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2.A.7.1.6
The heterooligomeric drug resistance efflux pump, EbrAB (substrates: ethidium bromide, acriflavin, pyronin Y, and safranin O) (Zhang et al., 2007).

Accession Number:O31792
Protein Name:EbrA
Length:105
Molecular Weight:11349.00
Species:Bacillus subtilis [1423]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate safranin O, 3,6-diamino-10-methylacridinium chloride, pyronin Y, ethidium bromide

Cross database links:

RefSeq: NP_389612.1   
Entrez Gene ID: 940059   
Pfam: PF00893   
KEGG: bsu:BSU17300   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0006810 P:transport

References (2)

[1] “A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis.”  Masaoka Y.et.al.   10735876
[2] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MLIGYIFLTI AICSESIGAA MLKVSDGFKK WKPSALVVIG YSLAFYMLSL TLNHIPLSLS 
61:	YATWSGAGTV LTTVIGVKWF KEDLNAKGLI GILLLLSGVV LLNWP