1.B.55.3.1
Cellulose synthase operon, protein C of 1157 aas and up to 18 C-terminal (720 - 1157 aas) β-strands with a single N-terminal α-TMS, BcsC or YhjL (Zogaj et al. 2001). Translocation across the outer membrane occurs through the BcsC porin,
which extends into the periplasm via 19 tetra-tricopeptide repeats
(TPR). Acheson et al. 2019 presented the crystal structure of a truncated BcsC,
encompassing the last TPR repeat and the complete outer membrane channel domain, revealing a 16-stranded, β barrel pore architecture.
The pore is blocked by an extracellular gating loop, while the extended C
terminus inserts deeply into the channel and positions a conserved Trp
residue near its extracellular exit. The channel is lined with
hydrophilic and aromatic residues suggesting a mechanism for facilitated
cellulose diffusion based on aromatic stacking and hydrogen bonding (Acheson et al. 2019).
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| Accession Number: | P37650 |
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| Protein Name: | Cellulose synthase operon protein C |
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| Length: | 1157 |
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| Molecular Weight: | 127724.00 |
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| Species: | Escherichia coli (strain K12) [83333] |
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| Substrate |
D-glucosyl group |
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1: MRKFTLNIFT LSLGLAVMPM VEAAPTAQQQ LLEQVRLGEA THREDLVQQS LYRLELIDPN
61: NPDVVAARFR SLLRQGDIDG AQKQLDRLSQ LAPSSNAYKS SRTTMLLSTP DGRQALQQAR
121: LQATTGHAEE AVASYNKLFN GAPPEGDIAV EYWSTVAKIP ARRGEAINQL KRINADAPGN
181: TGLQNNLALL LFSSDRRDEG FAVLEQMAKS NAGREGASKI WYGQIKDMPV SDASVSALKK
241: YLSIFSDGDS VAAAQSQLAE QQKQLADPAF RARAQGLAAV DSGMAGKAIP ELQQAVRANP
301: KDSEALGALG QAYSQKGDRA NAVANLEKAL ALDPHSSNND KWNSLLKVNR YWLAIQQGDA
361: ALKANNPDRA ERLFQQARNV DNTDSYAVLG LGDVAMARKD YPAAERYYQQ TLRMDSGNTN
421: AVRGLANIYR QQSPEKAEAF IASLSASQRR SIDDIERSLQ NDRLAQQAEA LENQGKWAQA
481: AALQRQRLAL DPGSVWITYR LSQDLWQAGQ RSQADTLMRN LAQQKSNDPE QVYAYGLYLS
541: GHDQDRAALA HINSLPRAQW NSNIQELVNR LQSDQVLETA NRLRESGKEA EAEAMLRQQP
601: PSTRIDLTLA DWAQQRRDYT AARAAYQNVL TREPANADAI LGLTEVDIAA GDKAAARSQL
661: AKLPATDNAS LNTQRRVALA QAQLGDTAAA QRTFNKLIPQ AKSQPPSMES AMVLRDGAKF
721: EAQAGDPTQA LETYKDAMVA SGVTTTRPQD NDTFTRLTRN DEKDDWLKRG VRSDAADLYR
781: QQDLNVTLEH DYWGSSGTGG YSDLKAHTTM LQVDAPYSDG RMFFRSDFVN MNVGSFSTNA
841: DGKWDDNWGT CTLQDCSGNR SQSDSGASVA VGWRNDVWSW DIGTTPMGFN VVDVVGGISY
901: SDDIGPLGYT VNAHRRPISS SLLAFGGQKD SPSNTGKKWG GVRADGVGLS LSYDKGEANG
961: VWASLSGDQL TGKNVEDNWR VRWMTGYYYK VINQNNRRVT IGLNNMIWHY DKDLSGYSLG
1021: QGGYYSPQEY LSFAIPVMWR ERTENWSWEL GASGSWSHSR TKTMPRYPLM NLIPTDWQEE
1081: AARQSNDGGS SQGFGYTARA LLERRVTSNW FVGTAIDIQQ AKDYAPSHFL LYVRYSAAGW
1141: QGDMDLPPQP LIPYADW