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1.A.20.1.1
BNip3 channel-forming protein (Bocharov et al., 2007). It is an apoptosis-inducing protein that can overcome BCL2 suppression and may play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2 (Ghavami et al. 2010). It is also involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, it participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (Nakamura et al. 2012). Platinum-based combination chemotherapy triggers cancer cell death through induction of BNIP3 and ROS, but not autophagy (Chung et al. 2020).  

Accession Number:Q12983
Protein Name:BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 aka BNip3
Length:194
Molecular Weight:21541.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Mitochondrion membrane1 / Single-pass membrane protein2
Substrate small molecules

Cross database links:

RefSeq: NP_004043.2   
Entrez Gene ID: 664   
Pfam: PF06553   
OMIM: 603293  gene
KEGG: hsa:664   

Gene Ontology

GO:0030425 C:dendrite
GO:0031307 C:integral to mitochondrial outer membrane
GO:0005635 C:nuclear envelope
GO:0005654 C:nucleoplasm
GO:0046982 F:protein heterodimerization activity
GO:0042803 F:protein homodimerization activity
GO:0006338 P:chromatin remodeling
GO:0051607 P:defense response to virus
GO:0006309 P:DNA fragmentation involved in apoptosis
GO:0006917 P:induction of apoptosis
GO:0044419 P:interspecies interaction between organisms
GO:0045837 P:negative regulation of membrane potential
GO:0008634 P:negative regulation of survival gene produc...
GO:0051402 P:neuron apoptosis
GO:0006800 P:oxygen and reactive oxygen species metaboli...
GO:0046902 P:regulation of mitochondrial membrane permea...
GO:0001666 P:response to hypoxia

References (6)

[1] “Adenovirus E1B 19 kDa and Bcl-2 proteins interact with a common set of cellular proteins.”  Boyd J.M.et.al.   7954800
[2] “The E1B 19K/Bcl-2-binding protein Nip3 is a dimeric mitochondrial protein that activates apoptosis.”  Chen G.et.al.   9396766
[3] “The DNA sequence and comparative analysis of human chromosome 10.”  Deloukas P.et.al.   15164054
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “A novel adenovirus E1B19K-binding protein B5 inhibits apoptosis induced by Nip3 by forming a heterodimer through the C-terminal hydrophobic region.”  Ohi N.et.al.   10381623
[6] “Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis.”  Wang B.et.al.   19007248
Structure:
2J5D   2KA1   2KA2     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSQNGAPGMQ EESLQGSWVE LHFSNNGNGG SVPASVSIYN GDMEKILLDA QHESGRSSSK 
61:	SSHCDSPPRS QTPQDTNRAS ETDTHSIGEK NSSQSEEDDI ERRKEVESIL KKNSDWIWDW 
121:	SSRPENIPPK EFLFKHPKRT ATLSMRNTSV MKKGGIFSAE FLKVFLPSLL LSHLLAIGLG 
181:	IYIGRRLTTS TSTF