TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.38.1.3
The GPCR-type G protein, COLD1, of 455 aas and 9 TMSs in a 5 + 4 TMS arrangement. In cold tolerant cultivars (AC Q7X7S8) Met-187 is replaced by Lys-187. This polymorphism is associated with divergence in chilling tolerance of rice cultivars. COLD1 confers adaptation of japonica rice to chilling and originated from the Chinese wild populations of Oryza rufipogon (Ma et al. 2015).
Overexpression of COLD1(jap) significantly enhances chilling tolerance, whereas rice lines with a deficiency or downregulation of COLD1(jap) are sensitive to cold. COLD1 encodes a regulator of G-protein signaling that localizes to the plasma membrane and endoplasmic reticulum (ER). It interacts with the G-protein α subunit to activate the Ca2+ channel for sensing low temperature and to accelerate G-protein GTPase activity (Ma et al. 2015).

Accession Number:A2XX57
Protein Name:GPCR-type G protein COLD1
Length:468
Molecular Weight:53407.00
Species:Oryza sativa subsp. indica (Rice) [39946]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MGWGAVVYEG GVVGASLVGL GWAGLWFLNR RLYKEYEERR ALVQILFGLV FAFSCNLFQL 
61:	VLFEILPVLS KHARFLNWHL DLFCLILLLV FVLPYYHCYL LLRNSGVRRE RALLVAALFL 
121:	LVFLYGFWRM GIHFPMPSPE KGFFTMPQLV SRIGVIGVSV MAVLSGFGAV NLPYSYLSLF 
181:	IREIDEMDIK TLERQLMQSM ETCIAKKKKI VLSKMEMERI QGSEEKLKAR SFLKRIVGTV 
241:	VRSVQEDQTE QDIKSLDAEV QALEELSKQL FLEIYELRQA KIAAAFSRTW RGHAQNLLGY 
301:	ALSVYCVYKM LKSLQSVVFK EAGSVDPVTM TITIFLRHFD IGIDVTLLSQ YISLIFIGML 
361:	VVISVRGFLA NVMKFFFAVS RVGSGSTTNV VLFLSEIMGM YFISSILLIR KSLANEYRVI 
421:	ITDVLGGDIQ FDFYHRWFDA IFVASAFLSL LLISAQYTSR QTDKHPID