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1.C.21.1.5 Nukacin ISK-1 of 57 aas (Okuda et al., 2008). It is active on Gram-positive bacteria, including Lactobacillus sakei, Leuconostoc mesenteroides and Pediococcus pentosaceus. The bactericidal activity is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores (Aso et al. 2004). It is processed and secreted by NukT (TC# 3.A.1.111.7) (Zheng et al. 2017).
Accession Number:	Q9KWM4
Protein Name:	ISK-1 aka NukA
Length:	57
Molecular Weight:	6403.00
Species:	Staphylococcus warneri [1292]
Location¹ / Topology² / Orientation³:	Secreted¹
Substrate	molecule, water, polyelectrolyte macromolecule

Structure:
RefSeq:	NP_940772.1
Entrez Gene ID:	2648465
Pfam:	PF04604
Gene Ontology GO:0005576 C:extracellular region GO:0005102 F:receptor binding GO:0019835 P:cytolysis GO:0042742 P:defense response to bacterium GO:0019748 P:secondary metabolic process
References (5) [1] “A novel lantibiotic, nukacin ISK-1, of Staphylococcus warneri ISK-1: cloning of the structural gene and identification of the structure.” Sashihara T.et.al. 11193411 [2] “Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1.” Aso Y.et.al. 15322349 [3] “Description of complete DNA sequence of two plasmids from the nukacin ISK-1 producer, Staphylococcus warneri ISK-1.” Aso Y.et.al. 15737403 [4] “Heterologous expression and functional analysis of the gene cluster for the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1.” Aso Y.et.al. 16233732 [5] “Lysine-oriented charges trigger the membrane binding and activity of nukacin ISK-1.” Asaduzzaman S.M.et.al. 16957223
5Z5Q 5Z5R

Predict TMSs (Predict number of transmembrane segments)

1:	MENSKVMKDI EVANLLEEVQ EDELNEVLGA KKKSGVIPTV SHDCHMNSFQ FVFTCCS