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1.A.79.1.3
Lysosomal systemic RNA interference defective protein-2, SidT2 of 832 aas and 12 TMSs. It increases the uptake of exogenous dsRNA and DNA (Aizawa et al. 2016).  RNA and DNA are directly taken up by lysosomes in an ATP-dependent manner and degraded. SIDT2 has been reported to mediate RNA translocation during RNA autophagy and DNA translocation during DNA autophagy. Knockdown of Sidt2 inhibited, up to ~50%, total RNA degradation at the cellular level, independently of macroautophagy (Aizawa et al. 2016).  RNA autophagy plays a role in constitutive cellular RNA degradation. SIDT2 also takes up single stranded oligonucleotides into cells (Takahashi et al. 2017). Contu et al. 2017 showed that three cytosolic YXXPhi motifs in SIDT2 are required for the lysosomal localization of SIDT2, and that SIDT2 interacts with adaptor protein complexes AP-1 and AP-2.  On the other hand, Méndez-Acevedo et al. 2017 reported that this protein and SIDT1 transport cholesterol and not RNA. SIDT2 and RNautophagy promote tumor development (Nguyen et al. 2019). The cytosolic domain of SIDT2 carries an arginine-rich motif that binds to RNA/DNA and is important for the direct transport of nucleic acids into lysosomes (Hase et al. 2020). Sidt2 influences the three inflammatory signal pathways, eventually leading to the damage of glomerular mesangial cells in mice (Sun et al. 2020). The variant rs1784042 of the SIDT2 gene is associated with the metabolic syndrome through Low HDL-c levels (León-Reyes et al. 2020). Sidt2 enhances glucose uptake in peripheral tissues upon insulin stimulation (Xiong et al. 2020).

Accession Number:Q8NBJ9
Protein Name:SID1 transmembrane family member 2
Length:832
Molecular Weight:94454.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Lysosome membrane1 / Multi-pass membrane protein2
Substrate cholesterol, ribonucleic acid, double-stranded RNA, double-stranded DNA, single-stranded DNA

Cross database links:

Entrez Gene ID: 51092   
KEGG: hsa:51092    hsa:51092   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005765 C:lysosomal membrane

References (16)

[1] “Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics.”  Lai C.-H.et.al.   10810093
[2] “The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.”  Clark H.F.et.al.   12975309
[3] “Signal peptide prediction based on analysis of experimentally verified cleavage sites.”  Zhang Z.et.al.   15340161
[4] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[5] “Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.”  Otsuki T.et.al.   16303743
[6] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.”  Olsen J.V.et.al.   17081983
[7] “Integral and associated lysosomal membrane proteins.”  Schroeder B.et.al.   17897319
[8] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[9] “Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics.”  Lai C.-H.et.al.   10810093
[10] “The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.”  Clark H.F.et.al.   12975309
[11] “Signal peptide prediction based on analysis of experimentally verified cleavage sites.”  Zhang Z.et.al.   15340161
[12] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[13] “Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.”  Otsuki T.et.al.   16303743
[14] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.”  Olsen J.V.et.al.   17081983
[15] “Integral and associated lysosomal membrane proteins.”  Schroeder B.et.al.   17897319
[16] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MFALGLPFLV LLVASVESHL GVLGPKNVSQ KDAEFERTYV DEVNSELVNI YTFNHTVTRN 
61:	RTEGVRVSVN VLNKQKGAPL LFVVRQKEAV VSFQVPLILR GMFQRKYLYQ KVERTLCQPP 
121:	TKNESEIQFF YVDVSTLSPV NTTYQLRVSR MDDFVLRTGE QFSFNTTAAQ PQYFKYEFPE 
181:	GVDSVIVKVT SNKAFPCSVI SIQDVLCPVY DLDNNVAFIG MYQTMTKKAA ITVQRKDFPS 
241:	NSFYVVVVVK TEDQACGGSL PFYPFAEDEP VDQGHRQKTL SVLVSQAVTS EAYVSGMLFC 
301:	LGIFLSFYLL TVLLACWENW RQKKKTLLVA IDRACPESGH PRVLADSFPG SSPYEGYNYG 
361:	SFENVSGSTD GLVDSAGTGD LSYGYQGRSF EPVGTRPRVD SMSSVEEDDY DTLTDIDSDK 
421:	NVIRTKQYLY VADLARKDKR VLRKKYQIYF WNIATIAVFY ALPVVQLVIT YQTVVNVTGN 
481:	QDICYYNFLC AHPLGNLSAF NNILSNLGYI LLGLLFLLII LQREINHNRA LLRNDLCALE 
541:	CGIPKHFGLF YAMGTALMME GLLSACYHVC PNYTNFQFDT SFMYMIAGLC MLKLYQKRHP 
601:	DINASAYSAY ACLAIVIFFS VLGVVFGKGN TAFWIVFSII HIIATLLLST QLYYMGRWKL 
661:	DSGIFRRILH VLYTDCIRQC SGPLYVDRMV LLVMGNVINW SLAAYGLIMR PNDFASYLLA 
721:	IGICNLLLYF AFYIIMKLRS GERIKLIPLL CIVCTSVVWG FALFFFFQGL STWQKTPAES 
781:	REHNRDCILL DFFDDHDIWH FLSSIAMFGS FLVLLTLDDD LDTVQRDKIY VF