TCDB is operated by the Saier Lab Bioinformatics Group
Transporter Information:
Name: solute carrier family 6 (neurotransmitter transporter), member 14
Symbol: SLC6A14
TC: 2.A.22.2.3
Locations: Xq23-q24
GenBank: AF151978
Swiss-Prot: Q9UN76
Accession Number: NM_007231
PubMed (10446133): Sloan JL, Mager S. Cloning and functional expression of a human Na(+) and Cl(-)-dependent neutraland cationic amino acid transporter B(0+).J Biol Chem. 1999 Aug 20;274(34):23740-5. PMID: 10446133 [PubMed - indexed for MEDLINE]

A Na(+)-dependent neutral and cationic amino acid transport system (B(0+)) plays an important role in many cells and tissues; however, the molecular basis for this transport system is still unknown. To identify new transporters, the expressed sequence tag database was queried, and cDNA fragments with sequence similarity to the Na(+)/Cl(-)-dependent neurotransmitter transporter family were identified. Based on these sequences, rapid amplification of cDNA ends of human mammary gland cDNA was used to obtain a cDNA of 4.5 kilobases (kb). The open reading frame encodes a 642-amino acid protein named amino acid transporter B(0+). Human ATB(0+) (hATB(0+)) is a novel member of the Na(+)/Cl(-)-dependent neurotransmitter transporter family with the highest sequence similarity to the glycine and proline transporters. Northern blot analysis identified transcripts of approximately 4.5 kb and approximately 2 kb in the lung. Another tissue survey suggests expression in the trachea, salivary gland, mammary gland, stomach, and pituitary gland. Electrophysiology and radiolabeled amino acid uptake measurements were used to functionally characterize the transporter expressed in Xenopus oocytes. hATB(0+) was found to transport both neutral and cationic amino acids, with the highest affinity for hydrophobic amino acids and the lowest affinity for proline. Amino acid transport was Na(+) and Cl(-)-dependent and was attenuated in the presence of 2-aminobicyclo-[2.2.1]-heptane-2-carboxylic acid, a system B(0+) inhibitor. These characteristics are consistent with system B(0+) amino acid transport. Thus, hATB(0+) is the first cloned B(0+) amino acid transporter.

>tr|Q9UN76 AMINO ACID TRANSPORTER B0+ - Homo sapiens (Human).