Search TCDB: (?)
TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.11.1.1
Ammonia transporter and regulatory sensor, AmtB (Blauwkamp and Ninfa, 2003; Khademi et al., 2004).  It has a cleavable N-terminal signal peptide, and while Amt proteins in Gram-negative bacteria appear to utilize a signal peptide, the homologous proteins in Gram-positive organisms do not (Thornton et al. 2006).

Accession Number:P69681
Protein Name:Ammonia channel aka AmtB
Length:428
Molecular Weight:44515.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate ammonium, ammonia

Cross database links:

DIP: DIP-29874N
RefSeq: AP_001101.1    NP_414985.1   
Entrez Gene ID: 945084   
Pfam: PF00909   
BioCyc: EcoCyc:AMTB-MONOMER    ECOL168927:B0451-MONOMER   
KEGG: ecj:JW0441    eco:b0451   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0051739 F:ammonia transmembrane transporter activity
GO:0008519 F:ammonium transmembrane transporter activity
GO:0015292 F:uniporter activity
GO:0015696 P:ammonium transport
GO:0015670 P:carbon dioxide transport
GO:0055085 P:transmembrane transport

References (11)

[1] “An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli.”  van Heeswijk W.C.et.al.   8843440
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Cloning, sequencing, and characterization of Escherichia coli thioesterase II.”  Naggert J.et.al.   1645722
[5] “Intrinsic and extrinsic approaches for detecting genes in a bacterial genome.”  Borodovsky M.et.al.   7984428
[6] “Membrane topology of the Mep/Amt family of ammonium transporters.”  Thomas G.H.et.al.   10931328
[7] “The glnKamtB operon. A conserved gene pair in prokaryotes.”  Thomas G.H.et.al.   10637624
[8] “Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry.”  Blakey D.et.al.   12023896
[9] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[10] “The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli.”  Zheng L.et.al.   15563598
[11] “Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.”  Khademi S.et.al.   15361618
Structure:
1U77   1U7C   1U7G   1XQE   1XQF   2NMR   2NOP   2NOW   2NPC   2NPD   [...more]

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MKIATIKTGL ASLAMLPGLV MAAPAVADKA DNAFMMICTA LVLFMTIPGI ALFYGGLIRG 
61:	KNVLSMLTQV TVTFALVCIL WVVYGYSLAF GEGNNFFGNI NWLMLKNIEL TAVMGSIYQY 
121:	IHVAFQGSFA CITVGLIVGA LAERIRFSAV LIFVVVWLTL SYIPIAHMVW GGGLLASHGA 
181:	LDFAGGTVVH INAAIAGLVG AYLIGKRVGF GKEAFKPHNL PMVFTGTAIL YIGWFGFNAG 
241:	SAGTANEIAA LAFVNTVVAT AAAILGWIFG EWALRGKPSL LGACSGAIAG LVGVTPACGY 
301:	IGVGGALIIG VVAGLAGLWG VTMLKRLLRV DDPCDVFGVH GVCGIVGCIM TGIFAASSLG 
361:	GVGFAEGVTM GHQLLVQLES IAITIVWSGV VAFIGYKLAD LTVGLRVPEE QEREGLDVNS 
421:	HGENAYNA