproductive tract. Required for efficient yolk transport into oocytes. Vital for embryonic development (Brejning et al. 2014).  Mutants in the nrf-6 gene have extended lifespans and increased stress resistance (Brejning et al. 2014).

Animals

NRF6 of Caenorhabditis elegans

 
Examples:

TC#NameOrganismal TypeExample
9.B.97.3.1

Putative isovaleryltransferase, MppN of 456aas and 10 putative TMSs.

Bacteria

MppN of Streptomyces hygroscopicus (Q643C2)

 
Examples:

TC#NameOrganismal TypeExample
9.B.97.4.1

Putative O-acetyltransferase, OatA

Bacteria

OatA of Erwinia amylovora (YP_003531188)

 
9.B.97.4.2

Acyltransferase of 437 aas and 12 TMSs.

AT of Nocardia inohanensis

 
Examples:

TC#NameOrganismal TypeExample
9.B.97.5.1

Membrane protein with acyltransferase 3 domain

Firmicutes

Acyltransferase of Staphylococcus carnosus

 
9.B.97.5.2

Uncharacterized putative acyl transferase of 389 aas and 10 TMSs

UP of Lentisphaera araneosa

 
9.B.97.5.3

Acyltransferase of 329 aas and 10 TMSs.

Acyltransferase of Erwinia sp. Ejp617

 
Examples:

TC#NameOrganismal TypeExample
9.B.97.6.1

The GumF protein of 364 aas and 10 TMSs (Bianco et al. 2014).

Proteobacteria

GumF of Xanthomonas campestris

 
9.B.97.6.2

GumG of 356 aas and 9 or 10 TMSs (Bianco et al. 2014).

Proteobacteria

GumG of Xanthomonas campestris

 
9.B.97.6.3

Uncharacterized protein of 333 aas and 9 TMSs.

UP of Lactobacillus rhamnosus

 
Examples:

TC#NameOrganismal TypeExample
9.B.97.7.1

The succinyl transferase, MdoC, OpgC or YmdD (formerly DUF418), is of 385 aas and 10 TMSs. Osmoregulated periplasmic glucans (OPGs) of E. coli are anionic oligosaccharides that accumulate in the periplasmic space in response to low osmolarity of the medium. Their anionic character is provided by the substitution of the glucosidic backbone by phosphoglycerol originating from  membrane phospholipids and by succinyl residues from an unknown origin. The mdoC gene is within the mdoGH operon necessary for the synthesis of the OPG backbone (Lacroix et al. 1999). The same is true for Rhodobacter spheroides in which in correspondng operon is the opgGIHC operon (Cogez et al. 2002).

MdoC of E. coli

 
9.B.97.7.2

Acyltransferase of 239 aas and 6 TMSs.

Acyltransferase of Leptospira interrogans

 
9.B.97.7.3

MdoC homologue of 366 aas and 10 TMSs.

MdoC of Clostridium uliginosum

 
9.B.97.7.4

OpgC homologue of 351 aas and 10 TMSs in a 3 + 4 + 3 arrangment.

OpgC homolgue of Haemophilus haemolyticus

 
9.B.97.7.5

Uncharacterized acyl transferase of 366 aas and 10 TMSs

UP of Zobellia galactanivorans

 
9.B.97.7.6

Uncharacterized acyl transferase of 336 aas and 10 TMSs.

UP of Staphylococcus aureus

 
9.B.97.7.7

Uncharacterized acyltransferase of 409 aas ahd 10 TMSs

UP of Nocardia flavorosea

 
Examples:

TC#NameOrganismal TypeExample
9.B.97.8.1

Succinyl transferase, OpgC or MdoC (formerly DUF418) of 399 aas and 10 TMSs.  It succinylates the osmotically-induced periplasmic oligoglucan (OPG) and is encoded by a gene downstream of the OPG-encoding genes, (opgGIH) (Cogez et al. 2002).

ObgC of Rhodobacter sphaeroides

 
9.B.97.8.2

OpgC homologue of 397 aas and 10 TMSs in a 3 + 4 + 3 arrangement.

OpgC of Aureimonas altamirensis

 
9.B.97.8.3

OpgC homologue of 353 aas and 10 TMSs in a 3 + 4 + 3 arrangement.

OpgC homolgue of Candidatus Saccharibacteria bacterium

 
9.B.97.8.4

OpgC of 397 aas and 10 TMSs in a 3 + 4 + 3 arrangement.

OpgC of Klebsiella pneumoniae

 
9.B.97.8.5

OpgC homologue of 388 aas and 9 TMSs in a 2 + 4 + 3 arrangement. The N-terminal TMS may be missing because of an incorrect initiation codon assignment.

OpgC of Gordonia phthalatica

 
Examples:

TC#NameOrganismal TypeExample