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1.A.14.2.3
The 7 TMS proton-sensitive Ca2+ leak channel, YetJ.  The activity and high resolution 3-d structure have been determined (Chang et al. 2014). BsYetJ in lipid nanodiscs is structurally different from those crystallized in detergents. Li et al. 2020 showed that the BsYetJ conformation is pH-sensitive in the apo state (lacking calcium), whereas in a calcium-containing solution it is stuck in an intermediate state, inert to pH changes. Only when the transmembrane calcium gradient is established can the calcium-release activity of holo-BsYetJ occur and be mediated by pH-dependent conformational changes, suggesting a dual gating mechanism. Conformational substates involved in the process and a key residue, D171, relevant to the gating of calcium were identified. Thus, BsYetJ/TMBIM6 is a pH-dependent, voltage-gated calcium channel (Li et al. 2020).

Accession Number:O31539
Protein Name:Uncharacterized protein YetJ
Length:214
Molecular Weight:23829.00
Species:Bacillus subtilis [1423]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Ca2+

Cross database links:

Entrez Gene ID: 938782   
Pfam: PF01027   
KEGG: bsu:BSU07200    bsu:BSU07200   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane

References (2)

[1] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[2] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
Structure:
4PGR   4PGS   4PGU   4PGV   4PGW   4TKQ   6NQ7   6NQ8   6NQ9      [...more]

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQATVHESKQ SIMQRILTVF VFTLLIATVG LFIGQFVPVA LMLPLSILEV AMIILAFWMR 
61:	RRKAVGYAFV YTFAFVSGIT LFPIVSHYAS IAGAYVVLEA FGSTFVIFAV LGTIGAKMKK 
121:	DLSFLWSFLL VAVLALAVVG IFNIFSPLNS AAMMAYSVIG TIVFSLYILY DLNQIKHRHI 
181:	TEDLIPVMAL SLYLDFINLF INLLRFFGIL SSDD