1.A.61.1.2 Flock House virus (FHV) capsid protein-α of 407 aas; Its C-terminal 44 aas comprise a lytic peptide, one of the γ-peptides that inserts into endomembranes forming pores. Capsid
protein alpha self-assembles to form an icosahedral procapsid with a
T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid
proteins trimers. 240 calcium ions are incorporated per
capsid during assembly. The capsid encapsulates the two genomic RNAs.
Capsid maturation occurs via autoproteolytic cleavage of capsid protein
alpha, generating capsid protein beta and the membrane-active peptide
gamma. Peptide
γ is a membrane-permeabilizing peptide produced during virus maturation,
thereby creating the infectious virion. After endocytosis into the host
cell, peptide gamma is exposed in endosomes, where it
permeabilizes the endosomal membrane, facilitating translocation of
viral capsid or RNA into the cytoplasm. Nangia et al. 2019 shed light on the actions of varied forms of the FHV lytic peptide including membrane insertion, trans-membrane stability, peptide oligomerization, water permeation activity and dynamic pore formation.
|
Accession Number: | P12870 |
Protein Name: | Capsid protein alpha |
Length: | 407 |
Molecular Weight: | 43710.00 |
Species: | Flock house virus (FHV) [12287] |
Number of TMSs: | 1 |
Location1 / Topology2 / Orientation3: |
Virion1 |
Substrate |
protein, nucleic acid |
---|
1: MVNNNRPRRQ RAQRVVVTTT QTAPVPQQNV PRNGRRRRNR TRRNRRRVRG MNMAALTRLS
61: QPGLAFLKCA FAPPDFNTDP GKGIPDRFEG KVVSRKDVLN QSISFTAGQD TFILIAPTPG
121: VAYWSASVPA GTFPTSATTF NPVNYPGFTS MFGTTSTSRS DQVSSFRYAS MNVGIYPTSN
181: LMQFAGSITV WKCPVKLSTV QFPVATDPAT SSLVHTLVGL DGVLAVGPDN FSESFIKGVF
241: SQSACNEPDF EFNDILEGIQ TLPPANVSLG STGQPFTMDS GAEATSGVVG WGNMDTIVIR
301: VSAPEGAVNS AILKAWSCIE YRPNPNAMLY QFGHDSPPLD EVALQEYRTV ARSLPVAVIA
361: AQNASMWERV KSIIKSSLAA ASNIPGPIGV AASGISGLSA LFEGFGF