TCDB » SEARCH

« See all members of the family

1.A.11.3.2 High-affinity ammonia transporter and sensor, Mep2 (also an NH₄⁺ sensor) (Javelle et al., 2003a; Rutherford et al., 2008) (has a pair of conserved his/his residues; mutation to his/glu as in Mep1 leads to uncoupling of transport and sensor functions (Boeckstaens et al., 2008))
Accession Number:	P41948
Protein Name:	Ammonium transporter MEP2
Length:	499
Molecular Weight:	53401.00
Species:	Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:	11
Location¹ / Topology² / Orientation³:	Membrane¹ / Multi-pass membrane protein²
Substrate	ammonia

Structure:
DIP:	DIP-4340N
RefSeq:	NP_014257.1
Entrez Gene ID:	855580
Pfam:	PF00909
KEGG:	sce:YNL142W
Gene Ontology GO:0016021 C:integral to membrane GO:0005886 C:plasma membrane GO:0008519 F:ammonium transmembrane transporter activity GO:0015696 P:ammonium transport GO:0019740 P:nitrogen utilization GO:0007124 P:pseudohyphal growth GO:0055085 P:transmembrane transport
References (7) [1] “A family of ammonium transporters in Saccharomyces cerevisiae.” Marini A.-M.et.al. 9234685 [2] “A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames.” Mallet L.et.al. 8619318 [3] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.” Philippsen P.et.al. 9169873 [4] “The MEP2 ammonium permease regulates pseudohyphal differentiation in Saccharomyces cerevisiae.” Lorenz M.C.et.al. 9482721 [5] “In vivo N-glycosylation of the mep2 high-affinity ammonium transporter of Saccharomyces cerevisiae reveals an extracytosolic N-terminus.” Marini A.-M.et.al. 11069679 [6] “Evidence that fungal MEP proteins mediate diffusion of the uncharged species NH(3) across the cytoplasmic membrane.” Soupene E.et.al. 11486013 [7] “A global topology map of the Saccharomyces cerevisiae membrane proteome.” Kim H.et.al. 16847258
5aex

UniProt (Universal Protein Resource)
CDD Search (Conserved Domain Database)

Predict TMSs (Predict number of transmembrane segments)

FASTA formatted sequence

1:	MSYNFTGTPT GEGTGGNSLT TDLNTQFDLA NMGWIGVASA GVWIMVPGIG LLYSGLSRKK 
61:	HALSLLWASM MASAVCIFQW FFWGYSLAFS HNTRGNGFIG TLEFFGFRNV LGAPSSVSSL 
121:	PDILFAVYQG MFAAVTGALM LGGACERARL FPMMVFLFLW MTIVYCPIAC WVWNAEGWLV 
181:	KLGSLDYAGG LCVHLTSGHG GLVYALILGK RNDPVTRKGM PKYKPHSVTS VVLGTVFLWF 
241:	GWMFFNGGSA GNATIRAWYS IMSTNLAAAC GGLTWMVIDY FRCGRKWTTV GLCSGIIAGL 
301:	VGITPAAGFV PIWSAVVIGV VTGAGCNLAV DLKSLLRIDD GLDCYSIHGV GGCIGSVLTG 
361:	IFAADYVNAT AGSYISPIDG GWINHHYKQV GYQLAGICAA LAWTVTVTSI LLLTMNAIPF 
421:	LKLRLSADEE ELGTDAAQIG EFTYEESTAY IPEPIRSKTS AQMPPPHENI DDKIVGNTDA 
481:	EKNSTPSDAS STKNTDHIV

1.A.11.3.2
High-affinity ammonia transporter and sensor, Mep2 (also an NH₄⁺ sensor) (Javelle et al., 2003a; Rutherford et al., 2008) (has a pair of conserved his/his residues; mutation to his/glu as in Mep1 leads to uncoupling of transport and sensor functions (Boeckstaens et al., 2008))

Cross database links:

Gene Ontology

References (7)

External Searches:

Analyze:

1.A.11.3.2High-affinity ammonia transporter and sensor, Mep2 (also an NH4+ sensor) (Javelle et al., 2003a; Rutherford et al., 2008) (has a pair of conserved his/his residues; mutation to his/glu as in Mep1 leads to uncoupling of transport and sensor functions (Boeckstaens et al., 2008))

Cross database links:

Gene Ontology

References (7)

External Searches:

Analyze:

1.A.11.3.2
High-affinity ammonia transporter and sensor, Mep2 (also an NH₄⁺ sensor) (Javelle et al., 2003a; Rutherford et al., 2008) (has a pair of conserved his/his residues; mutation to his/glu as in Mep1 leads to uncoupling of transport and sensor functions (Boeckstaens et al., 2008))