TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.1.11.1
The oxalate:formate antiporter.  Residues and TMSs involved in the translocation pathway and substrate binding have been identified (Fu and Maloney 1998; Fu et al. 2001; Ye and Maloney 2002; Wang et al. 2006). Beuming and Weinstein 2005 developed a method to predict the structures of membrane proteins consisting of (1) identifying TMSs from sequence; (2) assigning buried and lipid-exposed faces of the TMSs; and (3) assembling the TMSs into a bundle, based on geometric restraints obtained from EM data. The OxlT structure was modeled (Beuming and Weinstein 2005).

Accession Number:Q51330
Protein Name:OxlT
Length:418
Molecular Weight:44517.00
Species:Oxalobacter formigenes [847]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate

Cross database links:

Pfam: PF07690   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015297 F:antiporter activity
GO:0006811 P:ion transport
GO:0055085 P:transmembrane transport

References (6)

[1] “Cloning, sequencing and expression in Escherichia coli of OxlT, the oxalate:formate exchange protein of Oxalobacter formigenes.”  Abe K.et.al.   8636101
[2] “Structure-function relationships in OxlT, the oxalate/formate transporter of Oxalobacter formigenes. Topological features of transmembrane helix 11 as visualized by site-directed fluorescent labeling.”  Fu D.et.al.   9651403
[3] “Structure/function relationships in OxlT, the oxalate/formate antiporter of Oxalobacter formigenes. Assignment of transmembrane helix 2 to the translocation pathway.”  Ye L.et.al.   11919184
[4] “Structure/function relationships in OxlT, the oxalate-formate transporter of Oxalobacter formigenes. Assignment of transmembrane helix 11 to the translocation pathway.”  Fu D.et.al.   11113128
[5] “Topology of OxlT, the oxalate transporter of Oxalobacter formigenes, determined by site-directed fluorescence labeling.”  Ye L.et.al.   11274108
[6] “Projection structure and molecular architecture of OxlT, a bacterial membrane transporter.”  Heymann J.A.W.et.al.   11500368
Structure:
1ZC7     

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence
1:	MNNPQTGQST GLLGNRWFYL VLAVLLMCMI SGVQYSWTLY ANPVKDNLGV SLAAVQTAFT 
61:	LSQVIQAGSQ PGGGYFVDKF GPRIPLMFGG AMVLAGWTFM GMVDSVPALY ALYTLAGAGV 
121:	GIVYGIAMNT ANRWFPDKRG LASGFTAAGY GLGVLPFLPL ISSVLKVEGV GAAFMYTGLI 
181:	MGILIILIAF VIRFPGQQGA KKQIVVTDKD FNSGEMLRTP QFWVLWTAFF SVNFGGLLLV 
241:	ANSVPYGRSL GLAAGVLTIG VSIQNLFNGG CRPFWGFVSD KIGRYKTMSV VFGINAVVLA 
301:	LFPTIAALGD VAFIAMLAIA FFTWGGSYAL FPSTNSDIFG TAYSARNYGF FWAAKATASI 
361:	FGGGLGAAIA TNFGWNTAFL ITAITSFIAF ALATFVIPRM GRPVKKMVKL SPEEKAVH