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1.C.36.3.1
IIITCP protein complex, IpaB/IpaC/IpaD. Physical contact with host cells initiates secretion and leads to assembly of a pore, IpaB/IpaC, in the host cell membrane. The active needle tip complex of S. flexneri is composed of a tip protein, IpaD, and the two pore-forming proteins, IpaB and IpaC. The atomic structures of IpaD and a protease-stable coiled-coil fragment in the N-terminal regions of IpaB from S. flexneri and the homologous SipB from Salmonella enterica have been determined (Barta et al. 2012).  Structural comparisons revealed similarity to the coiled-coil regions of pore-forming proteins such as colicin Ia (TC# 1.C.1.1.1).  Interaction between IpaB and IpaD at the needle tip is key to host cell sensing, orchestration of IpaC secretion and its subsequent assembly at needle tips (Veenendaal et al. 2007). The N-terminus of IpaC is extracellular and the C-terminus is intracellular, and its topology has been studied (Russo et al. 2019). Residures lining the pore channel of the plasma membrane-embedded Shigella flexneri type 3 secretion translocase, IpaB, have been identified (Chen et al. 2021).

Accession Number:P18013
Protein Name:IpaD
Length:332
Molecular Weight:36639.00
Species:Shigella flexneri [623]
Location1 / Topology2 / Orientation3: Secreted1
Substrate

Cross database links:

RefSeq: NP_085288.1    NP_858259.1   
Entrez Gene ID: 1238053    876444   
Pfam: PF06511   
KEGG: sfl:CP0126   

Gene Ontology

GO:0005576 C:extracellular region
GO:0009405 P:pathogenesis

References (10)

[1] “Characterization of invasion plasmid antigen genes (ipaBCD) from Shigella flexneri.”  Venkatesan M.M.et.al.   3057506
[2] “Functional organization and nucleotide sequence of virulence region-2 on the large virulence plasmid in Shigella flexneri 2a.”  Sasakawa C.et.al.   2552264
[3] “The virulence plasmid pWR100 and the repertoire of proteins secreted by the type III secretion apparatus of Shigella flexneri.”  Buchrieser C.et.al.   11115111
[4] “Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri.”  Venkatesan M.M.et.al.   11292750
[5] “Comparison of two major forms of the Shigella virulence plasmid pINV: positive selection is a major force driving the divergence.”  Lan R.et.al.   14573649
[6] “Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157.”  Jin Q.et.al.   12384590
[7] “Nucleotide sequence of the invasion plasmid antigen B and C genes (ipaB and ipaC) of Shigella flexneri.”  Baudry B.et.al.   3071655
[8] “The secretion of the Shigella flexneri Ipa invasins is activated by epithelial cells and controlled by IpaB and IpaD.”  Menard R.et.al.   7957095
[9] “IpaD of Shigella flexneri is independently required for regulation of Ipa protein secretion and efficient insertion of IpaB and IpaC into host membranes.”  Picking W.L.et.al.   15731041
[10] “Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD.”  Johnson S.et.al.   17077085
Structure:
2J0N   2J0O   2JAA   3R9V   4D3E   5VXJ   5VXK   5VXL   5VXM      [...more]

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNITTLTNSI STSSFSPNNT NGSSTETVNS DIKTTTSSHP VSSLTMLNDT LHNIRTTNQA 
61:	LKKELSQKTL TKTSLEEIAL HSSQISMDVN KSAQLLDILS RNEYPINKDA RELLHSAPKE 
121:	AELDGDQMIS HRELWAKIAN SINDINEQYL KVYEHAVSSY TQMYQDFSAV LSSLAGWISP 
181:	GGNDGNSVKL QVNSLKKALE ELKEKYKDKP LYPANNTVSQ EQANKWLTEL GGTIGKVSQK 
241:	NGGYVVSINM TPIDNMLKSL DNLGGNGEVV LDNAKYQAWN AGFSAEDETM KNNLQTLVQK 
301:	YSNANSIFDN LVKVLSSTIS SCTDTDKLFL HF