1.A.43.1.1 The camphor resistance protein, CrcB or FluC (Hu et al. 1996; Sand et al. 2003). Exports fluoride selectively over chloride by an anion open channel mechanism (Stockbridge et al. 2013). The active transporter is a dimer of 4 TMS subunits arranged in an antiparallel transmembrane orientation (Stockbridge et al. 2014). In bacteria lacking Fluc, F- accumulates when the external medium is
acidified as a predicted function of the transmembrane pH gradient.
This weak acid accumulation effect, which results from the high pKa
(3.4) and membrane permeability of HF, is abolished by Fluc channels (Ji et al. 2014). A proper tubulin network is required for functional Cx43 GJ channels, and mefloquineis a gap junction inhibitor (Sand et al. 2003). Exports fluoride selectively over chloride by an anion open channel mechanism (Stockbridge et al. 2013). The active transporter is a dimer of 4 TMS subunits arranged in an antiparallel transmembrane orientation (Stockbridge et al. 2014). In bacteria lacking Fluc, F- accumulates when the external medium is
acidified as a predicted function of the transmembrane pH gradient.
This weak acid accumulation effect, which results from the high pKa
(3.4) and membrane permeability of HF, is abolished by Fluc channels (Ji et al. 2014). A proper tubulin network is required for functional Cx43 GJ channels, and mefloquineis a gap junction inhibitor (Picoli et al. 2019).
.
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Accession Number: | P37002 |
Protein Name: | CrcB aka b0624 aka JW0619 |
Length: | 127 |
Molecular Weight: | 13777.00 |
Species: | Escherichia coli [83333] |
Number of TMSs: | 4 |
Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Multi-pass membrane protein2 |
Substrate |
fluoride, chloride |
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RefSeq: |
AP_001273.1
NP_415157.1
|
Entrez Gene ID: |
945798
|
Pfam: |
PF02537
|
BioCyc: |
EcoCyc:EG12209-MONOMER
ECOL168927:B0624-MONOMER
|
KEGG: |
ecj:JW0619
eco:b0624
|
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[1] “Overproduction of three genes leads to camphor resistance and chromosome condensation in Escherichia coli.” Hu K.H. et.al. 8844142
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.” Oshima T. et.al. 8905232
[3] “The complete genome sequence of Escherichia coli K-12.” Blattner F.R. et.al. 9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.” Hayashi K. et.al. 16738553
[5] “Phenotypic characterization of overexpression or deletion of the Escherichia coli crcA, cspE and crcB genes.” Sand O. et.al. 12904550
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.” Daley D.O. et.al. 15919996
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1: MLQLLLAVFI GGGTGSVARW LLSMRFNPLH QAIPLGTLTA NLIGAFIIGI GFAWFSRMTN
61: IDPVWKVLIT TGFCGGLTTF STFSAEVVFL LQEGRFGWAL LNVFVNLLGS FAMTALAFWL
121: FSASTAH