1.C.57.3.5
Multifunctional-autoprocessing repeats-in-toxin, RtxA or Rtx, of 4558 aas. It is the precursor
of a multifunctional toxin that causes destruction of the actin
cytoskeleton by covalent cross-linking of actin and inactivation of Rho
GTPases when translocated into the host cytoplasm (Satchell 2015). Upon translocation into the host cell, it undergoes autoprocessing in cis
mediated by the peptidase C80 domain (also named CPD domain). The
protease activity is activated upon binding inositol hexakisphosphate
(InsP6), present at the host cell membrane, delivering the cysteine
protease domain-containing toxin F3 chain to the host cytosol (Sheahan et al. 2007, Shen et al. 2009). It forms a pore in the plasma membrane of a eukaryotic cell to deliver the toxin (Woida and Satchell 2018).
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| Accession Number: | Q9KS12 |
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| Protein Name: | Multifunctional-autoprocessing repeats-in-toxin |
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| Length: | 4558 |
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| Molecular Weight: | 485355.00 |
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| Species: | Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [243277] |
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| Location1 / Topology2 / Orientation3: |
Host cytoplasm1 |
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| Substrate |
ion, protein polypeptide chain |
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1: MVFYLIPKRR VWLMGKPFWR SVEYFFTGNY SADDGNNNIV AIGFGGQIHA YGGDDHVTVG
61: SIGATVYTGS GNDTVVGGSA YLKVEDSTGH LIVKGAAGYA DINKSGDGNV SFAGAAGGVS
121: IDHLGNHGDV SYGGAAAYNG ITRKGLSGNV TFAGAGGYNA LWHETNQGNL SFTGAGAGNK
181: LDRTWSNRYQ GSHGDVTFDG AGAANSISSR VETGNITFRG AGADNHLVRK GKVGDITLQG
241: AGASNRIERT HQAEDVYTQT RGNIRFEGVG GYNSLYSDVA HGDIHFSGGG AYNTIIRKGS
301: GNDFAKEGMT NAKADEIVLT KAVMSGSWIG QDHHVTAVKS ASEPNTYLFA FADSTYTKIN
361: KVQLRNDPQT GELKYYSTAW YKEVNHLSNL ANQDISDNGG FTAVNINGAY TLSDLKVEHQ
421: QSVTVHAVEK SLTEYEWVTY ANGAVIDAKE VSLSDAKMGG HAIYADGTKV DVKAVKSNRQ
481: PNTYIYAKVL GPYTKIVVVE LANDPETGAL KYQARSWYKE GDHTANIANQ DISSATGYNP
541: MGKGGYSLSD LHYSVNAVRS TSETVADIEE YTDQTLFKPA NDSGESSGDV RFNGAGGGNV
601: IKSNVTRGNV HFNGGGIANV ILHSSQFGNT EFNGGGAANV IVKSGEEGDL TFRGAGLANV
661: LVHQSEQGKM DVYAGGAVNV LVRLGDGQYL AHLLAYGNIS VQKGSGDSRV VMLGGYNTHT
721: QIGSGNGLWL AAGGFNVMTQ VGKGDVAAVL AGGANVLTKM GEGELTSGML GGANVITHIS
781: NDDQLSNTTA VALGGANILT KKGKGNTLAV MGGGANVLTH VGDGTTTGVM VGGANILTKV
841: GNGDTTGILL GVGNVLTHVG DGQTLGVMGA AGNIFTKVGD GTSIAVMIGA GNIFTHVGEG
901: NAWALMGGLG NVFTKVGNGD ALALMVAEAN VFTHIGDGMS VALMLAKGNV ATKVGNGTTL
961: AAMVGNVNIF THIGHGSTFA AMIGQANIMT KVGNDLTAAL MVGKANIMTH VGDGTSLGLF
1021: AGEVNVMTKV GNGTTLAAMF GKANIMTHVG DGLTGVLALG EANIVTKLGD DFMGVVAAAK
1081: ANVVTHVGDA TTAAVLAGKG NILTKVGEGT TVGLLISDVG NVMTHVGDGT TIGIAKGKAN
1141: LITKVGDGLG VNVTWGQANV FTQVGDGDRY NFAKGEANLI TKVGDGQEVS VVQGEANIIT
1201: HVGNGDDYTG AWGKANVITK VGHGQNVVLA KGEANIVTQV GDGDSFNALW SKGNIVTKVG
1261: DGMQVTAAKG QANITTTVGN GLNVTAAYGD ANINTKVGDG VSVNVAWGKY NINTKVGDGL
1321: NVAVMKGKAN ANIHVGDGLN INASYAQNNV AIKVGNGDFY SLAVASSNTS SNKLSALFDN
1381: IKQTVLGVGG SQAINYLVQG DEASSSGTHK GRGAIATPEI TKLDGFQMDA IKEVSSDLGD
1441: SLTGSVTKVD TPDLNKMQHA LNVDDSSVQA PNLIVNGDFE LGEHGWQSTH GVEASYAGSV
1501: YGVEGEGHGA RVTELDTYTN TSLYQDLANL AQGEVIAVSF DFAKRAGLSN NEGIEVLWNG
1561: EVVFSSSGDE SAWQQKNLKL TAQAGSNRIE FKGTGHNDGL GYILDNVVAT SESSQQANAI
1621: REHATQNPAA QNALSDKERA EADRQRLEQE KQKQLDAVAG SQSQLESTDQ QALENNGQAQ
1681: RDAVKEESEA VTAELAKLAQ GLDVLDGQAT HTGESGDQWR NDFAGGLLDG VQSQLDDAKQ
1741: LANDKIAAAK QTLSDNNSKV KESVAKSEAG VAQGEQNRAG VEQDIADAQA DAEKRKADAL
1801: AKGKDAQQAE SDAHHAVNNA QSRGDRDVQL AENKANQAQA DAQGAKQNEG DRPDRQGVTG
1861: SGLSGNAHSV EGAGETDSHV NTDSQTNADG RFSEGLTEQE QEALEGATNA VNRLQINAGI
1921: RAKNSVSSMT SMFSETNSKS IVVPTKVSPE PERQEVTRRD VRISGVNLES LSAVQGSQPT
1981: GQLASKSVPG FKSHFASTSI GIENELSGLV VVLPKNSAQT FGYVHDSQGN PLFMLTKDMN
2041: QGGYSNPVGI NDIQGVNNWQ THTIELVTYP SEISDTAAVE SRKEAMLWLA KEFTDHINQS
2101: NHQSLPHLVS DDGRFTLVIS NSKHLIAAGN GTSIDAQGKT IGMTPSGQQA TMAISAKEFG
2161: TSSSPEVRLL ESAPWYQAGL RDEFLANAKN TTLDDPATAQ NVYAYLTSVY SKTADLAKEY
2221: GIYINDWDPA SEGFSPNAQG LTDPKVKNAW SILPRTKPVR MLELLSAEDS RYVRQQIAEK
2281: LKGTYSESLA KNVFEYFQYG GEVAGHGINN ATTGSVQQPE PAILFEFRSV PSALSDFVPK
2341: TASTVKVDVK ALDHFDSASR KAIITEVNAL VSGSEDFDAW YQEYRASKGQ PPVKNPKSSA
2401: SANHKAEWLM TQHAEQWAKI TAPYTDNHET LTSTKLASND KEELHALGET SNLENNKQQE
2461: NVASIINTML NDMLPFYALR TERNLLVQEG DEGFEVRAWP GTEDKSKTII LEDPEDAAQH
2521: KAIERFILAN FDNFEQMPDE LFLVDNKVIS HHEGRTHVLA QKVDGAWQYN ATVELMSVTE
2581: LLDAANVTGK IRGESYQQVI DALTDYHASI TEHADYEPES VEKLLNLRKK IEGYVLGHPD
2641: SGRVEAMNSL LNQVNTRLDE VSLLSVAEQT IQAQNSFSRL YDQLEAANLK ESKHLYLDQN
2701: GDFVTKGKGN LANIDLLGSR EAVLEKVKLT VSNEYGQTVA DTIFAGLSAK DLAKDGKGVD
2761: IAGLNKVHQA IEQHLSPVSA TLYIWKPSDH SALGHAALQI GQGRTQLEGQ AAADFNQQNY
2821: VSWWPLGSKS SNISNILNVA TKDQPDLKLR WSDFSQPAHQ NDTLEHDVAS EENDGFGLHD
2881: GDIKLKRFIE KLNAAKGIDA SFKEASEGYA SVLLGNPDML ETTSIPAHVF QPFVEQWNDT
2941: SYDMMDVAHR FAQELRLQAQ RSDDPELLEK RIGNVIRQFA ERALEEIETF KASQADQGRV
3001: FRINLEGLDV AAMQAEWHRL SNDPDARYQL LTKNCSSTVA KVLKAGGADK LIGHTWLPKF
3061: GVWTPTELFN FGQALQEAQL EIAAKKQSHQ VTDVLDALSG NEKPKENVAI ENDGTPPRDK
3121: ESLSPLTRFL NNELYGDKEA RRKIGEITQT LLDHAVEKGE SQKITLQGEA GRLTGYYHQG
3181: TAPSEGETSS PSGKVVLFLH GSGSSAEEQA SAIRNHYQKQ GIDMLAVNLR GYGESDGGPS
3241: EKGLYQDART MFNYLVNDKG IDPSNIIIHG YSMGGPIAAD LARYAAQNGQ AVSGLLLDRP
3301: MPSMTKAITA HEVANPAGIV GAIAKAVNGQ FSVEKNLEGL PKETSILLLT DNEGLGNEGE
3361: KLRTKLTASG YNVTGEQTFY GHEASNRLMS QYADQIVSGL SSSASVDEDL DQQGLDTTST
3421: KDQGISNKND HLQVVDSKEA LADGKILHNQ NVNSWGPITV TPTTDGGETR FDGQIIVQME
3481: NDPVVAKAAA NLAGKHAESS VVVQLDSDGN YRVVYGDPSK LDGKLRWQLV GHGRDHSETN
3541: NTRLSGYSAD ELAVKLAKFQ QSFNQAENIN NKPDHISIVG CSLVSDDKQK GFGHQFINAM
3601: DANGLRVDVS VRSSELAVDE AGRKHTKDAN GDWVQKAENN KVSLSWDAQG EVVAKDERIR
3661: NGIAEGDIDL SRIGVNNVDE PARGAIGDNN DVFDAPEKRK PETEVIANSS SSNQFSYSGN
3721: IQVNVGEGEF TAVNWGTSNV GIKVGTGGFK SLAFGDNNVM VHIGDGESKH SVDIGGYQAL
3781: EGAQMFLGNR NVSFNFGHSN DLILMMDKSI PTPPLVNPFD GAARISGVLQ GIATSGEGED
3841: WLAAQEQQWT LSGAKKFVKD MSGLDQSSSV DYTTLVELDS QNERDSRGLK HDAEATLNKQ
3901: YNQWLSGNGN SGTSQLSRAD KLRQANEKLA FNFAVGGQGA DIQVTTGNWN FMFGDNIQSI
3961: LDTNLGSLFG LMTQQFTATG QAKTTFTYTP QDLPRQLKNK LLGQLAGVGA ETTLADIFGV
4021: DYTASGQIVS RNGQAVDGVA ILKEMLEVIG EFSGDQLQAF VDPAKLLDSL KAGIDMGADG
4081: IKSFAETHGL KEKAPEEEKD NSSVSVNGAN VNSAQGATVA DGNTETAETQ DRAFGFNSLN
4141: LPNLFATIFS QDKQKEMKSL VENLKQNLTA DLLNMKEKTF DFLRNSGHLQ GDGDINISLG
4201: NYNFNWGGDG KDLGAYLGDN NNFWGGRGDD VFYATGKSNI FTGGEGNDMG VLMGRENMMF
4261: GGDGNDTAVV AGRINHVFLG AGDDQSFVFG EGGEIDTGSG RDYVVTSGNF NRVDTGDDQD
4321: YSVTIGNNNQ VELGAGNDFA NIFGNYNRIN AGAGNDVVKL MGYHAVLNGG DGDDHLIATA
4381: ISKFSQFNGG EGRDLMVLGG YQNTFKGGTD VDSFVVSGDV IDNLVEDIRS EDNIVFNGID
4441: WQKLWFERSG YDLKLSILRD PSNDSDQSKF EHIGSVTFSD YFNGNRAQVV IGMSEKDLSG
4501: EREYTMLSDS AIDALVQAMS GFEPQAGDNG FIDSLESKSQ AAISMAWSDV VHKKGLMV