Search TCDB: (?)
TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.7.1.8
The putative heterodimeric SMR efflux pump, NepAB, encoded in a nicotine degradation plasmid, pAO1 (Baitsch et al., 2001; Brandsch, 2006); [probably exports methylamine; may also export excess nicotine, methylamine and/or the intermediate of nicotine catabolism, N-methyl-aminobutyrate] (Ganas et al. 2007). Uptake (Km=6μM) occurs by facilitated diffusion (Ganas and Brandsch, 2009).

Accession Number:Q8GAI5
Protein Name:Putative multidrug resistance efflux protein (NepA)
Length:116
Molecular Weight:12440.00
Species:Arthrobacter nicotinovorans [29320]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate methylamine, (S)-nicotine

Cross database links:

Pfam: PF00893   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0006810 P:transport

References (7)

[1] “Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans.”  Grether-Beck S.et.al.   7815950
[2] “A pAO1-encoded molybdopterin cofactor gene (moaA) of Arthrobacter nicotinovorans: characterization and site-directed mutagenesis of the encoded protein.”  Menendez C.et.al.   8588735
[3] “Molybdate-uptake genes and molybdopterin-biosynthesis genes on a bacterial plasmid: characterization of MoeA as a filament-forming protein with adenosinetriphosphatase activity.”  Menendez C.et.al.   9428706
[4] “Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans.”  Schenk S.et.al.   9878353
[5] “IS1473, a putative insertion sequence identified in the plasmid pAO1 from Arthrobacter nicotinovorans: isolation, characterization, and distribution among Arthrobacter species.”  Menendez C.et.al.   9073580
[6] “Gene cluster on pAO1 of Arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine: cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydroxylase.”  Baitsch D.et.al.   11514508
[7] “Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter nicotinovorans and identification of a pAO1-dependent nicotine uptake system.”  Igloi G.L.et.al.   12618462

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MQTRYGRRAL TIWPLLLLAI AAEVAATSLL PQTNGFRKLK PTVAVACLYT VAFALLAQIL 
61:	KFTDIGIAYA LWAGLGTASV AVIGVLFRNE RFSWKHAIGL ALVVTGVVTL NLQAGQ