TCDB is operated by the Saier Lab Bioinformatics Group

1.E.27 The BhlA Holin (BhlA Holin) Family

A 71 aa BhlA bacteriocin-related holin-like peptide with 1 N-terminal TMS is encoded upstream of a gene encoding an 86 aa holin-like peptide, XhlB in the genome of Bacillus pumilus strain WAPB4 (Aunpad and Panbangred 2012). XhlB is composed of two putative transmembrane domains separated by a β-turn and numerous charged residues in the C-terminus. Dual start motifs were found in both BhlA and XhlB.  To analyze the effect of BhlA on bacteria cells, its ORF was cloned and expressed in Escherichia coli. Expression of BhlA was toxic, and the site of action was in the cell membrane, causing death by membrane disruption as demonstrated by transmission electron microscopy (Aunpad and Panbangred 2012).

References associated with 1.E.27 family:

Anthony, T., G.S. Chellappa, T. Rajesh, and P. Gunasekaran. (2010). Functional analysis of a putative holin-like peptide-coding gene in the genome of Bacillus licheniformis AnBa9. Arch. Microbiol. 192: 51-56. 19967339
Aunpad, R. and W. Panbangred. (2012). Evidence for two putative holin-like peptides encoding genes of Bacillus pumilus strain WAPB4. Curr. Microbiol. 64: 343-348. 22231453
BrĂ¼ser, T. and D. Mehner-Breitfeld. (2022). Occurrence and potential mechanism of holin-mediated non-lytic protein translocation in bacteria. Microb Cell 9: 159-173. 36262927