2.A.1.21.1
The macrolide (erythromycin; oleandomycin; azithromycin; telithromycin) efflux pump, MefA, of 405 aas and 12 TMSs (Cantón et al. 2005; Bley et al. 2011). Iannelli et al. 2018 suggested that MefA can function with an ATPase, MsrD (TC# 3.A.1.121.6), and therefore function as an ABC drug exporter. However, the data presented seem inconsistent with this suggestion. The two genes encoding these two proteins are adjacent to each other, suggesting that they may function together (Iannelli et al. 2018). Predicted transmembrane proteins with homology to MefA do not complement a mefA deletion in the MefA-MsrD macrolide efflux system in Streptococcus pneumoniae (Fox et al. 2021). Note: MsrD is an ATPase of the ABC superfamily, so it can not be certain that MefA and MsrD function together. Coupling of an MFS carrier with an ABC-type energizer is very rare, maybe non-existent.
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| Accession Number: | P95827 |
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| Protein Name: | MefA aka MEF |
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| Length: | 405 |
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| Molecular Weight: | 44279.00 |
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| Species: | Streptococcus pyogenes, and
OS Streptococcus pneumoniae [1314] |
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| Number of TMSs: | 12 |
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| Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Multi-pass membrane protein2 |
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| Substrate |
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| Pfam: |
PF05977
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[1] “Molecular cloning and functional analysis of a novel macrolide-resistance determinant, mefA, from Streptococcus pyogenes.” Clancy J. et.al. 8971709
[2] “The novel conjugative transposon tn1207.3 carries the macrolide efflux gene mef(A) in Streptococcus pyogenes.” Santagati M. et.al. 12959402
[3] “Structure and distribution of an unusual chimeric genetic element encoding macrolide resistance in phylogenetically diverse clones of group A Streptococcus.” Banks D.J. et.al. 14673771
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1: MEKYNNWKLK FYTIWAGQAV SLITSAILQM AIIFYLTEKT GSAMVLSMAS LLGFLPYAVF
61: GPAIGVLVDR HDRKKIMIGA DLIIAAAGSV LTIVAFYMEL PVWMVMIVLF IRSIGTAFHT
121: PALNAVTPLL VPEEQLTKCA GYSQSLQSIS YIVSPAVAAL LYSVWELNAI IAIDVLGAVI
181: ASITVAIVRI PKLGDRVQSL DPNFIREMQE GMAVLRQNKG LFALLLVGTL YMFVYMPINA
241: LFPLISMDYF NGTPVHISIT EISFASGMLI GGLLLGLFGN YQKRILLITA SIFMMGISLT
301: ISGLLPQSGF FIFVVCCAIM GLSVPFYSGV QTALFQEKIK PEYLGRVFSL TGSIMSLAMP
361: IGLILSALFA DRIGVNHWFL LSGTLIICIA IVCPMINEIR KLDLK