TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.22.1.5
The pentameric MscL channel (Iscla et al., 2011).  The high resolution structure of a proposed closed but expanded tetrameric intermediate state has been determined (Liu et al. 2009). Adhesive forces to surfaces play an important role, next to other established driving forces, in staphylococcal MscL channel gating (Carniello et al. 2020). Thus, transmembrane antibiotic uptake and solute efflux in infectious staphylococcal biofilms is greatly stimulated when bacteria experience adhesion forces from surfaces as in biofilms.

Accession Number:P68805
Protein Name:Large-conductance mechanosensitive channel
Length:120
Molecular Weight:13616.00
Species:Staphylococcus aureus (strain NCTC 8325) [93061]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate ions

Cross database links:

HEGENOM: HBG727604
Pfam: PF01741   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005216 F:ion channel activity

References (1)

[1] “Functional and structural conservation in the mechanosensitive channel mscL implicates elements crucial for mechanosensation.”  Moe P.C.et.al.   9632260

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MLKEFKEFAL KGNVLDLAIA VVMGAAFNKI ISSLVENIIM PLIGKIFGSV DFAKEWSFWG 
61:	IKYGLFIQSV IDFIIIAFAL FIFVKIANTL MKKEEAEEEA VVEENVVLLT EIRDLLREKK