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1.E.20.1.4
Holin of 115 aas and 3 TMSs, pyocin R2 holin.  Functions in DNA release within biofilms (Wang et al. 2016).  A gene cluster encoding a bacteriophage-derived pyocin and its lysis cassette was upregulated in phenozine producing strains. A holin encoded in this gene cluster was found to contribute to the release of eDNA in biofilm matrices, demonstrating that the influence of the phenozine, 2-OH-PCA, on eDNA production is due in part to cell autolysis as a result of pyocin production and release (Wang et al. 2016).

Accession Number:J2YPD8
Protein Name:Pyocin R2, holin
Length:115
Molecular Weight:12263.00
Species:Pseudomonas chlororaphis subsp. aureofaciens 30-84 [1038921]
Number of TMSs:3
Substrate

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FASTA formatted sequence
1:	MTNEQQALLD MPIWLVIVLA LVGGVSGEMW RADKEGARGW LLLRRLALRS GACVICGVST 
61:	IMLLYAAGVS IWTAGAFGCL TAMAGADVAI GLYERWAAKR LGVCEVPPKD PRSDA