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1.A.26.1.2
The Mg2+ transporter, MgtE. The crystal structure of the N-terminal hydrophilic domain has been determined to 2.3 Å resolution (Hattori et al., 2007) (>50% identical to 9.A.19.1.1), while the C-terminal transmembrane domain has been determined at 2.2 Å resolution (Takeda et al. 2014). The structure reveals a homodimer with the channel at the interface of the two subunits.  There is a plug helix connecting the two domains, and the cytoplasmic domain possesses multiple Mg2+ binding sites at the cytoplasmic face that can bind Mg2+, Mn2+ and Ca2+. Dissociation of Mg2+ ions from the cytoplasmic domain induces structural changes in the cytoplasmic domain, leading to channel opening (Wang et al. 2023). Novel crystal structures of the Mg2+-bound MgtE cytoplasmic domains from two different bacterial species, Chryseobacterium hispalense and Clostridiales bacterium allowed identification of multiple Mg2+ binding sites, including ones that were not observed in the previous MgtE structure. These structures reveal the conservation and diversity of the cytoplasmic Mg2+ binding site in MgtE family proteins (Wang et al. 2023).

Accession Number:Q5SMG8
Protein Name:Mg2+ transporter MgtE
Length:450
Molecular Weight:50078.00
Species:Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [300852]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate calcium(2+), magnesium(2+), manganese(2+)

Cross database links:

RefSeq: YP_144326.1   
Entrez Gene ID: 3168925   
Pfam: PF00571    PF01769    PF03448   
BioCyc: TTHE300852:TTHA1060-MONOMER   
KEGG: ttj:TTHA1060    ttj:TTHA1060   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0003824 F:catalytic activity
GO:0008324 F:cation transmembrane transporter activity
GO:0000287 F:magnesium ion binding
GO:0042803 F:protein homodimerization activity
GO:0015693 P:magnesium ion transport
GO:0008152 P:metabolic process
GO:0015095 F:magnesium ion transmembrane transporter activity

References (2)

[1] “Crystal structure of the MgtE Mg(2+) transporter.”  Hattori M.et.al.   17700703
[2] “Crystal structure of the MgtE Mg(2+) transporter.”  Hattori M.et.al.   17700703
Structure:
2YVX   2YVY   2YVZ   2ZY9   4U9L   4U9N   4WIB   5X9G   5X9H      [...more]

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEEKLAVSLQ EALQEGDTRA LREVLEEIHP QDLLALWDEL KGEHRYVVLT LLPKAKAAEV 
61:	LSHLSPEEQA EYLKTLPPWR LREILEELSL DDLADALQAV RKEDPAYFQR LKDLLDPRTR 
121:	AEVEALARYE EDEAGGLMTP EYVAVREGMT VEEVLRFLRR AAPDAETIYY IYVVDEKGRL 
181:	KGVLSLRDLI VADPRTRVAE IMNPKVVYVR TDTDQEEVAR LMADYDFTVL PVVDEEGRLV 
241:	GIVTVDDVLD VLEAEATEDI HKLGAVDVPD LVYSEAGPVA LWLARVRWLV ILILTGMVTS 
301:	SILQGFESVL EAVTALAFYV PVLLGTGGNT GNQSATLIIR ALATRDLDLR DWRRVFLKEM 
361:	GVGLLLGLTL SFLLVGKVYW DGHPLLLPVV GVSLVLIVFF ANLVGAMLPF LLRRLGVDPA 
421:	LVSNPLVATL SDVTGLLIYL SVARLLLEAV