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Sec-SRP translocase complex. The BAP29 and BAP31 (also called BCAP31) proteins interact directly with the Sec translocon (Wilson & Barlowe et al., 2010).  SRP68 and SRP72 form a complex with SRP RNA and SRP19.  The SRP68 binding site for the RNA is a tetratricopeptide-like module that bends the RNA and inserts an arginine-rich helix into the major groove to open the conserved 5f RNA loop and remodel the RNA for protein translocation (Grotwinkel et al. 2014).  Sec31 (Sec 31L1; HSPC334; HSPC275) is an outer cage component of the coat protein complex II (COPII) machinery which is recruited to specialized regions of the ER, called ER exit sites (ERES), where it plays a central role in the early secretory pathway. Sec31 also interacts with ALG-2 (Programed cell death protein 6 (PDCD6)) and annexin A11 (AnxA11) (Shibata et al. 2015). The Sec61 translocon mediates poorly efficient membrane insertion of Arg-containing TMSs, but a combination of arginine snorkeling, bilayer deformation, and peptide tilting is sufficient to lower the penalty of Arg insertion to an extent that a hydrophobic TMS with a central Arg residue readily inserts into a membrane (Ulmschneider et al. 2017). Mycolactone is a bacterium-derived macrolide that blocks the biogenesis of a large array of secretory and integral transmembrane proteins through potent inhibition of the Sec61 translocon (Morel et al. 2018). The Sec61α subunit possesses an opening between TMS2b and TMS7, the lateral gate, that is the exit for signal sequences and TMSs of translocating polypeptides to the lipid bilayer (Kida and Sakaguchi 2018). BCAP31 (BAP31; 246 aas and 3 N-terminal TMSs) is an ER chaparone that plays a role in the export of secreted proteins in the ER as well as the recognition of abnormally folded protein for targeting to the ER associated-degradation (ERAD) pathway (Wakana et al. 2008). It also serves as a cargo receptor for the export of transmembrane proteins (Annaert et al. 1997). Sec61 is the target of the cytotoxic plant-derived compound, ipomoeassin F (see TC family 8.C.10).

Accession Number:P60468
Protein Name:SEC61 beta subunit
Molecular Weight:9974.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Endoplasmic reticulum membrane1 / Single-pass membrane protein2
Substrate protein polypeptide chain

Cross database links:

RefSeq: NP_006799.1   
Entrez Gene ID: 10952   
Pfam: PF03911   
OMIM: 609214  gene
KEGG: hsa:10952   

Gene Ontology

GO:0031205 C:endoplasmic reticulum Sec complex
GO:0016021 C:integral to membrane
GO:0048408 F:epidermal growth factor binding
GO:0030433 P:ER-associated protein catabolic process
GO:0000060 P:protein import into nucleus, translocation
GO:0030970 P:retrograde protein transport, ER to cytosol
GO:0055085 P:transmembrane transport

References (11)

[1] “Evolutionary conservation of components of the protein translocation complex.”  Hartmann   8107851
[2] “DNA sequence and analysis of human chromosome 9.”  Humphray   15164053
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project   15489334
[4] “Mammalian Sec61 is associated with Sec62 and Sec63.”  Meyer   10799540
[5] “Global phosphoproteome of HT-29 human colon adenocarcinoma cells.”  Kim   16083285
[6] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.”  Olsen   17081983
[7] “Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.”  Yu   17924679
[8] “Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry.”  Molina   17287340
[9] “A quantitative atlas of mitotic phosphorylation.”  Dephoure   18669648
[10] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci   19413330
[11] “Large-scale proteomics analysis of the human kinome.”  Oppermann   19369195
4cg5   6W6L     

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