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1.A.12.1.2
Nuclear chloride channel-27, NCC27 or CLIC1 (Br- > Cl- > I-) (241 aas).  CLIC1 has two charged residues, K37 and R29, in its single TMS which are important for the biophysical properties of the channel (Averaimo et al. 2013).  A putative Lys37-Trp35 cation-pi interaction stabilizes the active dimeric form of the CLIC1 TMS in membranes (Peter et al. 2013).  This channel may play a role in cancer (Peretti et al. 2014).  A positively charged motif at the C-terminus of the single TMS enhances membrane partitioning and insertion via electrostatic contacts.  It also functions as an electrostatic plug to anchor the TMS in membranes and is involved in orientating the TMS with respect to the cis and trans faces of the membrane (Peter et al. 2014).  The CLIC1 protein accumulates in the circulating monocyte membrane during neurodegeneration (Carlini et al. 2020). The involvement of CLIC1 protein functions in physiological and in pathological conditions has been reviewed (Cianci and Verduci 2021).

Accession Number:O00299
Protein Name:CLIC1 aka NCC27
Length:241
Molecular Weight:26923.00
Species:Homo sapiens (Human) [9606]
Location1 / Topology2 / Orientation3: Nucleus1 / Single-pass membrane protein2
Substrate chloride

Cross database links:

RefSeq: NP_001279.2   
Entrez Gene ID: 1192   
OMIM: 602872  gene
KEGG: hsa:1192   

Gene Ontology

GO:0005903 C:brush border
GO:0034707 C:chloride channel complex
GO:0005737 C:cytoplasm
GO:0005624 C:membrane fraction
GO:0031965 C:nuclear membrane
GO:0005886 C:plasma membrane
GO:0005625 C:soluble fraction
GO:0005515 F:protein binding
GO:0005247 F:voltage-gated chloride channel activity
GO:0006821 P:chloride transport
GO:0007165 P:signal transduction

References (13)

[1] “Molecular cloning and expression of a chloride ion channel of cell nuclei.”  Valenzuela S.M.et.al.   9139710
[2] “A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons.”  Chuang J.Z.et.al.   10191309
[3] “Genes encoding three new members of the leukocyte antigen 6 superfamily and a novel member of Ig superfamily, together with genes encoding the regulatory nuclear chloride ion channel protein (hRNCC) and an N omega-N omega-dimethylarginine dimethylaminohydrolase homologue, are found in a 30-kb segment of the MHC class III region.”  Ribas G.et.al.   10384126
[4] “Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.”  Xie T.et.al.   14656967
[5] “The DNA sequence and analysis of human chromosome 6.”  Mungall A.J.et.al.   14574404
[6] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[7] “CLIC1 inserts from the aqueous phase into phospholipid membranes, where it functions as an anion channel.”  Tulk B.M.et.al.   11940526
[8] “Interaction of sedlin with chloride intracellular channel proteins.”  Fan L.et.al.   12681486
[9] “Formation of an unfolding intermediate state of soluble chloride intracellular channel protein CLIC1 at acidic pH.”  Fanucchi S.et.al.   18850721
[10] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[11] “Lysine acetylation targets protein complexes and co-regulates major cellular functions.”  Choudhary C.et.al.   19608861
[12] “Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution.”  Harrop S.J.et.al.   11551966
[13] “The intracellular chloride ion channel protein CLIC1 undergoes a redox-controlled structural transition.”  Littler D.R.et.al.   14613939
Structure:
1K0M   1K0N   1K0O   1RK4   3O3T   3P8W   3P90   3QR6   3SWL   3TGZ   [...more]

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MAEEQPQVEL FVKAGSDGAK IGNCPFSQRL FMVLWLKGVT FNVTTVDTKR RTETVQKLCP 
61:	GGQLPFLLYG TEVHTDTNKI EEFLEAVLCP PRYPKLAALN PESNTAGLDI FAKFSAYIKN 
121:	SNPALNDNLE KGLLKALKVL DNYLTSPLPE EVDETSAEDE GVSQRKFLDG NELTLADCNL 
181:	LPKLHIVQVV CKKYRGFTIP EAFRGVHRYL SNAYAREEFA STCPDDEEIE LAYEQVAKAL 
241:	K