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1.A.8.1.1
Glycerol facilitator, GlpF. Transports various polyols with decreasing rates as size increases (Heller et al. 1980); also transports arsenite (As(III) and antimonite (Sb(III)) (Meng et al., 2004). Oligomerization may play a role in determining the rates of transport (Klein et al. 2019). AQP water permeability through GlpF can be regulated by lipid bilayer asymmetry and the transmembrane potential. The conserved Arg in the selectivity filter and positions and dynamics of multiple other pore lining residues modulate water passage through GlpF (Pluhackova et al. 2022).

Accession Number:P0AER0
Protein Name:Glycerol uptake facilitator protein GLPF aka B3927
Length:281
Molecular Weight:29780.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate arsenite(3-), polyol, glycerol

Cross database links:

DIP: DIP-47976N
RefSeq: AP_003882.1    NP_418362.1   
Entrez Gene ID: 948422   
Pfam: PF00230   
BioCyc: EcoCyc:GLPF-MONOMER    ECOL168927:B3927-MONOMER   
KEGG: ecj:JW3898    eco:b3927   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015254 F:glycerol channel activity
GO:0046872 F:metal ion binding
GO:0071288 P:cellular response to mercury ion
GO:0015793 P:glycerol transport
GO:0055085 P:transmembrane transport

References (11)

[1] “Nucleotide sequence of the region encompassing the glpKF operon and its upstream region containing a bent DNA sequence of Escherichia coli.”  Muramatsu S.et.al.   2544860
[2] “Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12.”  Weissenborn D.L.et.al.   1372899
[3] “Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes.”  Plunkett G. IIIet.al.   8346018
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli.”  Heller K.B.et.al.   6998951
[7] “Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli.”  Borgnia M.J.et.al.   11226336
[8] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[9] “Structure of a glycerol-conducting channel and the basis for its selectivity.”  Fu D.et.al.   11039922
[10] “Fraternal twins: AQP1 and GlpF.”  Unger V.M.et.al.   11101882
[11] “Control of the selectivity of the aquaporin water channel family by global orientational tuning.”  Tajkhorshid E.et.al.   11964478
Structure:
1FX8   1LDA   1LDF   1LDI     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MSQTSTLKGQ CIAEFLGTGL LIFFGVGCVA ALKVAGASFG QWEISVIWGL GVAMAIYLTA 
61:	GVSGAHLNPA VTIALWLFAC FDKRKVIPFI VSQVAGAFCA AALVYGLYYN LFFDFEQTHH 
121:	IVRGSVESVD LAGTFSTYPN PHINFVQAFA VEMVITAILM GLILALTDDG NGVPRGPLAP 
181:	LLIGLLIAVI GASMGPLTGF AMNPARDFGP KVFAWLAGWG NVAFTGGRDI PYFLVPLFGP 
241:	IVGAIVGAFA YRKLIGRHLP CDICVVEEKE TTTPSEQKAS L