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3.A.5.9.1
Sec-SRP translocase complex. The BAP29 and BAP31 (also called BCAP31) proteins interact directly with the Sec translocon (Wilson & Barlowe et al., 2010).  SRP68 and SRP72 form a complex with SRP RNA and SRP19.  The SRP68 binding site for the RNA is a tetratricopeptide-like module that bends the RNA and inserts an arginine-rich helix into the major groove to open the conserved 5f RNA loop and remodel the RNA for protein translocation (Grotwinkel et al. 2014).  Sec31 (Sec 31L1; HSPC334; HSPC275) is an outer cage component of the coat protein complex II (COPII) machinery which is recruited to specialized regions of the ER, called ER exit sites (ERES), where it plays a central role in the early secretory pathway. Sec31 also interacts with ALG-2 (Programed cell death protein 6 (PDCD6)) and annexin A11 (AnxA11) (Shibata et al. 2015). The Sec61 translocon mediates poorly efficient membrane insertion of Arg-containing TMSs, but a combination of arginine snorkeling, bilayer deformation, and peptide tilting is sufficient to lower the penalty of Arg insertion to an extent that a hydrophobic TMS with a central Arg residue readily inserts into a membrane (Ulmschneider et al. 2017). Mycolactone is a bacterium-derived macrolide that blocks the biogenesis of a large array of secretory and integral transmembrane proteins through potent inhibition of the Sec61 translocon (Morel et al. 2018). The Sec61α subunit possesses an opening between TMS2b and TMS7, the lateral gate, that is the exit for signal sequences and TMSs of translocating polypeptides to the lipid bilayer (Kida and Sakaguchi 2018). BCAP31 (BAP31; 246 aas and 3 N-terminal TMSs) is an ER chaparone that plays a role in the export of secreted proteins in the ER as well as the recognition of abnormally folded protein for targeting to the ER associated-degradation (ERAD) pathway (Wakana et al. 2008). It also serves as a cargo receptor for the export of transmembrane proteins (Annaert et al. 1997). Sec61 is the target of the cytotoxic plant-derived compound, ipomoeassin F (see TC family 8.C.10).

Accession Number:O94979
Protein Name:Protein transport protein Sec31A
Length:1220
Molecular Weight:133015.00
Species:Homo sapiens (Human) [9606]
Location1 / Topology2 / Orientation3: Cytoplasm1
Substrate protein polypeptide chain

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Structure:
3WXA     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKLKEVDRTA MQAWSPAQNH PIYLATGTSA QQLDATFSTN ASLEIFELDL SDPSLDMKSC 
61:	ATFSSSHRYH KLIWGPYKMD SKGDVSGVLI AGGENGNIIL YDPSKIIAGD KEVVIAQNDK 
121:	HTGPVRALDV NIFQTNLVAS GANESEIYIW DLNNFATPMT PGAKTQPPED ISCIAWNRQV 
181:	QHILASASPS GRATVWDLRK NEPIIKVSDH SNRMHCSGLA WHPDVATQMV LASEDDRLPV 
241:	IQMWDLRFAS SPLRVLENHA RGILAIAWSM ADPELLLSCG KDAKILCSNP NTGEVLYELP 
301:	TNTQWCFDIQ WCPRNPAVLS AASFDGRISV YSIMGGSTDG LRQKQVDKLS SSFGNLDPFG 
361:	TGQPLPPLQI PQQTAQHSIV LPLKKPPKWI RRPVGASFSF GGKLVTFENV RMPSHQGAEQ 
421:	QQQQHHVFIS QVVTEKEFLS RSDQLQQAVQ SQGFINYCQK KIDASQTEFE KNVWSFLKVN 
481:	FEDDSRGKYL ELLGYRKEDL GKKIALALNK VDGANVALKD SDQVAQSDGE ESPAAEEQLL 
541:	GEHIKEEKEE SEFLPSSGGT FNISVSGDID GLITQALLTG NFESAVDLCL HDNRMADAII 
601:	LAIAGGQELL ARTQKKYFAK SQSKITRLIT AVVMKNWKEI VESCDLKNWR EALAAVLTYA 
661:	KPDEFSALCD LLGTRLENEG DSLLQTQACL CYICAGNVEK LVACWTKAQD GSHPLSLQDL 
721:	IEKVVILRKA VQLTQAMDTS TVGVLLAAKM SQYANLLAAQ GSIAAALAFL PDNTNQPNIM 
781:	QLRDRLCRAQ GEPVAGHESP KIPYEKQQLP KGRPGPVAGH HQMPRVQTQQ YYPHGENPPP 
841:	PGFIMHGNVN PNAAGQLPTS PGHMHTQVPP YPQPQPYQPA QPYPFGTGGS AMYRPQQPVA 
901:	PPTSNAYPNT PYISSASSYT GQSQLYAAQH QASSPTSSPA TSFPPPPSSG ASFQHGGPGA 
961:	PPSSSAYALP PGTTGTLPAA SELPASQRTG PQNGWNDPPA LNRVPKKKKM PENFMPPVPI 
1021:	TSPIMNPLGD PQSQMLQQQP SAPVPLSSQS SFPQPHLPGG QPFHGVQQPL GQTGMPPSFS 
1081:	KPNIEGAPGA PIGNTFQHVQ SLPTKKITKK PIPDEHLILK TTFEDLIQRC LSSATDPQTK 
1141:	RKLDDASKRL EFLYDKLREQ TLSPTITSGL HNIARSIETR NYSEGLTMHT HIVSTSNFSE 
1201:	TSAFMPVLKV VLTQANKLGV