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1.A.12.1.6
Chloride intracellular channel protein 4, CLIC4.  Regulates the histamine H3 receptor (Maeda et al., 2008)) Discriminates poorly between anions and cations (Singh and Ashley, 2007). 76% identical to CLIC5; it may play a role in cancer (Peretti et al. 2014). CLIC5 was the first mitochondrial chloride channel to be identified in the inner mitochondrial membrane, while CLIC4 is located predominantly in the outer mitochondrial membrane. Gururaja Rao et al. 2020 discussed the intracellular chloride channels, their roles in pathologies, such as cardiovascular, cancer, and neurodegenerative diseases, and developments concerning their usage as theraputic targets in humans.

Accession Number:Q9Y696
Protein Name:CLIC4
Length:253
Molecular Weight:28772.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cytoplasm1 / Single-pass membrane protein2
Substrate Cl-

Cross database links:

Genevestigator: Q9Y696
HEGENOM: HBG444630
RefSeq: NP_039234.1   
Entrez Gene ID: 25932   
OMIM: 606536  gene
KEGG: hsa:25932   

Gene Ontology

GO:0015629 C:actin cytoskeleton
GO:0034707 C:chloride channel complex
GO:0030659 C:cytoplasmic vesicle membrane
GO:0005902 C:microvillus
GO:0005634 C:nucleus
GO:0005886 C:plasma membrane
GO:0005625 C:soluble fraction
GO:0005247 F:voltage-gated chloride channel activity
GO:0030154 P:cell differentiation
GO:0006821 P:chloride transport
GO:0030336 P:negative regulation of cell migration

References (11)

[1] “A novel p64-related Cl- channel: subcellular distribution and nephron segment-specific expression.”  Edwards J.C.et.al.   10070163
[2] “A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons.”  Chuang J.Z.et.al.   10191309
[3] “Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.”  Wiemann S.et.al.   11230166
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers.”  Suh K.S.et.al.   17200346
[6] “CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation.”  Suh K.S.et.al.   17636002
[7] “CLIC4 interacts with histamine H3 receptor and enhances the receptor cell surface expression.”  Maeda K.et.al.   18302930
[8] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[9] “Lysine acetylation targets protein complexes and co-regulates major cellular functions.”  Choudhary C.et.al.   19608861
[10] “Crystal structure of the soluble form of the redox-regulated chloride ion channel protein CLIC4.”  Littler D.R.et.al.   16176272
[11] “Trimeric structure of the wild soluble chloride intracellular ion channel CLIC4 observed in crystals.”  Li Y.et.al.   16581025
Structure:
2AHE   2D2Z   3OQS     

External Searches:

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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV VFSVTTVDLK 
61:	RKPADLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC PPKYLKLSPK HPESNTAGMD 
121:	IFAKFSAYIK NSRPEANEAL ERGLLKTLQK LDEYLNSPLP DEIDENSMED IKFSTRKFLD 
181:	GNEMTLADCN LLPKLHIVKV VAKKYRNFDI PKEMTGIWRY LTNAYSRDEF TNTCPSDKEV 
241:	EIAYSDVAKR LTK