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1.A.120.1.1
The coronaviral  double membrane-spanning pore complex in the replication organelle (RO) of the viral double membrane vesicle (DMV). The pore consists of a hexameric complex. The primary constituent of each of the six subunits of the pore is the non-structural protein-3, nsp3, of 2309 aas, which may have 5 TMSs, based on a hydrophathy plot of the protein. These are clustered together about two-thrids of the way towards the C-terminus. It is the core constituent of the pore and has both its N-terminal domain of 160 kDa and a smaller C-terminal domain, which contains a ubiquitin domain (Ubi1) of 12.6 kDa, that together form the prongs of the cytoplasmic crown. nsp3 interacts with nsp4, and this complex is believed to drive membrane pairing and participate in DMV biogenesis (Wolff et al. 2020). It may associate with other viral and host proteins.

Accession Number:YP_009915690.1
Protein Name:YP_009915690.1 nsp3 [Murine hepatitis virus]
Length:2310
Molecular Weight:
Species:Murine hepatitis virus [11138]
Number of TMSs:5
Substrate (ribonucleotide)n+m

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	GKKVEFNDKP KVRKIPSTRK IKITFALDAT FDSVLSKACS EFEVDKDVTL DELLDVVLDA 
61:	VESTLSPCKE HDVIGTKVCA LLDRLAGDYV YLFDEGGDEV IAPRMYCSFS APDDEDCVAA 
121:	DVVDADENQD DDAEDSAVLV ADTQEEDGVA KGQVEADSEI CVAHTGSQEE LAEPDAVGSQ 
181:	TPIASAEETE VGEASDREGI AEAKATVCAD AVDACPDQVE AFEIEKVEDS ILDELQTELN 
241:	APADKTYEDV LAFDAVCSEA LSAFYAVPSD ETHFKVCGFY SPAIERTNCW LRSTLIVMQS 
301:	LPLEFKDLEM QKLWLSYKAG YDQCFVDKLV KSVPKSIILP QGGYVADFAY FFLSQCSFKA 
361:	YANWRCLECD MELKLQGLDA MFFYGDVVSH MCKCGNSMTL LSADIPYTLH FGVRDDKFCA 
421:	FYTPRKVFRA ACAVDVNDCH SMAVVEGKQI DGKVVTKFIG DKFDFMVGYG MTFSMSPFEL 
481:	AQLYGSCITP NVCFVKGDVI KVVRLVNAEV IVNPANGRMA HGAGVAGAIA EKAGSAFIKE 
541:	TSDMVKAQGV CQVGECYESA GGKLCKKVLN IVGPDARGHG KQCYSLLERA YQHINKCDNV 
601:	VTTLISAGIF SVPTDVSLTY LLGVVTKNVI LVSNNQDDFD VIEKCQVTSV AGTKALSLQL 
661:	AKNLCRDVKF VTNACSSLFS ESCFVSSYDV LQEVEALRHD IQLDDDARVF VQANMDCLPT 
721:	DWRLVNKFDS VDGVRTIKYF ECPGGIFVSS QGKKFGYVQN GSFKEASVSQ IRALLANKVD 
781:	VLCTVDGVNF RSCCVAEGEV FGKTLGSVFC DGINVTKVRC SAIYKGKVFF QYSDLSEADL 
841:	VAVKDAFGFD EPQLLKYYTM LGMCKWSVVV CGNYFAFKQS NNNCYINVAC LMLQHLSLKF 
901:	PKWQWQEAWN EFRSGKPLRF VSLVLAKGSF KFNEPSDSID FMRVVLREAD LSGATCNLEF 
961:	VCKCGVKQEQ RKGVDAVMHF GTLDKGDLVR GYNIACTCGS KLVHCTQFNV PFLICSNTPE 
1021:	GRKLPDDVVA ANIFTGGSVG HYTHVKCKPK YQLYDACNVN KVSEAKGNFT DCLYLKNLKQ 
1081:	TFSSVLTTFY LDDVKCVEYK PDLSQYYCES GKYYTKPIIK AQFRTFEKVD GVYTNFKLVG 
1141:	HSIAEKLNAK LGFDCNSPFV EYKITEWPTA TGDVVLASDD LYVSRYSSGC ITFGKPVVWL 
1201:	GHEEASLKSL TYFNRPSVVC ENKFNVLPVD VSEPTDKGPV PAAVLVTGVP GADASAGAGI 
1261:	AKEQKACASA SVEDQVVTEV RQEPSVSAAD VKEVKLNGVK KPVKVEGSVV VNDPTSETKV 
1321:	VKSLSIVDVY DMFLTGCKYV VWTANELSRL VNSPTVREYV KWGKGKIVTP AKLLLLRDEK 
1381:	QEFVAPKVVK AKAIACYCAV KWFLLYCFSW IKFNTDNKVI YTTEVASKLT FKLCCLAFKN 
1441:	ALQTFNWSVV SRGFFLVATV FLLWFNFLYA NVILSDFYLP NIGPLPTFVG QIVAWFKTTF 
1501:	GVSTICDFYQ VTDLGYRSSF CNGSMVCELC FSGFDMLDNY DAINVVQHVV DRRLSFDYIS 
1561:	LFKLVVELVI GYSLYTVCFY PLFVLIGMQL LTTWLPEFFM LETMHWSARL FVFVANMLPA 
1621:	FTLLRFYIVV TAMYKVYCLC RHVMYGCSKP GCLFCYKRNR SVRVKCSTVV GGSLRYYDVM 
1681:	ANGGTGFCTK HQWNCLNCNS WKPGNTFITH EAAADLSKEL KRPVNPTDSA YYSVTEVKQV 
1741:	GCSMRLFYER DGQRVYDDVN ASLFVDMNGL LHSKVKGVPE THVVVVENEA DKAGFLGAAV 
1801:	FYAQSLYRPM LMVEKKLITT ANTGLSVSRT MFDLYVDSLL NVLDVDRKSL TSFVNAAHNS 
1861:	LKEGVQLEQV MDTFIGCARR KCAIDSDVET KSITKSVMSA VNAGVDFTDE SCNNLVPTYV 
1921:	KSDTIVAADL GVLIQNNAKH VQANVAKAAN VACIWSVDAF NQLSADLQHR LRKACSKTGL 
1981:	KIKLTYNKQE ANVPILTTPF SLKGGAVFSR MLQWLFVANL ICFIVLWALM PTYAVHKSDM 
2041:	QLPLYASFKV IDNGVLRDVS VTDACFANKF NQFDQWYEST FGLAYYRNSK ACPVVVAVID 
2101:	QDIGHTLFNV PTTVLRYGFH VLHFITHAFA TDSVQCYTPH MQIPYDNFYA SGCVLSSLCT 
2161:	MLAHADGTPH PYCYTGGVMH NASLYSSLAP HVRYNLASSN GYIRFPEVVS EGIVRVVRTR 
2221:	SMTYCRVGLC EEAEEGICFN FNRSWVLNNP YYRAMPGTFC GRNAFDLIHQ VLGGLVRPID 
2281:	FFALTASSVA GAILAIIVVL AFYYLIKLK