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1.C.8.1.2
Tetanus neurotoxin, TetX, of 1315 aas; secreted (Gupta et al. 2023). Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized, and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.

Accession Number:P04958
Protein Name:TETX aka CTP60
Length:1315
Molecular Weight:150682.00
Species:Clostridium tetani [1513]
Location1 / Topology2 / Orientation3: Secreted1 / Multi-pass membrane protein2
Substrate peptide

Cross database links:

RefSeq: NP_783831.1   
Entrez Gene ID: 1061100   
Pfam: PF01742    PF07951    PF07953    PF07952   
KEGG: ctc:pE88_60   

Gene Ontology

GO:0005576 C:extracellular region
GO:0004222 F:metalloendopeptidase activity
GO:0050827 F:toxin receptor binding
GO:0008270 F:zinc ion binding
GO:0051609 P:inhibition of neurotransmitter uptake
GO:0009405 P:pathogenesis
GO:0006508 P:proteolysis

References (9)

[1] “Tetanus toxin: primary structure, expression in E. coli, and homology with botulinum toxins.”  Eisel U.et.al.   3536478
[2] “The complete nucleotide sequence of tetanus toxin.”  Fairweather N.F.et.al.   3774547
[3] “The genome sequence of Clostridium tetani, the causative agent of tetanus disease.”  Brueggemann H.et.al.   12552129
[4] “Cloning, nucleotide sequencing, and expression of tetanus toxin fragment C in Escherichia coli.”  Fairweather N.F.et.al.   3510187
[5] “Arrangement of disulfide bridges and positions of sulfhydryl groups in tetanus toxin.”  Krieglstein K.et.al.   2108021
[6] “Limited proteolysis of tetanus toxin. Relation to activity and identification of cleavage sites.”  Krieglstein K.G.et.al.   1935979
[7] “Tetanus toxin is a zinc protein and its inhibition of neurotransmitter release and protease activity depend on zinc.”  Schiavo G.et.al.   1396558
[8] “Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin.”  Schiavo G.et.al.   1331807
[9] “Structure of the receptor binding fragment HC of tetanus neurotoxin.”  Umland T.C.et.al.   9334741
Structure:
1A8D   1AF9   1D0H   1DFQ   1DIW   1DLL   1FV2   1FV3   1YVG   1YXW   [...more]

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MPITINNFRY SDPVNNDTII MMEPPYCKGL DIYYKAFKIT DRIWIVPERY EFGTKPEDFN 
61:	PPSSLIEGAS EYYDPNYLRT DSDKDRFLQT MVKLFNRIKN NVAGEALLDK IINAIPYLGN 
121:	SYSLLDKFDT NSNSVSFNLL EQDPSGATTK SAMLTNLIIF GPGPVLNKNE VRGIVLRVDN 
181:	KNYFPCRDGF GSIMQMAFCP EYVPTFDNVI ENITSLTIGK SKYFQDPALL LMHELIHVLH 
241:	GLYGMQVSSH EIIPSKQEIY MQHTYPISAE ELFTFGGQDA NLISIDIKND LYEKTLNDYK 
301:	AIANKLSQVT SCNDPNIDID SYKQIYQQKY QFDKDSNGQY IVNEDKFQIL YNSIMYGFTE 
361:	IELGKKFNIK TRLSYFSMNH DPVKIPNLLD DTIYNDTEGF NIESKDLKSE YKGQNMRVNT 
421:	NAFRNVDGSG LVSKLIGLCK KIIPPTNIRE NLYNRTASLT DLGGELCIKI KNEDLTFIAE 
481:	KNSFSEEPFQ DEIVSYNTKN KPLNFNYSLD KIIVDYNLQS KITLPNDRTT PVTKGIPYAP 
541:	EYKSNAASTI EIHNIDDNTI YQYLYAQKSP TTLQRITMTN SVDDALINST KIYSYFPSVI 
601:	SKVNQGAQGI LFLQWVRDII DDFTNESSQK TTIDKISDVS TIVPYIGPAL NIVKQGYEGN 
661:	FIGALETTGV VLLLEYIPEI TLPVIAALSI AESSTQKEKI IKTIDNFLEK RYEKWIEVYK 
721:	LVKAKWLGTV NTQFQKRSYQ MYRSLEYQVD AIKKIIDYEY KIYSGPDKEQ IADEINNLKN 
781:	KLEEKANKAM ININIFMRES SRSFLVNQMI NEAKKQLLEF DTQSKNILMQ YIKANSKFIG 
841:	ITELKKLESK INKVFSTPIP FSYSKNLDCW VDNEEDIDVI LKKSTILNLD INNDIISDIS 
901:	GFNSSVITYP DAQLVPGING KAIHLVNNES SEVIVHKAMD IEYNDMFNNF TVSFWLRVPK 
961:	VSASHLEQYG TNEYSIISSM KKHSLSIGSG WSVSLKGNNL IWTLKDSAGE VRQITFRDLP 
1021:	DKFNAYLANK WVFITITNDR LSSANLYING VLMGSAEITG LGAIREDNNI TLKLDRCNNN 
1081:	NQYVSIDKFR IFCKALNPKE IEKLYTSYLS ITFLRDFWGN PLRYDTEYYL IPVASSSKDV 
1141:	QLKNITDYMY LTNAPSYTNG KLNIYYRRLY NGLKFIIKRY TPNNEIDSFV KSGDFIKLYV 
1201:	SYNNNEHIVG YPKDGNAFNN LDRILRVGYN APGIPLYKKM EAVKLRDLKT YSVQLKLYDD 
1261:	KNASLGLVGT HNGQIGNDPN RDILIASNWY FNHLKDKILG CDWYFVPTDE GWTND